Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Biohydrogen Production from Carbon Monoxide and Water by Rhodopseudomonas palustris P4

  • Oh You-Kwan (Department of Chemical Engineering, Pusan National University, Institute for Environmental Technology and Industry, Pusan National University) ;
  • Kim Yu-Jin (Department of Chemical Engineering, Pusan National University, Institute for Environmental Technology and Industry, Pusan National University) ;
  • Park Ji-Young (Institute for Environmental Technology and Industry, Pusan National University) ;
  • Lee Tae Ho (Institute for Environmental Technology and Industry, Pusan National University) ;
  • Kim Mi-Sun (Biomass Research Team, Korea Institute of Energy Research) ;
  • Park Sunghoon (Department of Chemical Engineering, Pusan National University, Institute for Environmental Technology and Industry, Pusan National University)
  • Published : 2005.06.01
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Abstract

A reactor-scale hydrogen (H2) production via the water-gas shift reaction of carbon monoxide (CO) and water was studied using the purple nonsulfur bacterium, Rhodopseudomonas palustris P4. The experiment was conducted in a two-step process: an aerobic/chemoheterotrophic cell growth step and a subsequent anaerobic $H_2$ production step. Important parameters investigated included the agitation speed. inlet CO concentration and gas retention time. P4 showed a stable $H_2$ production capability with a maximum activity of 41 mmol $H_2$ g $cell^{-1}h^{-1}$ during the continuous reactor operation of 400 h. The maximal volumetric H2 production rate was estimated to be 41 mmol $H_2 L^{-1}h^{-1}$, which was about nine-fold and fifteen-fold higher than the rates reported for the photosynthetic bacteria Rhodospirillum rubrum and Rubrivivax gelatinosus, respectively. This is mainly attributed to the ability of P4 to grow to a high cell density with a high specific $H_2$ production activity. This study indicates that P4 has an outstanding potential for a continuous H2 production via the water-gas shift reaction once a proper bioreactor system that provides a high rate of gas-liquid mass transfer is developed.

Keywords

References

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