생명과학회지 (Journal of Life Science)

  • 제15권4호
  • /
  • Pages.572-577
  • /
  • 2005
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

음양곽의 항산화작용에 의한 간 보호 효과

The Hepatoprotective Effects of Epimedii Herba through the Antioxidation

  • Ha Bae Jin (Department of Bioscience and Biotechnology, Silla University) ;
  • Kim Hee Jin (Department of Bioscience and Biotechnology, Silla University) ;
  • Lee Sang Hun (Department of Bioscience and Biotechnology, Silla University) ;
  • Ha Jong-Myung (Department of Bioscience and Biotechnology, Silla University) ;
  • Lee Sang-Hyeon (Department of Bioscience and Biotechnology, Silla University) ;
  • Lee Jae-Hwa (Department of Bioscience and Biotechnology, Silla University) ;
  • Lee Dong-Geun (Department of Bioscience and Biotechnology, Silla University) ;
  • Park Eun Kyung (Department of Bioscience and Biotechnology, Silla University) ;
  • Nam Chun Suk (Department of Bioscience and Biotechnology, Silla University)
  • 발행 : 2005.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

음양곽의 항산화 효과를 규명하기 위하여 흰쥐(Sprague-Dawley계)를 사용하여 음양곽 추출물을 2주간 복강내로 투여하고 $CCl_4$에 의한 간독성 경감기전을 혈액 및 간 조직에서의 효소 변동과 간 조직을 병리학적으로 관찰하였다. $CCl_4$의 투여로 AST, ALT, MDA가 증가하였으나 음양곽을 전처리하고 $CCl_4$를 후 투여한 경우에는 AST, ALT, MDA가 감소하였다. $CCl_4$의 투여로 SOD, catalase, GPx가 감소하였으나 음양 곽 전처리 한 후 $CCl_4$를 투여 한 경우에는 SOD, catalase, GPx가 증가하였다. 음양곽은 $CCl_4$로 유도된 free radicals를 scavenging함으로써 간독성을 경감시키는 것으로 사료된다. 위의 생리활성과 항산화계 변화 지표에서 나타난 음양곽의 간기능 보호효과를 형태학적으로 확인하기 위한 조직병리학적 검사결과 음양곽이 세포막의 소기관들을 과산화로부터 보호함으로써 효소활성의 최적구조를 유지시켜 간 손상을 완화시키는 것으로 사료된다.

In this study, the effect of Epimedii Herba (EH) on the antioxidative enzymatic activity was investigated. EH (100mg/kg) was intraperitoneally administered into rats for 2 weeks. On the last day, carbon tetrachloride $(50\%\;CCl_4,\;3.3ml/kg,\;i.p.)$ dissolved in olive oil was injected before 12 hours. EH-pread-ministered and $CCl_4-treated$ (EC) group showed higher inhibitory effect in aminotransferase (AST, ALT) activity compared to $CCl_4-treated (CT)$ group. Superoxide dismutase (SOD) and Catalase in EC group were increased compared to those of CT group. The activity of glutathione peroxidase (GPx) was significantly higher than those of CT group compared to EC group. These results suggest that EH has a hepatoprotective effect through scavenging the free radicals induced by $CCl_4$.

키워드

참고문헌

  1. Aebi, H. 1984. Catalase in vitro, Methods. Enzymology 105, 121-126 https://doi.org/10.1016/S0076-6879(84)05016-3
  2. Beauchamp, C. and Fridovich, I. 1971. Superoxide dismutase : improved assays and an assay applicable to acrylamide gel. Anal Biochem. 44, 276-287 https://doi.org/10.1016/0003-2697(71)90370-8
  3. Butler, T. C. 1990. Reduction of carbon tetrachloride in vivo and reduction of carbon tetrachloride and chloroform in vitro by tissues and tissues constituents. J. Pharmacol. Exp. Ther. 134, 311-319
  4. C. A. Rice-Evans and N. J. Miller, in : C. A. Rice-Evans, L. Packer (eds.). 1998. Flavonoids in Health and Disease. Marcel Dekker, Inc., New York, pp. 199-218
  5. C. S. Jeong, K. W. Jung and J. S. Jeong. 1999. Hepatoprotective Effect of Subfractions of Carthamus tinctorius L. Semen on the Reversal of Biotransformation Enzyme Activities in $CCl_{4}$-induced Hepatotoxic Rats. J. Fd Hyg. Safety. 14(2), 172-178
  6. Chance, B., Sies, H. and Boveris, A. 1979. Hydroperoxide metabolism in mammalian organs. Physiol. Rev. 59, 527-605
  7. Fred, J. Yost, J. and Fridovich, I. 1976. Superoxide and hydrogen peroxide in oxygen damage. Arch. Biochem. Biophys. 175, 514-518 https://doi.org/10.1016/0003-9861(76)90539-7
  8. Fridovich, I. 1986. Biologic effects of the superoxide radicals. Arch. Biochem. Biophysm 247, 1-15 https://doi.org/10.1016/0003-9861(86)90526-6
  9. Gabriel L. Plaa and William R. Hewitt. 1982. Hayes: Principles and Methods of Toxicology. Raben Press, pp. 407-445
  10. Gutteridge, J. M. C., Beard, A. P. C and Quinlan, G. J. 1983. Superoxide-dependent lipid peroxidation. Problem with the use of catalase as a specific probe for Fenton-derived hydroxyl radicals. Biochem. Biophys. Res. Commun. 117, 901-907 https://doi.org/10.1016/0006-291X(83)91681-9
  11. H. Mao, L. Zhang, Y. Wang and X. Li. 2000. Experimental studies of icariin on anticancer mechanism. Zhong Yao Cai. 23(9), 554-556
  12. H. R. Ling, H. Siren, M. L. Riekkola, P. Vuorela, H. Vuorela and R. Hiltunen. 1996. Optimized separation of pharmacologically active flavonoids from Epimedium species by capillary electrophoresis. Journal of Chromatography A 746, 123-129 https://doi.org/10.1016/0021-9673(96)00287-7
  13. H. I. Choi, S. I. Lee, D. K. Ahn and H. C. Kim. 1997. A Study on the Antihypertensive Effect of Epimedii Herba. J. of Herbology 12(1), 35-44
  14. L. Tian, Z. C. Xin, Y. M. Yuan, J. Fu, W. J. Liu and L. L. Wang. 2004. Effects of icariin on intracavernosal pressure and systematic arterial blood pressure of rat. Zhonghua Yi Xue Za Zhi. 84(2), 142-145
  15. Lawrence, R. A. and Burk, R. F. 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem. Biophys. Res. Commun. 71, 952-958 https://doi.org/10.1016/0006-291X(76)90747-6
  16. M. K. Lee, Y. J. Choi, S. H. Sung, D. I. Shin, J. W. Kim and Y. C. Kim 1995. Antihepatotoxic activity of icariin, a major constituent of Epimedium koreanum. Pinata Med. 61(6), 523-526
  17. Lowry, O. H, Rosenbrough. N. J., Farr, A. S. and Randall, R. J. 1951. Protein Measurenment with Folin phenol reagent. J. Biol. Chem. 193, 256-261
  18. McCay, P. B., Lai, E. K., Poyer, J. L., Dubose, C. M. and Janzen, E. G. 1984. Oxygen and carbon-centered free radical formation during carbon tetrachloride metabolism. J. Biol, Chem., 259, 2135-2143
  19. McPhalen, C. A., Vincent, M. G. and Jansonius, J. N. 1992. X-ray structure refinement and comparison of three forms of mitochondrial aspartate aminotransferase. J. Mol. BioI. 225, 495-517 https://doi.org/10.1016/0022-2836(92)90935-D
  20. Ohkawa, A. and Ohishi, N. and Yagi, K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 95, 351-358 https://doi.org/10.1016/0003-2697(79)90738-3
  21. Rosen, D. R., etal. 1993. Mutations with familial amyotrophic lateral sclerosis. Nature 362, 59-62 https://doi.org/10.1038/362059a0
  22. S. S. Kang, J. S. Kim, Y. J. Kang and H. K. Han. 1990. Phytochemical studies on Epimedii Herba (II). Kor. J. Phamracogn. 21(1), 56-59
  23. Tainer, J. A., Getzoff, E. D., Richardson, J. S., and Richardson, D. C. 1983. Structure and mechanism of Cu, Zn, superoxide dismutase. Nature 306, 274-287 https://doi.org/10.1038/306274a0
  24. Takaharu Nomura and Kiyonori Yamaoka. 1999. Low-dose $\gamma$-ray irradiation reduces oxidative damage induced by $CCl_{4}$ in mouse liver. Free Radical Biology & Medicine. 27(11/12), 1324-1333 https://doi.org/10.1016/S0891-5849(99)00180-X
  25. Von, Sonntag. 1987. In 'The Chemical Basis of Radiation of biology' Tylor and Francis.(ed.). London. pp. 31
  26. W. K. Li, P. G. Xiao, G. Z. Tu, L. B. Ma and R. Y. Zhang 1995. Flavonol Glycosides from Epimedium koreanum. Phytochemistry 38(1), 263-265 https://doi.org/10.1016/0031-9422(94)00522-U
  27. Yosjikawa, T., Murakami, M., Yoshida, N., Seto, O. and Kondo, M. 1983. Effects of superoxide dimutase and catalase on disseminated intravascular coagulation in rats. Thromb. Haemostas 50, 869-872

피인용 문헌

  1. Antioxidative Effect of Fermented Rhynchosia nulubilis in Obese Rats vol.30, pp.4, 2015, https://doi.org/10.13103/JFHS.2015.30.4.383