Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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In Vitro Stimulation of Murine Peritoneal Monocytes Induced by Alginates

  • Pasquali Paolo (Dipartimento di Sanita Alimentare ed Animale, Istituto Superiore di Sanita, Istituto Superiore di Sanita) ;
  • Zalcman Amy (Dipartimento di Sanita Alimentare ed Animale, Istituto Superiore di Sanita, Istituto Superiore di Sanita) ;
  • Murtas Susanna (Dipartimento di Sanita Alimentare ed Animale, Istituto Superiore di Sanita, Istituto Superiore di Sanita) ;
  • Adone Rosanna (Dipartimento di Sanita Alimentare ed Animale, Istituto Superiore di Sanita, Istituto Superiore di Sanita) ;
  • Brambilla Gianfranco (Dipartimento di Sanita Alimentare ed Animale, Istituto Superiore di Sanita, Istituto Superiore di Sanita) ;
  • Marianelli Cinzia (Isstituto Zooprofilattico Dell'Umbria e delle Marche) ;
  • Cagiola Monica (Isstituto Zooprofilattico Dell'Umbria e delle Marche) ;
  • Ciuchini Franco (Dipartimento di Sanita Alimentare ed Animale, Istituto Superiore di Sanita, Istituto Superiore di Sanita)
  • Published : 2005.08.01
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Abstract

In this trial we assessed the effect of soluble alginates on murine cells. Mouse peritoneal monocytes were stimulated in vitro with a solution of alginate. The production of $TNF-\alpha$ and nitric oxide (NO), the expression of surface molecules CD80 and CD86, and the ability of monocytes to phagocyte bacteria were assessed, in order to evaluate the effect of alginate on cell functionality. We showed that mouse peritoneal monocytes stimulated with alginate produce NO and $TNF-\alpha$. In addition, alginate is able also to increase their phagocytic activity and to a lesser extent also to increase the expression of CD80. Even with different degrees, it implies that alginates per se act directly on immune response, being able to effectively stimulate proinflammatory activity. These findings corroborate the idea that alginates can represent interesting adjuvants to use to increase the efficacy of antigenic stimulation.

Keywords

References

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