미생물학회지 (Korean Journal of Microbiology)

  • 제41권3호
  • /
  • Pages.236-238
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  • 2005
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

광조건이 Codyceps militaris의 Cordycepin 생산에 미치는 영향

Effect of Light Conditions on Production of Cordycepin of Cordyceps militaris

  • Jo Sung-Jun (Department of Biology, Graduate School, Graduate School, Dongguk University) ;
  • Lee Tae-Hee (Department of Biology, Graduate School, Graduate School, Dongguk University) ;
  • Chae Dae-Hoon (Dongseo Pharm. Co.,) ;
  • Han Yeong-Hwan (Department of Life Science, College of Natural Science, Dongguk University)
  • 발행 : 2005.09.01
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초록

Cordyceps militaris의 액체배양시 cordycepin(3'-deoxyadenosine) 생산성에 영향을 주는 광조건의 영향을 조사하였다. 사용된 Cordyceps 속균중에서 C. militaris KCTC 6064가 우수한 cordycepin 생산성을 보여주었다. 광조건(1,000 lux)에서 YM배지에 C.militaris KCTC 6064을 120시간 배양한 결과, 51.6 mg/l의 가장 우수한 cordycepin 생산성을 보여주었다. 광조건에서의 cordycepin 생산성은 96시간 배양까지는 증가되었으나, 96시간 이후에는 cordycepin 생산이 감소하였다.

The effect of light on production of cordycepin (3'-deoxyadenosine) was investigated using Cordyceps militaris. Among Cordyceps spp. tested, C. militaris KCTC 6064 was the best for cordycepin production, After 120-hr cultivation of C. militaris KCTC 6064 at $24^{\circ}C$ in YMG broth with light (1,000 lux), 51.6 mg/l of cordycepin was produced. In general, production of cordycepin with light illumination was better than that without light. Cordycepin production under light illumination was enhanced up 96-hr cultivation, whereas after 96-hr, cordycepin production was decreased.

키워드

참고문헌

  1. Ahn, Y.J., S.J. Park, S.G. Lee, S.C. Shin, and D.H. Choi. 2000. Cordycepin: selective growth inhibitor derived from liquid culture of Cordyceps militarlis against Clostridium spp. J. Agric. Food Chem. 48, 2744-2748 https://doi.org/10.1021/jf990862n
  2. Cory, J.G., R.J. Suhadolinik, B. Resnick, and M.A. Rich. 1965. Incorporation of cordycepin into ribonucleic acid of human tumor cells. Biochem. Biophys. Acta. 103, 646-653 https://doi.org/10.1016/0005-2787(65)90085-7
  3. Cunningham, K.G., S.A. Hutchinson, W. Manson, and F.S. Spring. 1951. Cordycepin a metabolic product form cultures of Cordyceps militaris. J. Chem. Soc. 2299-2300 https://doi.org/10.1039/jr9510002299
  4. Frederiksen, S., H. Malling, and H. Klenow. 1965. Isolation of 3'-deoxyadenosine (cordycepin) from the liquid medium of Cordyceps militaris. Biochim. Biophys. Acta. 95, 189-193 https://doi.org/10.1016/0005-2787(65)90483-1
  5. Mueller, W.E.G., B.E. Weiler, R. Charubala, W. Pfleiderer, L. Leserman, R.W. Sobol, R.J. Suhadolnik, and H.C. Schroeder. 1991. Cordycepin analogues of 2'5'-oligoadenylate inhibit human immunodeficiency virus infection via inhibition of reverse transcriptase. Biochem. Wash. 30, 2027-2033 https://doi.org/10.1021/bi00222a004
  6. Penman, S. and M. Rosbach. 1970. Messenger and heterogeneous nuclear RNA in HeLa cells: differential inhibition by cordycepin. Proc. Natl. Acad. Sci. USA. 67, 1878-1885
  7. Stephen, H., C. Don, D.J. James, and A.M. Closkey. 1966. Further evidence on the structure of cordycepin. Biochimi. Biophys. Acta.117, 480-482 https://doi.org/10.1016/0304-4165(66)90101-2
  8. Suger, A.M. and R.P. Mccaffrey. 1998. Antifungal activity of 3'-deoxyadenosine (cordycepin). Antimicrob. Agents Chemother. 42, 1424-1427
  9. Sung, J.M., Y.S. Choi, B. Bhushan, and Y.J. Park. 2002. Investigation on artificial fruiting of Cordyceps militaris. Kor. J. Mycol. 30, 6-10 https://doi.org/10.4489/KJM.2002.30.1.006
  10. Trigg, P., W.E. Gutteridge, and J. Williamson. 1971. The effect of cordycepin on malaria parasites. Trans. R. Soc. Trop. Med. Hyg. 65, 514-520 https://doi.org/10.1016/0035-9203(71)90162-3
  11. Ying, J., X. Mao, Q. Ma, Y. Zong, and H. When. 1987. Icons of medical fungi form China. Science press. Beijing, China. 60-85