Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effect of Diluents for Cold Storage of Olive Flounder (Paralichthys olivaceus) Sperm

희석액벼려 넙치(Paralichthys olivaceus) 정자의 냉장보존 효과

  • LIM Han Kyu (Aquaculture Management Team, National Fisheries Research and Development Institute) ;
  • AN Cheul Min (Aquaculture Management Team, National Fisheries Research and Development Institute) ;
  • SON Maeng Hyun (Aquaculture Management Team, National Fisheries Research and Development Institute) ;
  • PARK Min-Woo (Aquaculture Management Team, National Fisheries Research and Development Institute) ;
  • PARK Yun Jung (Aquaculture Management Team, National Fisheries Research and Development Institute)
  • 임한규 (국립수산과학원 양식관리팀) ;
  • 안철민 (국립수산과학원 양식관리팀) ;
  • 손맹현 (국립수산과학원 양식관리팀) ;
  • 박민우 (국립수산과학원 양식관리팀) ;
  • 박윤정 (국립수산과학원 양식관리팀)
  • Published : 2005.08.01
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Abstract

The effects of diluents composition on cold storage for olive flounder (Paralichthys olivaceus) sperm were examined in terms of the swimming speed of sperm, the percentage of mobile sperm, and fertilization rates. The following results indicated that cold storage methods with fresh conditions could be employed in olive flounder milt preservation. The preserved sperm of olive flounder that was diluted I: 10 with artificial seminal plasma II (ASP II) and Stein's solution (SS) at $0^{\circ}C$ remained motile for 30 days. The most effective condition for cold storage was ASP II and SS at 0 $0^{\circ}C$ for the sperm, although there is no significant difference statistically. No difference in the fertilization rate was found between fresh sperm and the preserved ones with ASP I, II and SS at 10 days post-storage.

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References

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