Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Chitosan on the Induction of Renal Dipeptidase (RDPase) from the Proximal Tubules

신장의 근위세뇨관에서 Renal Dipeptidase(RDPase)의 유도에 관한 키토산의 효과

  • Kim, Young-Ho (Dept. of Natural Science, Biology, Chosun University, College of Pharmacy, Research Institute of Drug Development, Chonnam University) ;
  • Yoon, Hyun-Joong (College of Pharmacy, Research Institute of Drug Development, Chonnam University) ;
  • Park, Haeng-Soon (College of Pharmacy, Research Institute of Drug Development, Chonnam University) ;
  • Lee, Myung-Yul (Dept. of Food and Nutrition, Chosun University) ;
  • Kim, Jong-Se (Dept. of Natural Science, Biology, Chosun University)
  • 김영호 (조선대학교 자연과학대학 생물학과, 전남대학교 약학대학 약품개발연구소) ;
  • 윤현중 (전남대학교 약학대학 약품개발연구소) ;
  • 박행순 (전남대학교 약학대학 약품개발연구소) ;
  • 이명렬 (조선대학교 자연과학대학 식품영양학과) ;
  • 김종세 (조선대학교 자연과학대학 생물학과)
  • Published : 2005.08.01
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Abstract

The purpose of this study was to evaluate the effects of chitosan, which is deacetylated derivative of chitin, on the renal function. Renal dipeptidase (RDPase, membrane dipeptidase, dehydropeptidase 1, EC 3.4.13.19) is glycosyl phosphatidyl-inositol (GPI)-anchored ectoenzyme of renal proximal tubular microvilli and was related with renal disease including acute renal failure, pyelitis and nephritis. The released RDPase and Udpase activities were assayed by modified fluorometric method. In vitro experimental groups were consisted of group 1, the concentration ranges of 0, 0.01, 0.05 and $0.1\%$ chitosan only, group 2, the concentration ranges of 1, 2 and 4 mM glycerol only, and group 3, the concentration ranges of 0, 0.01, 0.05 and $0.1\%$ chitosan in the presence of glycerol (4 mM). In vivo experimental groups were consisted of group 1 in which rats were treated with glycerol for the purpose of glycerol-induced renal damage, and group 2 in which rats were treated with chitosan plus glycerol. The RDPase release of 0.01, 0.05, and $0.1\%$ chitosan groups were increased in the concentration dependent manner. The RDPase release of 1, 2, and 4mM glycerol groups were decreased in the concentration dependent manner. Chitosan in the presence of glycerol restored the released RDPase activity in the proximal tubules. In vivo, chitosan inhibited the decrease of RDPase release by glycerol in the kidney and blocked the decrease of Udpase activity by glycerol in urine. These results indicated that chitosan was possible as a functional food to control renal function and its diseases.

기능성 식품소재로서 이미 많이 알려진 키토산이 신장과 관련하여 식품이나 의료용 소재로서 활용이 가능한 지를 알아보기 위하여 신장 기능과 민감하게 관련이 있는 효소인 RDPase, Udpase의 활성을 체내$\cdot$체외 실험을 통하여 관찰하였다. 체외 실험에서 글리세롤에 의해 유도된 RDPase의 유리$\cdot$활성 감소를 다시 회복시키는 것을 관찰하였다. 체내실험에서 글리세롤 투여에 의하여 손상된 신장의 근위세뇨관에서 급격히 증가한 RDPase의 활성을 키토산이 확실하게 감소시키는 것을 관찰할 수 있었다. 키토산을 공급한 쥐의 소변에서의 Udpase의 활성이 증가하는 것을 관찰하였다.

Keywords

References

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