Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Hepatic Tissue Changes by the 1,3-Dichloropropanol Inhalation in the Rat

1, 3-Dichloropropanol 흡입에 의한 랫드간의 조직변화에 관한 연구

  • Kim Sung-Hwa (College of the Veterinary Medicine Chungnam National University) ;
  • Park O-Sung (College of the Veterinary Medicine Chungnam National University) ;
  • Lee Sung-Bae (Occupational Safety & Health Research Institute Center For Occupational Toxicology) ;
  • Choi Jong-Yun (College of the Veterinary Medicine Chungnam National University) ;
  • Kwon Hyo Jung (College of the Veterinary Medicine Chungnam National University) ;
  • Son Sek-Woo (College of the Veterinary Medicine Chungnam National University) ;
  • Park Il-Kwon (College of the Veterinary Medicine Chungnam National University) ;
  • Lee Kyoung-Youl (College of the Veterinary Medicine Chungnam National University) ;
  • Son Hwa-Young (College of the Veterinary Medicine Chungnam National University) ;
  • Lee Mee-Young (College of the Veterinary Medicine Chungnam National University) ;
  • Lee Guen-Jwa (College of the Veterinary Medicine Chungnam National University) ;
  • Kim Hyeon-Young (Occupational Safety & Health Research Institute Center For Occupational Toxicology) ;
  • Lee Kang-Yi (College of Oriental Medicine, Daejeon University)
  • 김성화 (충남대학교 수의과대학) ;
  • 박오성 (충남대학교 수의과대학) ;
  • 이성배 (산업안전보건연구원 산업화학물질연구센터) ;
  • 최종윤 (충남대학교 수의과대학) ;
  • 권효정 (충남대학교 수의과대학) ;
  • 손석우 (충남대학교 수의과대학) ;
  • 박일권 (충남대학교 수의과대학) ;
  • 이경열 (충남대학교 수의과대학) ;
  • 손화영 (충남대학교 수의과대학) ;
  • 이미영 (충남대학교 수의과대학) ;
  • 이근좌 (충남대학교 수의과대학) ;
  • 김현영 (산업안전보건연구원 산업화학물질연구센터) ;
  • 이강이 (대전대학교 한의과대학)
  • Published : 2005.06.01
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Abstract

1,3-Dichloro-2-propanol (1,3-DCP) is known as chloride chemicals and causes severe hepatotoxic agent. The Ito cells and Kupffer's cells of the liver in the 5 old F344 Rats were exposed to 1,3-DCP gas chamber for 6 hours/ a day, 5 days/ a week, and 13 weeks, in the 0, 5, 20, 80 ppm, respectively. After then the body weights, liver weights, and relative liver weight to body weight were measured, and the hepatic tissues were prepared by the routine and Immunostain method, and observed by the LM, and EM. In the results, there were severe body weight decrease (p<0.05) in the 80 ppm of the male and female rats. The relative liver weights to the body weight were increased relate with exposed 1,3-DCP concentration (P<0.001). Inflammatory cells, infiltration was observed at the perivascular area in the 20 ppm exposed group, and bilirubin pigment infiltration, bile duct hyperplasia, inflammation hepatocytic necrosis, fibrosis were observed in the 80 ppm exposure group. In the 80 ppm exposure group, disarrangement of the endothelial cells, erythrocytes and hepatic cell fragment in the Disse space and numerous migration macrophages were observed in the necrotic area by EM observation. In the immunostained hepatic tissues positive stained ED1 cells were extremely increased (P<0.05) in central vein area, but ED2 was weakly positive immunostained in the 80 ppm exposed group. Immunostained desmin was observed in the Ito cell. It was no difference in the low and medium exposed group but it was typical increase in the necrotic area. In conclusion, These results suggest that NOAEL of 1,3-DCP may be 5 ppm in rats and the Immunostained of desmin, ED1 and ED2 positive cells activated in the inflammatory liver were related to the exposure volume and density. The increase of the Ito cells were related to the severe phagocytosis of the Kupffer's cells.

Keywords

References

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