Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Inducible form of Heat Shock Protein 70 (Hsp70) is Expressed in the Rat Cerebellar Synapses in Normal Condition

흰쥐 소뇌 정상 연접에서 열충격단백질70(HSP70)의 표현

  • Cho Sun-Jung (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Jung Jae-Seob (Department of Anatomy, College of Medicine, Dongguk University) ;
  • Jin IngNyol (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Jung Seung Hyun (Department of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Park In Sick (Department of Anatomy, College of Oriental Medicine, Dongguk University) ;
  • Moon Il Soo (Department of Anatomy, College of Medicine, Dongguk University)
  • 조선정 (경북대학교 자연과학대학 미생물학과) ;
  • 정재섭 (동국의대 해부학교실) ;
  • 진익렬 (경북대학교 자연과학대학 미생물학과) ;
  • 정승현 (동국한의대 내과) ;
  • 박인식 (동국한의대 해부학교실) ;
  • 문일수 (경북대학교 자연과학대학 미생물학과)
  • Published : 2005.08.01
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Abstract

Heat shock protein 70 (HSP70) is a multigene family composed of constitutively expressed members(Hsc70) and stress-inducible members (Hsp70). In the mammalian nervous system, a considerable amount of HSPs is also synthesized under normal conditions suggesting that they play an important role in the metabolism of unstressed cells. In this study we examined the expression of Hsp70 in the synapses of rat cerebellar neurons. Immunohistochemistry using specific antibodies revealed that both Hsp70 and Hsc70 are expressed in the cerebellar tissue, with strongest expression in Purkinje cells followed by granule cells. Neurons in deep cerebellar nuclei were also intensely stained by Hsp70 antibody. Immunocytochemical stainings of cultured cerebellar cells showed that Hsp70 is expressed in both Purkinje and granule cells. The expression was punctate in the soma and along dendritic trees, and the punctae were colocalized with those of PSD95, a postsynaptic marker. Immunoblotting also indicates that Hsp70 is associated with the postsynaptic density fraction. Taken together, our results indicate that the Hsp70 is expressed in cerebellar neurons in normal conditions, and that some are localized in the synapses.

열충격단백질 70 (HSP70)은 복수유전자족으로서 통상적으로 표현되는 Hsc70와 스트레스에 의하여 유도되는 Hsp70가 있다. 포유동물의 신경계통에서는 상당한 량의 HSP70가 정상조건에서도 표현되는 것으로 알려져 있다. 본 연구에서는 흰쥐의 소뇌 세포의 연접에서 Hsp70의 표현에 대한 연구를 하였다. 면역조직화학적으로 소뇌절편을 염색하여 관찰한 결과 Hsp70와 Hsc70 모두 표현되었는데, 소뇌 조롱박세포에서 가장 강하게 표현되었으며, 다음으로 소뇌 과립세포에서 강하게 표현되었다. 또한 깊은소뇌핵의 신경세포들도 강하게 염색되었다. 배양한 P1 소뇌신경 세포를 Hsp70 항체로 염색한 결과 Hsp70는 조롱박세포와 과립 세포에서 모두 표현되었으며, 세포체와 가지돌기를 따라 점박이를 형성하였다. 이들 점 박이들은 PSD95 점박이와 같이 위치하였다. 그리고 PSD 분획을 이용한 면역염색에서도 PSD70이 검출되었다. 본 연구결과는 Hsp70이 정상조건에서도 소뇌신경세포의 연접에 존재함을 의미한다.

Keywords

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