Effect of Chungpaesagan-tang on Ischemic Damage Induced by Middle Cerebral Artery Occlusion in Diabetic Rats

청폐사간탕이 탕요유발 흰주의 뇌허혈손상에 미치는 영향

  • Jeong Chun-geun (Department of Anatomy, College of Oriental Medicine, Kyung Hee University) ;
  • Kim Eun-Young (Department of Anatomy, College of Oriental Medicine, Kyung Hee University) ;
  • Shin Jung-Won (Department of Neuroscience, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Sohn Youngjoo (Department of Gynecology, College of Oriental Medicine, Sangji University) ;
  • Lee Hyun-Sam (Department of Anatomy, College of Oriental Medicine, Kyung Hee University) ;
  • Jung Hyuk-Sang (Department of Anatomy, College of Oriental Medicine, Kyung Hee University) ;
  • Sohn Nak-Won (Department of Neuroscience, Graduate School of East-West Medical Science, Kyung Hee University)
  • 정춘근 (경희대학교 한의과대학 해부학교실) ;
  • 김은영 (경희대학교 한의과대학 해부학교실) ;
  • 신정원 (경희대학교 동서의학대학원 신경과학교실) ;
  • 손영주 (상지대학교 한의과대학 부인과학교실) ;
  • 이현삼 (경희대학교 한의과대학 해부학교실) ;
  • 정혁상 (경희대학교 한의과대학 해부학교실) ;
  • 손낙원 (경희대학교 동서의학대학원 신경과학교실)
  • Published : 2005.06.01

Abstract

Objectives: Chungpaesagan-tang (CPSGT), which is frequently used for treating patients of cerebrovascular disease, has not been reported by clinical doctors concerning the effect of neuronal aptosis caused by brain ischemia. To study the effect of CPSGT on focal cerebral ischemia in normal and diabetic rats and SHR, focal cerebral ischemia was induced by transient MCAO, and after onset CPSGT was administrated. Methods: Rats (Sprague-Dawley) were divided into four groups: sham-operated group, MCA-occluded group, CPSGT­administrated group after MCA occlusion, and normal group. The MCA was occluded by intraluminal method. CPSGT was administrated orally twice (l and 4 hours) after middle cerebral artery occlusion. All groups were sacrificed at 24 hours after the surgery. The brain tissue Was stained with $2\%$ triphenyl tetrazolium chloride (TTC) or $1\%$ cresyl violet solution, to examine effect of CPSGT on ischemic brain tissue. The blood samples were obtained from the heart.~. Tumor necrosis $factor-\alpha$ level and interleukin-6 level of serum was measured from sera using enzyme-linked immunoabsorbent assay (ELISA). Then changes of immunohistochemical expression of $TNF-\alpha$ in ischemic damaged areas were observed. Results: In NC+MCAO+CP and DM+MCAO+CP, CPSGT significantly (p<0.01) decreased the number of neuron cells compared to the control group. CPSGT markedly reduced (p<0.01) the infarct size of the forebrain in distance from the interaural line on cerebral ischemia in diabetic rats. CPSGT significantly reduced the $TNF-\alpha$ expression in penumbra region of damaged hemisphere in diabetic rats. Conclusions: CPSGT had a protective effect on cerebral ischemia in SD rats, especially in diabetic rats compared with normal SD rats.

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