Plant Resources
- 제8권1호
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- Pages.60-66
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- 2005
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- 1226-8771(pISSN)
Generation and DNA Characterization of High-lysine Mutants by Biochemical Selection from Callus Culture of 'Hwayeongbyeo'
- Yi Gi-Hwan (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
- Choi Jun-Ho (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
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Kim Kyung-Min
(Institution of genetic engineering, Kyungpook National University) ;
- Jeong Eung-Gi (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
- Park Hyang-Mi (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
- Kim Doh-Hoon (Plant Genetics Engineering Division, Donga University) ;
- Ku Yeon Chung (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
- Eun Moo-Young (Rice Functional Genomics Team, NICS, RDA) ;
- Kim Ho-Yeong (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
- Nam Min-Hee (Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA)
- 발행 : 2005.04.01
초록
Lysine is the first essential amino acid for optimal nutrient quality in rice grain. For the narrow genetic diversities of lysine contents in rice, somaclonal variation was the source of mutation in our breeding program. Biochemical selection was conducted using 1 mM S-(2-aminoethyl) cysteine followed by two passages of 5 mM lysine plus threonine in the callus subculture medium. The lysine contents in endosperm of all progenies recovered from the biochemical selection were higher than those of their donor cultivar 'Hwayeongbyeo'. These elevated lysine levels of mutants were successfully transmitted to