Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
권호 표지
DOI QR코드

DOI QR Code

Composition of Phenolic Compounds in Hypochaeris radica L. Extracts and their Allelopathic Effects on Gramineous Forage Crops

서양금혼초(Hypochaeris radicata L.) 추출물의 화본과 사료작물에 대한 타감작용 효과 및 phenole 화합물의 조성

  • Kim, Og-Yim (Department of Science, College of Natural Resources and Life Sciences, Dong-A University) ;
  • Park, Sun-Ill (Department of Science, College of Natural Resources and Life Sciences, Dong-A University) ;
  • Jung, Ill-Min (College of Life and Environment Science, Konkuk University) ;
  • Ha, Sang-Young (Department of Science, College of Natural Resources and Life Sciences, Dong-A University)
  • 김옥임 (동아대학교 생명자원과학대학) ;
  • 박선일 (동아대학교 생명자원과학대학) ;
  • 정일민 (건국대학교 식량자원학과) ;
  • 하상영 (동아대학교 생명자원과학대학)
  • Published : 2005.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to examine the allelopathic effect of the aqueous extract of Hypochaeris radicata L. on growth of three gramineous forage crops, tall fescue, perennial ryegrass and italian ryegrass. The result on relative elongation ration (RER) of gramineous forage crops treated with the aqueous extract of Hypochaeris radicata L. and the change of quantity generally indicated the inhibitory effect. The inhibitory effect was increased as its concentration was increased. As a result, it is ascertained that the aqueous extract of Hypochaeris radicata L. has allelopathy effect. According to the chemical experiment of the allelochemical substances in Hypochaeris radicata L. by HPLC, there are the differences at each part of plants. However, it is ascertained that there are eleven phenolic compounds, ${\rho}-hydroxybenzoic$ acid, chlorogenic acid, catechin, caffeic acid, syringic acid, salicylic acid, ${\rho}-coumaric$ acid, ferulic acid, hesperidin, trans-cinnamic acid and naringenin. Especially three phenolic acids such as caffeic acid, ferulic acid and naringenin were detected from all part of the plant.

본 연구는 귀화식물인 서양 금혼초의 수용성 추출액이 화본과 사료작물인 tall fescue, perennial ryegrass, italian ryegrass 3 종류의 생장과 수량에 미치는 타감작용 효과를 조사하기 위해 실시하였다. 사료작물 생장에 미치는 서양 금혼초 수용성 추출액의 영향을 상대 생장율(relative elongation ratio; RER)로 조사한 결과, italian ryegrass가 다른 두 종류 보다 생장이 가장 크게 억제 되었다. 생장에 미치는 영향을 엽신중, 생체중 및 건물중으로 조사한 결과, 3종류 사료작물 모두 생장량이 감소하였다. 서양 금혼초 수용성 추출액의 처리 농도가 높을수록 3종류사료작물의 생장량이 크게 억제 및 감소하였는데, 이와 같은 결과로 서양 금혼초 수용성 추출액은 타감작용 효과가 있음이 밝혀졌다. 서양 금혼초에 함유되어 있는 allelochemicals 물질을 HPLC에 의해 성분 분석한 결과, 부위별로 함량과 조성의 차이가 있었으나 ${\rho}-hydroxybenzoic$ acid, chlorogenic acid, catechin, caffeic acid, syringic acid, salicylic acid, ${\rho}-coumaric$ acid, ferulic acid, hesperidin, frans-cinnamic acid, naringenin 등 11종류의 phenolic compounds 성분이 동정되었며, caffeic acid, ferulic acid, naringenin 3종류는 서양 금혼초 모든 부위에 공통적으로 함유하고 있는 것으로 확인되었다.

Keywords

References

  1. Aber, J. D. and Melillo, J. M. (1991) Terrestrial ecosystems. Saunder College Pub. pp. 315-316
  2. Inderjit, K. M. and Dakshini, M. (1992) Interference potential of Pluched lanceolata (Asteraceae): Growth and physiological responses of asparagus bean, Vigna unguiculata var. sesquipendalis. Amer. J. Botany. 79(9), 979-981
  3. Newsome, A. E. and I. R. Noble (1986) Ecological and physiological characteristics of invading species, pp. 1-33, In R. H. Groves and J. J. Burdonc (eds.), Ecology of Biological Invasions, Cambridge Univ. Press
  4. Rejmane, K, M. and Richardson, D. M. (1996) What attributes make some plant species more invasive. Ecology. 77, 1655-1661 https://doi.org/10.2307/2265768
  5. Ko, Y. W. and Chae, J. C. (1999) Ecological characteristics of weed species in agricultural lands of Cheju Island. Kor. J. Weed Sci. 19(3), 211-222
  6. Inderjit. (1996) Plant phenolics in allelopathy. Bot. Rev. 62, 182-210
  7. Newman, E. I. (1978) Allelopathy: Adaptation on accident. pp. 327-342. In Barbone, J. B. (ed.), Biochemical Aspects of Plant and Animal Coevalution. Acad. press, New York
  8. Eskelsen, S. R. and Crabtree, G. D. (1995) The role of allelopathy in buckwheat (Fagopyrum esculentum Moench) inhibition of Canada thistle (Cirsium arvense). Weed Sci. 43, 70-74
  9. Kapustka, R. L. and Rice, E. L. (1976) Acetylene reduction (N2-Fixation) in soil and old field succession in central Oklahoma soil. Biochem. 8, 497-553
  10. Lee, I. K. and Monsi, M. (1963) Ecological studies on Pinus densiflora forest. 1. Effect of plant substances on the florestic composition of the undergrowth. Bot. (Tokyo). 76, 400-413 https://doi.org/10.15281/jplantres1887.76.400
  11. Wu, L., X. Guo and Harivondi, M. A. (1998) Allelopathic effects of phenolic acids detected in buffalograss (Buchloe dactylaides) clippings on growth of annual bluegrass (Poa annua) and buffalograss seedlings. Environmental and Experimental Botany 39, 159-167 https://doi.org/10.1016/S0098-8472(97)00040-3
  12. Kim, Y. O. and Lee, H. J. (1996) Identification and effects of phenolic compounds from some plants. Kor. J. Ecole. 19. 329-340
  13. Lee, H. J., Kim, Y. O., and Chang, N. K. (1997) Allelopathic effects on seed germination and fungus growth from the secreting substances of some plants. Kor. J. Ecol. 20(3), 181-189
  14. Rice, E. L. (1984) Allelopathy. 2nd ed. Academic Press, New York and London
  15. Lodhi, M. A. K. (1976) Kolo of allelopathy as expressed by dominating tree in a low land forest in controlling the productivity and pattern of herbaceous growth. Amer. J. Bot. 63, 1-8 https://doi.org/10.2307/2441664
  16. Blum, U. and B. R. Dalton (1985) Effect of ferulic acid, an allelopathic compound, one leaf expansion of cucumber seedling grown in nutrient culture. J. Chem. Ecol. 11, 279-302 https://doi.org/10.1007/BF01411415
  17. Einhellig, F. A. and Rasmussen, J. A. (1973) Allelopathic effects of Rumex crispus on Amaranthus retroflexus grain sorghum and field com. Amer. Mid. Nat. 90, 79-86 https://doi.org/10.2307/2424268
  18. Kil, B. S. (1993) Effects of naturally occurring chemicals from Pinus koraiensis on callus induction and germiculture. Kor. J. Ecol. 16(3), 275-285
  19. Kim, Y. O. (1995) Effect of leaf extract from pinus rigida on morphological changes of root tips. J. Plant. BioI. 38(1), 73-78
  20. Kim, H. Z. and Kim, J. H. (2001a) The allelopathic effects of aqueous chemicals of Ambrosia arternisiifolia on selected plants. Kor. J. Ecol. 24(1), 64-73
  21. Kil, B. S and K. W. Yun. (1992) Allelopathic effects of water extracts of Artemisia primceps var. orientalis on selected plant species. J. Chem. Ecol. 18, 39-51 https://doi.org/10.1007/BF00997163
  22. Yun, K. W., B. Vir, and D. H. Han. (1993) Phytotoxic and antimicrobial activity of volatile constituents of Artemisia priceps var. orientalis. J. Chem. Ecole. 19, 2757-2767 https://doi.org/10.1007/BF00980705
  23. Al-Naib, F. A. and E. L. Rice (1971) Alleopathic effect of Platanus oecidentalis Bull. Torrey Bot. Club 98, 75-82 https://doi.org/10.2307/2483770
  24. Kuo, C. G., M. H. Chou, and H. G. Park (1981) Effect of chinese cabbage residue on mungbean. Plant and Soil. 61, 473-477 https://doi.org/10.1007/BF02182027
  25. Jin, H. Z. and Kim, J. H. (2001) The allelopathic effects of aqueous chemicals of Ambrosia artemisiifolia on selected plants. Kor. J. Ecol. 24(1), 67-73
  26. Lee, J. H. and S. R. Lee (1994) Analysis of phenolic substances content in Korean plant foods. J. Food Sci. Technol. 26, 310-316
  27. Wieslaw, O., C. Y. Lee., Antoni, W., and Price, K.R. (1988) Identification of some phenolic compounds in apples. J. Agrie. Food Chem. 36, 430-432 https://doi.org/10.1021/jf00081a007
  28. Banwart, W. L., Porter, P. M., Granato, T. C. and Hassett, J. J. (1985) HPLC separation and wavelength area ratios of more than 50 phenolic acids and flavonoids. Jourmal of Chemical Ecology. 11(3), 383-395 https://doi.org/10.1007/BF01411424
  29. Kil, B. S. and Yoo, H. G. (1996) Identification and growth inhibition of phytotoxic substances from Artemisia scoparia. Kor. J. Ecol. 19, 295-304
  30. Baskin, J. M and Wilf, F. T. (1967) Psoraen, an inhibition in the seeds of Psoralea subaculis (Leguminosae). Phytochemistry 6, 1209-1213 https://doi.org/10.1016/S0031-9422(00)86083-5
  31. Rice, E. L. and Pancholy, S. K. (1974) Inhibition of nitrification by climaxecosyste-ms. III. Inhibitors other than tannins. Amer. J. Bot. 61, 1095-1103 https://doi.org/10.2307/2441927
  32. Yun, K. W. and Mauri, M. A. (1997) Allelopathic potential of Artemisia campestris ssp. caudata on lake Huron sand dunes. Can. J. Bot. 75, 1903-1912 https://doi.org/10.1139/b97-902
  33. Lee, I. H., Kim, I. T., and Kim, Y. O. (2001) Allelopathic effects of extracts of Trifolium repens on the seed germination and seeding growth of Zoysia japonica. Kor. J. Ecol. 24(3), 125-130