한국초지조사료학회지 (Journal of The Korean Society of Grassland and Forage Science)

  • 제25권3호
  • /
  • Pages.191-198
  • /
  • 2005
  • /
  • 2287-5824(pISSN)
  • /
  • 2287-5832(eISSN)
한국초지조사료학회 (The Korean Society of Grassland and Forage Science)
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오차드그래스의 종자배양에 있어서 식물생장조절물질과 항산화제가 캘러스유도와 식물체 재분화에 미치는 영향

Effect of Plant Growth Regulators and Antioxidants on Callus Induction and Plant Regeneration from Seed Culture of Orchardgrass

  • 이기원 (경상대학교 응용생명과학부) ;
  • 이상훈 (경상대학교 응용생명과학부) ;
  • 이동기 (경상대학교 응용생명과학부) ;
  • 우현숙 (경상대학교 응용생명과학부) ;
  • 김도현 (경상대학교 응용생명과학부) ;
  • 최명석 (경상대학교 산림자원과학부) ;
  • 김기용 (농촌진흥청 축산연구소) ;
  • 이효신 (국립산림과학원) ;
  • 이병현 (경상대학교 응용생명과학부)
  • Lee Ki-Won (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee Sang-Hoon (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee Dong-Gi (Division of Applied Life Science, Gyeongsang National University) ;
  • Woo Hyun-Sook (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim Do-Hyun (Division of Applied Life Science, Gyeongsang National University) ;
  • Choi Myung Suk (Division of Forest Science, Gyeongsang National University) ;
  • Kim Ki Young (National Livestock Research Institute) ;
  • Lee Hyoshin (Biotechnology Division, Korea Forest Research Institute) ;
  • Lee Byung-Hyun (Division of Applied Life Science, Gyeongsang National University)
  • 발행 : 2005.09.01
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초록

오차드그래스의 최적 조직배양조건을 확립하기 위하여 3가지 품종의 성숙종자로부터 배발생 캘러스 유도 및 식물체 재분화에 미치는 배지첨가물질의 영향을 조사하였다. 성숙종자로 부터 배발생 캘러스 유도시 첨가되는 생장조절 물질로는 3mg/L 2,4-D 또는 dicamba와 0.1mg/L BA를 혼용첨가했을 때가 단용첨가구에 비해 높은 배발생 캘러스 유도효율을 나타내었다. 품종별 식물체 재분화 효율은 'Roughrider'가 가장 높게 나타났으며, 'Frode'가 중간정도, 'Frontier'가 가장 낮은 효율을 나타내었다. 식물체 재분화배지에 항산화물질로서 10mg/L의 $AgNO_3$와 40mg/L의 cysteine을 첨가해준 결과 식물체 재분화율이 증가되었다. 본 연구를 통하여 확립된 오차드그래스의 효율적인 조직배양체계는 유전자 형질전환을 통한 신품종 오차드그래스 개발에 유용하게 응용되어질 수 있을 것이다.

In order to optimize tissue culture conditions for genetic transformation of orchardgrass, the effects of culture medium supplements on tissue culture responses were investigated with mature seeds of 3 cultivars, 'Frode'. 'Roughrider' and 'Frontier' as explant tissues. Callus induction medium containing 3mg/L 2,4-D or 3mg/L dicamba each with 0.1mg/L BA was optimal fer embryogenic callus formation from mature seed and had a strong effect on successive plant regeneration. The regeneration frequency from embryogenic callus among cultivars were descending order of Roughrider > Frode > Frontier. Supplementation of the regeneration media with 10mg/L $AgNO_3$ and 40mg/L cysteine enhanced frequency of plant regeneration. Efficient regeneration system established in this study will be useful fur molecular breeding of orchardgrass through genetic transformation.

키워드

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피인용 문헌

  1. Biological functions of antioxidants in plant transformation vol.44, pp.3, 2008, https://doi.org/10.1007/s11627-008-9110-9