Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effects of EGF, $\beta-ME$, Glucose on the In Vitro Maturation and Development of Porcine NT Embryos

EGF, $\beta-ME$와 Glucose가 돼지 난자의 체외성숙에 미치는 영향과 핵 이식 배의 발생에 대한 영향에 관한 연구

  • Quan J. H. (College of Veterinary Medicine, Chungnam National University) ;
  • Kim S. K. (College of Veterinary Medicine, Chungnam National University)
  • 전연화 (충남대학교 수의과대학) ;
  • 김상근 (충남대학교 수의과대학)
  • Published : 2005.08.01
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Abstract

본 연구는 NCSU-23과 PZM-3 배양액에 EGF, $\beta-ME$와 glucose의 첨가가 돼지 난자의 체외성숙에 미치는 영향과, 배양조건을 다르게 하여 계대배양한 섬유아세포를 이용한 핵이식 배를 다른 배양액과 산소조건에서 배양하였을 때 체외발생율에 미치는 영향을 조사하였다. 난자를 20ng/ml EGF를 첨가 또는 첨가하지 않은 NCSU-23 및 PZM-3 배양액에서 44시간 배양하였을 때 난자의 체외성숙율은 각각 $85.7\pm3.1\%,\;75.2\pm2.8\%,\;87.1\pm2.4\%$와 $78.2\pm2.6\%$였으며 EGF를 첨가한 배양액에서 배양한 난자의 체외성숙율은 EGF를 첨가하지 않은 배양액에서 배양했을 때의 난자보다 높은 체외성숙율을 나타냈다(p<0.05). 난자를 $25{\mu}M\;\beta-ME$를 첨가 또는 첨가하지 않은 NCSU-23 및 PZM-3 배양액에서 44시간 배양하였을 때 난자의 체외성숙율은 각각 $79.5\pm2.6\%,\;74.7\pm2.5\%,\;80.2\pm2.3\%,\;78.6\pm2.7\%$였고 $\beta-ME$를 첨 가한 PZM-3 배양액에서 배양한 난자의 체외성숙율이 가장 높게 나타났다. 난자를 1.5mM glucose를 첨가 또는 첨가하지 않은 NCSU-23 및 PZM-3 배양액에서 44시간 배양하였을 때 난자의 체외성숙율은 각각 $79.2\pm2.3\%,\;75.0\pm2.6\%,\;85.5\pm2.5\%$와 $78.9\pm2.7\%$였고, glucose를 첨가한 PZM-3 배양액에서 배양한 난자의 체외성숙율은 glucose를 첨가하지 않은 PZM-3 배양액에서 배양한 난자보다 높은 체외성숙율을 나타냈다(p<0.05). 핵이식 배를 20ng/m1 EGF, $25{\mu}M\;\beta-ME$ 및 1.5mM glucose를 첨가한 NCSU-23 및 PZM-3 배양액에서 48시간, 144시간 배양하였을 때 2세포기 및 배반포로의 체외발생율은 각각 $56.4\pm2.7\%,\;54.3\pm2.9\%,\;70.5\pm2.1\%,\; 69.6\pm1.5\%$ 및 $12.0\pm1.3\%,\;9.6\pml.7\%,\;10.9\pm2.1\%,\;11.9\pm1.8\%$였다.

Keywords

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