대한한의학회지 (The Journal of Korean Medicine)

  • 제26권2호
  • /
  • Pages.241-251
  • /
  • 2005
  • /
  • 1010-0695(pISSN)
  • /
  • 2288-3339(eISSN)
대한한의학회 (The Society of Korean Medicine)
권호 표지

오차산감방이 3T3-L1 adipocyte의 leptin 및 leptin receptor 함량과 differentiation에 미치는 영향

The Effects of Oryungsan-gagampang on Leptin Levels, Leptin Receptor Levels and Differentiation of 3T3-L1 Adipocyte

  • 강중원 (경희대학교 한의과대학 침구학교실) ;
  • 최도영 (경희대학교 한의과대학 침구학교실) ;
  • 박동석 (경희대학교 한의과대학 침구학교실) ;
  • 이재동 (경희대학교 한의과대학 침구학교실)
  • Kang Jung-Won (Department of Acupuncture & Moxibustion, College of Oriental Medicine, KyungHee University) ;
  • Choi Do-Young (Department of Acupuncture & Moxibustion, College of Oriental Medicine, KyungHee University) ;
  • Park Dong-Suk (Department of Acupuncture & Moxibustion, College of Oriental Medicine, KyungHee University) ;
  • Lee Jae-Dong (Department of Acupuncture & Moxibustion, College of Oriental Medicine, KyungHee University)
  • 발행 : 2005.06.01
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초록

Objectives: This experimental study was designed to investig:ue the effects of Oryungsan-gagampang on leptin and leptin receptor levels and differentiation of 3T3-L1 adipocyte. Methods: After 3T3-L1 adipocytes were incubated with various concentrations of Oryungsan-gagampang and Reductil(r) for 7 days, leptin and leptin receptor levels in 3T3-Ll adipocytes were measured by ELISA. To elucidate the mechanism of inhibitory effects of Oryungsan-gagampang on obesity, the 3T3-L1 adipocytes after oil red 0 staining were taken by digital photo system. Results: 1. Oryungsan-gagampang $1,000{\mu}g/ml$ significantly increased leptin levels in 3T3-L1 adipocytes in comparison with the control group (p<0.05), and Oryungsan-gagampang 0.1 10, $1,000{\mu}g/ml$ significantly increased leptin receptor levels in 3T3-L1 adipocytes in comparison with the control group (p<0.05). 2. Oryungsan-gagampang inhibited of differentiation of 3T3-L1 adipocytes. Conclusions: Oryungsan-gagampang showed significant effects on inhibiting differentiation of 3T3-Ll adipocytes, and increasing leptin levels and leptin receptor levels in 3T3-L1 adipocytes. Therefore, Oryungsan-gagampang could be used to treat obesity, but further studies are required.

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