Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Preparation of Feather Digests as Fertilizer with Bacillus pumilis KHS-1

  • Kim, Jin-Man (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Choi, Yang-Mun (Department of Food Service and Industry, Shinsung College) ;
  • Suh, Hyung-Joo (Department of Food and Nutrition, College of Health Sciences, Korea University)
  • Published : 2005.06.01
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Abstract

The present study was untaken to assess the capacity of Bacillus pumilis KHS-1 to grow on chicken flour and to prepare feather digest as fertilizer. To increase keratinolytic activity, the addition of cysteine (5.0 mM) showed the highest keratinolytic activity (245 unit) among the reducing agents tested. The production of soluble protein (feather digests) paralleled the tendency to the production of keratinolytic protease. In the growth curve of B. pumilis KHS-1 at $30^{\circ}C$ in the feather medium with 5 mM cysteine, the maximum keratinolytic activity of B. pumilis was about 161 units/ml after 84 h of incubation. The maximum enzyme activities were observed at the late logarithmic growth phase, and remained thereafter with little changes. Using 27-day plant growth assays on carrot and Chinese cabbage, feather digests and reference fertilizer were compared. In terms of the length and the weight of the above-ground vegetations, feather digests showed the same effect as that of the fertilizer. Therefore, our investigation shows that the feather digests can be used in agriculture.

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References

  1. American Society for Testing and Materials. 1994. Designation: E1598-94. Standard Practice for Conducting Early Seeding Growth Test. pp. 1061 - 1067. American Society for Testing and Materials
  2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye biding. Anal. Biochem. 72: 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  3. Cheng, S. W, H. M. Hu, S. W Shen, H. Takagi, M. Asano, and Y. C. Tsai. 1995. Production and characterization of keratinase of a feather-degrading Bacillus licheniformis PWD-1. Biosci. Biotechnol. Biochem. 59: 2239-2243 https://doi.org/10.1271/bbb.59.2239
  4. Dalev, P. G. 1994. Utilization of waste feather from poultry slaughter for production of a protein concentrate. Bioresource Technol. 48: 265-267 https://doi.org/10.1016/0960-8524(94)90156-2
  5. Elmayergi, H. H. and R. E. Smith. 1971. Influence of growth of Streptomyces fradiae on pepsin-HCI digestibility and methionine content of feather meal. Can. J. Microbiol. 17: 1067-1072 https://doi.org/10.1139/m71-169
  6. Kim, J. M., W J. Lim, and H. J. Suh. 2001. Featherdegrading Bacillus species from poultry waste. Process Biochem. 37: 287-291 https://doi.org/10.1016/S0032-9592(01)00206-0
  7. Kunert, J. 1992. Effects of reducing agents on proteolytic and keratinolytic activity of enzymes of Microsporium gypseum. Mycoses 35: 343-348 https://doi.org/10.1111/j.1439-0507.1992.tb00892.x
  8. Lin, X., C. Lee, E. S. Casale, and J. C. H. Shih. 1992. Purification and characterization of a keratinase from a feather degrading Bacillus licheniformis strain. Appl. Environ. Microb. 58: 3271-3275
  9. Malviya, H. K., S. Tiwari, R. C. Rajak, and S. K. Hasija. 1993. Keratinolysis by four fungi isolated from the soil and effluent of a gelatin factory at Jabalpur (M.P.). Cryptogamic Bot. 3: 108-116
  10. Marable, B., T. Nagaoka, and T. Ando. 1993. Identification and biological activity of germination-inhibiting long-chain fatty acids in animal-waste composts. Plant Cell Physiol. 34: 605-612
  11. Onifade, A. A., N. A. AI-Sane, A. A. AI-Musallam, and S. AI-Zarban. 1998. A review: Pqtentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources. Bioresource Technol. 66: 1-11 https://doi.org/10.1016/S0960-8524(98)00033-9
  12. Papadopoulos, M. C., A. R. EI Boushy, A. E. Roodbeen, and E. H. Ketelaars. 1986. Effects of processing time and moisture content on amino acid composition and nitrogen characteristics offeather meal. Animal Feed Sci. Technol. 14: 279-290 https://doi.org/10.1016/0377-8401(86)90100-8
  13. Rubaek, G. H., K. Henriksen, J. Petersen, B. Rasmussen, and S. G. Sommer. 1996. Effects of application technique and anaerobic digestion on gaseous nitrogen loss from animal slurry applied to ryegrass (Lolium perenne). J. Agric. Sci. 126: 481-492 https://doi.org/10.1017/S0021859600075572
  14. Salminen, E., J. Rintala, J. Harkonen, M. Kuitunen, H. Hogmander, and A. Oikari. 2001. Anaerobically digested poultry slaughterhouse wastes as fertiliser in agriculture. Bioresource Technol. 78: 81-88 https://doi.org/10.1016/S0960-8524(00)00160-7
  15. Sangali, S. and A. Brandelli. 2000. Isolation and characterization of a novel feather-degrading bacterial strain. Appl. Biochem. Biotechnol. 87: 17-24 https://doi.org/10.1385/ABAB:87:1:17
  16. Shama, G. and P. G. Berwick. 1991. Production of keratinolytic enzymes in a rotating frame bioreactor. Biotechnol. Tech. 5: 359-362
  17. Shih, J. C. H. 1993. Recent development in poultry waste digestion and feather utilization - a review. Poult. Sci. 72: 1617-1620 https://doi.org/10.3382/ps.0721617
  18. Sneath, P. H. A., N. S. Mair, M. E. Sharpe, and J. G. Holt. 1986. Bergey's Manual of Systematic Bacteriology, vol. 2. Williams and Wilkins, Baltimore, U.S.A
  19. Suh, H. J. and H. K. Lee. 2001. Characterization of a keratinolytic serine protease from Bacillus subtilis KS-1. J. Protein Chem. 20: 165-169 https://doi.org/10.1023/A:1011075707553
  20. Suntornsuk, W. and L. Suntornsuk. 2003. Feather degradation by Bacillus sp. FK 46 in submerged cultivation. Bioresource Technol. 86: 239-243 https://doi.org/10.1016/S0960-8524(02)00177-3
  21. Tomarelli, R. M., J. Charney, and M. L. Harding. 1949. The use of azoalbumin as a substrate in the colorimetric determination of peptic and tryptic activity. J. Lab. Clinical Med. 34: 428-433
  22. Vermeulen, J., A. Huysmans, M. Crespo, A. van Lierde, A. de Rycke, and W. Verstraete. 1992. Processing of biowaste by anaerobic composting to plant growth substrate, pp. 147-157. In: Proceedings of the International Symposium on Anareobic Digestion of Solid Waste, Venice, Italy
  23. Williams, C. M., C. S. Richter, J. M. MacKenzie, and J. C. H. Shih. 1990. Isolation, identification and characterization of a feather-degrading bacterium. Appl. Environ. Microbiol. 56: 1509-1515