Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of an Improved Recombinant Baculovirus Producing Polyhedra that Contain Bacillus thuringiensis Cry1Ac Crystal Protein

  • Kim Jae-Su (School of Agricultural Biotechnology, College of Agriculture & Life Science, Seoul National University, Dongbu Hannong Chemical Co., Ltd., Agricultural Technology Research Institute (ATRI)) ;
  • Cho Jae-Young (School of Agricultural Biotechnology, College of Agriculture & Life Science, Seoul National University) ;
  • Chang Jin-Hee (School of Agricultural Biotechnology, College of Agriculture & Life Science, Seoul National University) ;
  • Shim Hee-Jin (School of Agricultural Biotechnology, College of Agriculture & Life Science, Seoul National University) ;
  • Roh Jong-Yul (School of Agricultural Biotechnology, College of Agriculture & Life Science, Seoul National University) ;
  • Jin Byung-Ae (College of Natural Resources and Life Sciences, Dong-A University) ;
  • Je Yeon-Ho (School of Agricultural Biotechnology, College of Agriculture & Life Science, Seoul National University)
  • Published : 2005.08.01
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Abstract

A novel recombinant baculovirus, Bactrus, was constructed by the insertion of the Bacillus thuringiensis cry1Ac gene between two polyhedrin genes of Autographa californica nucleopolyhedrovirus (AcNPV) under the control of the polyhedrin gene promoter. Polyhedra produced by Bactrus in insect cells were incorporated with 130 kDa of polyhedrin-Cry1Ac-polyhedrin fusion protein, and 30 kDa of intact polyhedrin, resulting from a homologous recombination between two polyhedrin genes, was also expressed. The insecticidal activity of Bactrus against Spodoptera exigua larvae was similar to that of AcNPV, but it showed significantly higher toxicity towards Plutella xylostella larvae in comparison with that of AcNPV. The expression level of fusion protein and the insecticidal activity of recombinant polyhedra produced by the Bactrus against P. xylostella larvae were decreased after serial passages. In conclusion, the Bactrus had improved insecticidal activity and returned to wild-type AcNPV after several passages.

Keywords

References

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