Maxillofacial Plastic and Reconstructive Surgery

  • 제27권4호
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  • Pages.307-314
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  • 2005
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  • 2288-8101(pISSN)
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  • 2288-8586(eISSN)
대한악안면성형재건외과학회 (Korean Association of Maxillofacial Plastic and Reconstructive Surgeons)
권호 표지

타이타늄 임프란트 주위 골유착 관여 유전자의 발현: BMP2, BMP4

EXPRESSION OF OSSEOINTEGRATION-RELATED GENES AROUND TITANIUM IMPLANT: BMP2, BMP4

  • 심정환 (분당서울대병원 구강악안면외과) ;
  • 지유진 (가톨릭대학교 성빈센트병원 치과 구강악안면외과) ;
  • 송현철 (가톨릭대학교 성빈센트병원 치과 구강악안면외과)
  • Shim, Cheong-Hwan (Dept. of Oral & Maxillofacial surgery, Seoul National University Bundang Hospital) ;
  • Jee, Yu-Jin (Div. of Oral and Maxillofacial Surgery, Dept.of Dentistry, St. Vincent's Hospital, The Catholic University of Korea) ;
  • Song, Hyun-Chul (Div. of Oral and Maxillofacial Surgery, Dept.of Dentistry, St. Vincent's Hospital, The Catholic University of Korea)
  • 발행 : 2005.07.30
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초록

After dental implant are planted into their bony site among the various growth factors associated with bone formation. BMP is expressed in the bone surrounding the implant fixture. By taking a close look at BMP2, BMP4 which are growth factors that take put in bone formation, its histologic features and radiographic bone healing patterns we would like to examine the mechanism of osseointegration. We randomly used 8 male and female house rabbit amd used diameter 5 mm height spiral shaped implants(Ostem, Korea) for animal use handled as a resorbable blast machined(RBM) surface and machined surface. 2group were formed and each group had RBM surface and machined surface implant or a simple bone cavity. After 3, 7, 14 and 28 days post surgery 2 objects were sacrificed from each group and histologic specimens were acquired. RT-PCR analysis was conducted and after H&E staining the extent of osseointegration was measured applying a histologic feature and histomorphometric analysis program. Quanitity one -4.41(Bio-Rad, USA) was used after scanning the PCR product image of the growth factors manifested in each group. According to the histomorphometric features the RBM, Machined surface group showed increased contact between bone and implant surface at 3, 7, 14 and 28 days after surgery. The BMP2 level increased in both experiment groups but remained unchanged in the contrast group. BMP4 levels stayed steady after the early post implantation period for RBM but showed decreased in the machined surface group and contrast group. The amount of contact between bone and implant surface increased with the passage of time. BMP2, BMP4 were expressed in both experimental group and contrast group. These growth factors play a role in osseointegration of implant.

키워드

참고문헌

  1. Clokie ML, Warshawasky H : Morphologenic and radioautugraphic studies of bone formation in relation to titanium implants using the rat tibia as a model. Int J Oral Maxillofac Implants 10 : 1, 1995
  2. Ysander M, Branemark R, Olmarker K et al : Intramedullary osseointegeration: Development of a rodent model and study of histology and neuropeptide changes around titanium implants. J Rehab Res Develop 38 : 183, 2001
  3. Fiorellini JP, Buser D, Riley E et al : Effect on bone healing of bone morphogenetic protein placed in combination with endosseous implants: A pilot study in beagle dogs. Int J Periodont Rest Dent 21 : 41, 2001
  4. Won MK, Chang KS, Kim YS et al : An experimental study of newly designed implant with RBM surface in the rabbit tibia: resonance frequency analysis and removal torque study. J Kor Acad Prothodont 4 : 720, 2003
  5. Lian JB, Stein GS : The developmental stages of osteoblast growth and differentiation exhibit selective responses of genes to growth factor(TGF$\beta1$)and hormones( Vitamin D and glucocorticoids). J Oral Impl 19 : 95, 1993
  6. Clokie ML, Bell RC : Recombinant human transforming growth factor $\beta$-1and its effects on osseointegration. J Craniofac Surg 14 : 268, 2003 https://doi.org/10.1097/00001665-200305000-00003
  7. Koka S, Vance JB, Maze GI : Bone growth factors: Potentional for Use as an Osseointegration Enhancement Technique(OET). J West Soc Periodontol Periodontal Abstr 43 : 97, 1995
  8. Buser D, Dahlin C, Schenk RK : Guided bone regeneration in implant dentistry,1996, p67
  9. Sela J, Gross UM, Kohavi D et al : Primary mineralization at the surfaces of implant. Crit Rev Oral Biol Med 11 : 423, 2000 https://doi.org/10.1177/10454411000110040301
  10. Schliephake H : Bone growth factors in maxillofacial skeletal reconstruction. Int J Oral Maxillofac Surg 31 : 469, 2002 https://doi.org/10.1054/ijom.2002.0244
  11. Hunt DR, Jovanovic SA, Wikesjo UME et al : Hayluronan supports recombinant human bone morphogenetic protein- 2 induced bone reconstruction of advanced alveolar ridge defects in dogs. A pilot study. J Periodontol 72 : 651, 2001 https://doi.org/10.1902/jop.2001.72.5.651
  12. Marukawa E, Asahina I, Oda M et al : Functional reconstruction of the non-human private mandible using recombinant human bone morphogenetic protein-2. Int J Oral Maxillofac Surg 31 : 287, 2002 https://doi.org/10.1054/ijom.2001.0207
  13. Geiger M, Li RH, Friess W : Collagen sponges for bone regeneration with rhBMP-2. Adv Drug Deliv Rev 55 : 1613, 2003 https://doi.org/10.1016/j.addr.2003.08.010
  14. Saito E, Saito A, Kawanami M : Favorable healing following space creation in rhBMP-2-induced periodontal regeneration of horiozontal circumferential defects in dogs with experimental periodontitis. J periodontol 74 : 1808, 2003 https://doi.org/10.1902/jop.2003.74.12.1808
  15. Wildemann B, Kandziora F, Krummrey G et al : Local and controlled release of growth factors(combination of IGF-I and TGF-beta I, and BMP-2alone)from a polyactide coating of titanium implants does not lead to ectopic bone formation in sheep muscle. J Control Release 95 : 249, 2004 https://doi.org/10.1016/j.jconrel.2003.11.014
  16. Nakase T, Nomura S, Yoshikawa H et al : Transient and Localized Expression of Bone Morphogenetic Protein 4 Messenger RNA During Fracture Healing. J Bone Miner Res 9 : 651, 1994 https://doi.org/10.1002/jbmr.5650090510
  17. Lugero GG, Caparbo VF, Guzzo ML et al : Histomorphometric evaluation of titanium implants in osteoporotic rabbits. Implant Dent 9 : 303, 2000 https://doi.org/10.1097/00008505-200009040-00005
  18. Matin K, Nakamura H, Iris K et al : Impact of recombinant human bone morphologenetic protein-2 on residual ridge resorption after tooth extraction: An experimental study in the rat. Int J Oral Maxillofac Implants 16 : 400, 2001
  19. Schliephake H : Bone growth factors in maxillofacial skeletal reconstruction. Int J Oral Maxillofac Surg 31 : 469, 2002 https://doi.org/10.1054/ijom.2002.0244
  20. Nakase T, Nomura S, Yoshikawa H et al : Transient and Localized Expression of Bone Morphogenetic Protein 4 Messenger RNA During Fracture Healing. J bone Miner Res 9 : 651, 1994 https://doi.org/10.1002/jbmr.5650090510
  21. Kieswetter K, Schwartz Z, Hummert TW et al : Surfaces roughness modulates the local production of growth factors and cytokines by osteoblast-like MG-63 cells. J Biomed Mater res 32 : 55, 1996 https://doi.org/10.1002/(SICI)1097-4636(199609)32:1<55::AID-JBM7>3.0.CO;2-O
  22. Schwartz Z, Kieswetter K, Dean DD et al : Underlying mechanisms at the bone-surface interface during regeneration. J periodont Res 32 : 166, 1997 https://doi.org/10.1111/j.1600-0765.1997.tb01399.x
  23. Spyrou P, Papaioannou S, Hampson G et al : Cytokine release by osteoblast-like cells cultured on implant discs of varying alloy compositions. Clin Oral Impl Res I : 623, 2002
  24. Ogawa T, Sukotjo C, Nishimura I : Modulated bone matrix-related gene expression is associated with differences in interfacial strength of different implant surface roughness. J prosthodont 11 : 241, 2002 https://doi.org/10.1053/jopr.2002.129772
  25. Schneider GB, Perinpanayagam H, Clegg M et al : Implant surface roughness affects osteoblast gene expression. J Dent Res 82 : 372, 2003 https://doi.org/10.1177/154405910308200509