Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Studies on Decolorization Process for rhEGF as Cosmetic Ingredient

  • Zhao Xiao-wei (School of Applied Technologies, Wenzhou University) ;
  • Xu Zhi-nan (Institute of Bioengineering, Department of Chemical Engineering and Bioengineering, Zhejiang University) ;
  • Zhou Mao-hong (School of Applied Technologies, Wenzhou University) ;
  • Cen Pei-lin (Institute of Bioengineering, Department of Chemical Engineering and Bioengineering, Zhejiang University)
  • Published : 2005.06.01
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Abstract

A decolorization process using ion exchange chromatography was developed to refine rhEGF as a cosmetic ingredient. A macroreticular resin (D314) was selected, with respect to its high decolorization rate and recovery yield of rhEGF, and the operational conditions of the decolorization process optimized. The optimum conditions were as follows: the rhEGF effluent was ion exchanged at a flow rate of 60.0mL/h, with an effluent pH 5.0, using a chromatographic column (i.d. 16mm) packed with D314, with a 7.5cm in bed height. The decolorization process was carried out under the optimum conditions, and halted when the effluent volume reached 350mL, giving a decolorization rate and recovery yield of rhEGF higher than 67 and $80\%$, respectively. When the decolorization rate exceeded $67\%$, the final product turned out to be white or light yellowish, which was to the satisfaction of the cosmetic standard.

Keywords

References

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