DOI QR코드

DOI QR Code

적조생물, Cochlodinium polykrikoides와 Gymnodinium sanguieum의 사멸에 있어 암모니아염의 효과

The Effects of Ammonium Ion and Salts on the Killing of Red Tides Organism; Cochlodinium polykrikoides and Gymnodinium sanguieum

  • 손재학 (신라대학교, 해양바이오전공 Marine-Bio 기능성물질 산업화지원센터)
  • Sohn, Jae-Hak (Major in Marine Biotechnology, Silla University, Marine Biotechnology Center for Bio-Functional Material Industries)
  • 발행 : 2005.08.01

초록

Cell-free culture broth of marine halophilic bacterium, Kordia algicida was shown to possess specific algicidal ability against red tide organism, Cochlodinium polykrikides. Physiochemical characteristics of algicidal material originated in the bacterial culture broth were analyzed that its molecular weight was estimated to a 3,000 dalton and it was stable in heat and pH treatment. The algicidal fraction against C. polykrikoides obtained from gel permeable chromatography contained high concentration of ammonium ion as analyzed by ICP/Mass spectrum. C. polykrikoides by the fraction was quickly lysed within 1 min. It was shown that the effective concentration for algicide against C. polykrikoides was over 1mM of ammonium chloride. On the other hand, other metal ions presented in the algicidal fraction showed no algicidal effect against C. polykrikoides. In additon, ammonium ion exhibited species-specific killing spectrum for two species of red tide organisms, C. polykrikoides and Gymnodinium sanguieum. Therefore, further researches on the killing mechanism against C. polykrikoides exerted by ammonium ion, and subsequent development of replaceable algicidal materials will perform to provide useful tools for the control of red tide.

키워드

참고문헌

  1. 최희구, 김평중, 이원찬, 윤성종, 김학균, 이홍재. 1998. 황토의 유해성 적조생물 Cochlodinium 종의 제거효과. 한국수산과학회지 31, 109-113
  2. APHA. 1992. Standard methods for the examination of water and wastewater, pp. 374-532, 18th eds. APAH, New York
  3. Cole, J. J. 1982. lnteraction between bacteria and algae in aquatic ecosystems. A. Rev. Ecol. Syst. 13, 291-314 https://doi.org/10.1146/annurev.es.13.110182.001451
  4. Berfeld, P. 1955. P 149, In Colowick, S. P. and N. O. Kaplan (eds.), Methods in Enzymology VoL 1, Academic press Inc., New York
  5. Fukami, K, A. Yuzawa, T. Nishijima and Y. Hata. 1992. Isolation of properties of a bacterium inhibiting the growth of Gymnodinium nagasakiense. Nippon Suisan Gakk. 58, 1073-1077 https://doi.org/10.2331/suisan.58.1073
  6. Furuki, M. and M. Kobayashi. 1991. Interaction between Chattonella and bacteria and prevention of this red tide. Marine Pollution Bull. 23, 189-193 https://doi.org/10.1016/0025-326X(91)90673-G
  7. lmai, I., Y. Ishida and Y. Hata. 1993. Killing of marine phytoplankton by a gliding bacterium Cytophaga sp. isolated from the coastal sea of Japan. Mar. Biol. 116, 527-532 https://doi.org/10.1007/BF00355470
  8. Imai, I., Y. Ishida, K. Sakaguchi and Y. Hata. 1995. Algicidal marine bacteria isolated from Northern Hiroshima Bay, Japan Fisheries Scince 61, 628-636
  9. Kato, J., J. Amie, Y. Murata, A. Kuroda, A. Mitsutani and H. Ohtake. 1998. Development of a genetic transformation system for an alga-lysing bacterium. Appl. Environ. Microbiol. 64, 2061-2064
  10. Kawano,Y., Y. Nagawa, H. Nakanishi, H. Nakajima, M. Matsuo and T. Higashihara. 1997. Production of thiotropocin by a marine bacterium, Caulobacter sp. and its antimicroalgal activities. J. Mar. Biotechnol. 5, 225-229
  11. Kim, M-C., I. Yoshinaga, I. Imai, K. Nagasaki, S. Itakura and Y. Ishida. 1998. A close relationship between algicidal bacteria and termination of Heterosigma akshiwo (Raphidophyceae) blooms in Hirosima Bay, Japan. Mar. Ecol. Prog. Ser. 170, 25-32 https://doi.org/10.3354/meps170025
  12. Lovejoy, C., J. P. Bowman and G. M. HaIIegraeff. 1998. Algicidal effect a novel marine Pseudoalteromonas isolate (Class Proteobateria, Gamma subdivision) on harmful algal bloom species of the general Chattonella, Gymnodinium, and Herterosigma. Appl. Environ. Microbiol. 64, 2806-2813
  13. Nagasaki, K., M. Ando, I. Imai, S. Itakura and Y. Ishida. 1994. Virus-like particles in Heterosigma akahiwo (Raphidophyceae): a possible red tide disintegration mechanism. Mar. Biol. 119, 307-312 https://doi.org/10.1007/BF00349570
  14. Nakasaki, K., K. Tarutani and M. Yamaguchi. 1999. Growth characteristics of Heterosigma akashiwo virus and its possible use as a microbiological agent for red tide control. Appl. Environ. Microbiol. 65, 898-902
  15. Sawayama, S., Y. Sako and Y. Ishida. 1993. New inhibitor for mating reaction of Alexandrium catenella produced by marine Alteromonas sp. Nippon Suisan Gakkaishi. 59, 291-294 https://doi.org/10.2331/suisan.59.291
  16. Sohn, J. H., J. H. Lee, H. Yi, J. Chun, K. S. Bae, T-Y. Ahn, and S-J. Kim. 2004. Kordia algicida gen nov., sp. nov., an algicidal bacterium isolated from red tide. Int J. Syst Evol. Bacteriol. 54, 675-680 https://doi.org/10.1099/ijs.0.02689-0
  17. Stein, J. R. 1973. Handbook of phycological methods; culture methods and growth measurements, Cambridge University Press
  18. Suttle, C. A., A. M. Chan and M. T. Cottrel. 1990. Infection of phycoplankton by viruses and reduction of primary productivity. Nature 347, 467-469 https://doi.org/10.1038/347467a0
  19. Zobell, C. E. 1946. Marine microbiology. Chronica Botanica Co., Waltham, Mass