Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
권호 표지

Production of Plasminogen Activators during In Vitro Maturation of Fresh or Frozen- Thawed Oocytes in the Pig

  • Chen J. B. (College of Animal Resources Science, Kangwon National University) ;
  • Sa S. J. (College of Animal Resources Science, Kangwon National University) ;
  • Cao Y. (College of Bioengineering, Dalian University) ;
  • Choi S. H. (Animal Genetic Resources Station, National Livestock Research Institute) ;
  • Cheong H. T. (College of Animal Resources Science, Kangwon National University) ;
  • Yang B. K. (College of Animal Resources Science, Kangwon National University) ;
  • Park C. K. (Division of Animal Resourcem Kangwon National University)
  • Published : 2005.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study were examined whether plasminogen activators (PAs) are produced by porcine fresh or frozen-thawed cumulus-oocytes complexes (COCs) and cumulus cell free-oocytes. In fresh or frozen-thawed COCs and oocytes for 0 hour cultured, no activity of PAs was detected. However, at 24 hours of culture urokinase-type plasminogen activator (uPA) was detected in COCs and denuded oocytes. In the frozen-thawed COCs and cumulus cell free-oocytes cultured for 24 hours, no PAs were observed. After COCs were cultured for 48 hours, tissue-type plasminogen activator (tPA) and tPA-PAI were observed in COCs only. In the frozen-thawed COCs and cumulus cell free-oocytes cultured for 48 hours, no PAs were observed. These results suggest that uPA, tPA and tPA-PAI are produced by porcine COCs, but only uPA by oocytes during maturation for 24 hours. Only tPA, and tPA-PAI are produced by COCs cultured for 48 hours, and no PAs are produced by denuded-oocytes cultured for 48 hours. In all of the frozen-thawed groups, no PAs are observed by COCs and denuded-oocytes.

Keywords

References

  1. Al-Hasani S, Diedrich K, Van H, Reinecke A, Hartje M, Krebs D (1987): Cryopreservation of human oocytes. Hum Reprod 2:695-700 https://doi.org/10.1093/oxfordjournals.humrep.a136616
  2. Almeida PA, Bolton VN (1995): The effect of temperature fluctuations on the cytoskeletal organization and chromosomal constitution of the human oocytes. Zygote 3:357-365 https://doi.org/10.1017/S0967199400002793
  3. Aman RR, Parks JE (1994): Effects of cooling and rewarming on the meiotic spindle and chromosomes of in vitro-matured bovine oocytes. Biol Reprod 50:103-110 https://doi.org/10.1095/biolreprod50.1.103
  4. Arav A, Zeron Y (1997): Vitrification of bovine oocytes using modified minimum drop size technique (MDS) is effected by the composition and concentration of the vitrification solution and by the cooling conditions. Theriogenology 47:341 https://doi.org/10.1016/S0093-691X(97)82468-5
  5. Beers WH, Strickland S, Reich E (1975): Ovarian plasminogen activator: relationship to ovulation and hormonal regulation. Cell 6:387-394 https://doi.org/10.1016/0092-8674(75)90188-9
  6. Berg DA, Menino AR (1992): Bovine embryos produce a uroknase-type plasminogen activator. Mol Reprod Dev 31:14-19 https://doi.org/10.1002/mrd.1080310104
  7. Bicsak TS, Cajander SB, Peng XR, LaPolt PS, Lu JKH, Krisensen P, Tsafriri A, Hsueh AJW (1989): Tissue-type plasminogen activator in rat oocytes: expression during the periovulatory period, agter fertilization, and during follicular atresia. Endocrnology 124:187-194 https://doi.org/10.1210/endo-124-1-187
  8. Cooper A, O'Neil L, Bernard A, Fuller B, Shaw R (1996): The effect of protein source and temperature on the damage to mouse oocytes cytoskeleton after exposure to a vitrification solution. Cryo Lett. 17:149-156
  9. Dyk A, Menino AR (1991): Electrophoretic characterization of the plasminogen activator produced by bovine blastocysts. J. Reprod Fertil 93:483-489 https://doi.org/10.1530/jrf.0.0930483
  10. Fazleabas AT, Geisert RD, Bazer FW (1983): Relationship between release of plasminogen activator and estrogen by blastocysts and secretion of plasmin inhibitor by uterine endometrum in the pregnant pig. Biol Reprod 29:225-238 https://doi.org/10.1095/biolreprod29.1.225
  11. George M, Johnson MH (1993): Cytoskeletal organization and zona sensitivity to digestion by chymotrypsin of frozen-thawed mouse oocytes. Hum Reprod 7:612-620
  12. Gwatkin RBL, William DT, Hartmann JF, Kniazuk M (1973): The zona reaction of hamster and mouse eggs: production in vitro by a trypsin-like protease from cortical granules. J Reprod Fertil 32:259-265 https://doi.org/10.1530/jrf.0.0320259
  13. Hamano S, Koikeda A, Kuwayama M, Nagai T (1992): Full-term development of in vitro matured and fertilized bovine oocytes. Theriogenology 38:1085-1090 https://doi.org/10.1016/0093-691X(92)90122-8
  14. Hochi S, Ito K, Hirabayashi M, Ueda M, Kimura K, Hanada A (1998): Effect of nuclear stages during IVM on the survival of vitrified-warmed bovine oocytes. Theriogenology 49:787-796 https://doi.org/10.1016/S0093-691X(98)00028-4
  15. Huarte J, Belin D, Vassalli JD (1985): Plasminogen activator in mouse and rat oocytes: induction during meiotic maturation. Cell 43:551-558 https://doi.org/10.1016/0092-8674(85)90184-9
  16. Huseh AJW, Liu YX, Cajander S, Peng XR, Dahl K, Kristensen P, Ny T (1988): Gonadotropin-releasing hormone induces ovulation in hypophysectomized rats: studies on ovarian tissue-type plasminogen activator activity, messenger ribonucleic and content, and cellular localization. Endocrinology 122:1486-1495 https://doi.org/10.1210/endo-122-4-1486
  17. Hyttel P, Vajta G, Callesen H (2000): Vitrification of bovine oocytes with open pulled straw method: ultra-structural consequences. Mol Reprod Dev 56:80-88 https://doi.org/10.1002/(SICI)1098-2795(200005)56:1<80::AID-MRD10>3.0.CO;2-U
  18. Kaaekuahiwi MA, Menino AR (1990): Relationship between plaminogen activator production and bovine embryo development in vitro. J Anim Sci 68:2009-2014 https://doi.org/10.2527/1990.6872009x
  19. Kim NH, Menino AR (1995): Effects of protein kinase A and C and modulators of phosphorylation and plasminogen activator activity in procine oocyte-cumulus cells complexes during in vitro maturation. Mol Reprod Dev 40:364-370 https://doi.org/10.1002/mrd.1080400313
  20. Le GF, Massip A (1999): Cryopreservation of cattle oocytes: effect of meiotic stage, cyclohexamide treatment, and vitrification. Cryobiology 38:290-300 https://doi.org/10.1006/cryo.1999.2172
  21. Liedholm P, Astedt B (1975): Fibrinolytic activity of the rat ovum, appearance during tubal passage and disappearance at implantation. Int J Fertil. 20:24-26
  22. Lim JM, Fukui Y, Ono H (1991a): Developmental competence of bovine oocytes frozen at various maturation stages followed by in vitro maturation and fertilization. Theriogenology 37:351-361 https://doi.org/10.1016/0093-691X(92)90193-U
  23. Lim JM, Fukui Y, Ono H (1991b): The post-thaw developmental capacity of frozen bovine oocytes following in vitro maturation and fertilization. Theriogenology 35:1225-1235 https://doi.org/10.1016/0093-691X(91)90368-N
  24. Liu YX, Ny T, Sarkar D, LoskutoffD, Hsueh AJW (1986): Identification and regulation of tissue plasminogen activator in rat cumulus-oocyte complexes. Endocrinology 119:1578-1587 https://doi.org/10.1210/endo-119-4-1578
  25. Liu YX, Cajande SB, Ny T, Kristen P, Hsueh AJW (1987): Gonadotropin regulation of tissue-type and urokinase-type plasminogen activators in rat granulosa and theca-interstitial cells during the periovulatory period. Mol Cell Endocrinol 54:221 https://doi.org/10.1016/0303-7207(87)90160-2
  26. Liu RH, Sun QY, Li YH, Jiao LH, Wang WH (2003): Effects of cooling on meiotic spindle structure and chromosome alignment within in vitro matured porcine oocytes. Mol Reprod Dev 65:212-218 https://doi.org/10.1002/mrd.10282
  27. Magistrini M, Szollosi D (1980): Effects of cold and of isopropyl-N-phenylcarbamate on the second meiotic spindle of mouse oocytes. Eur J Cell Biol 22:699-707
  28. Martino A, Pollard JW, Leibo SP (1996a): Effect of chilling bovine oocytes on their developmental competence. Mol Reprod Dev 45:503-512 https://doi.org/10.1002/(SICI)1098-2795(199612)45:4<503::AID-MRD13>3.0.CO;2-X
  29. Men H, Monson RL, Rutledge JJ (2002): Effect of meiotic stage and maturation protocols on bovine oocytes resistance to cryopreservation. Theriogenology 57:1095-1103 https://doi.org/10.1016/S0093-691X(01)00679-3
  30. Menino AR, Williams JS (1987): Activation of plasminogen by the early bovine embryo. Biol Reprod 36:1289-1295 https://doi.org/10.1095/biolreprod36.5.1289
  31. Ny T, Liu YX, Ohlsson M, Jones PBC, Hsueh AJW (1987): Regulation of tissue-type plaminogen activator activity and messenger RNA levels by gonadotropin-releasing hormone in cultured rat granulosa cells and cumulus-oocyte complexes. J Biol Chem 262:11790-11793
  32. Petters RM, Wells KD (1993): Culture of pig embryos. Journal of Reproduction and Fertility Supplement, 48:61-73
  33. Pickering SJ, Braude PR, Johnson MH, Cant A, Currie J (1990): Transient cooling to room temperature can cause irreversible disruption of the meiotic spindle in the human oocytes. Fertil Steril 54:102-108 https://doi.org/10.1016/S0015-0282(16)53644-9
  34. Rho GJ, Kim S, Yoo JG, Balasubramanian S, Lee HJ, Choe SY (2002): Microtubulin configuration and mitochondrial distribution after ultra-rapid cooling of bovine oocytes. Mol Reprod Dev 64:464-470
  35. Richardson RR, Parks JE (1992): Effects of chilling on the meiotic spindle and chromosomes of bovine ova. Theriogenology 37:284(abstract) https://doi.org/10.1016/0093-691X(92)90353-S
  36. Salustri A, Petrungaro S, De FM, Conti M, Siracusa G (1985): Effect of follicle-stimulating-hormone on cyclic adenosine monophosphate level and on meiotic maturation in mouse cumulus cell-enclosed oocytes cultured in vitro. Biol Reprod 33:797-802 https://doi.org/10.1095/biolreprod33.4.797
  37. Sathananthan AH, Trounson A, Freeman L, Brady T (1988): The effects of cooling human oocytes. Hum Reprod 3:968-977 https://doi.org/10.1093/oxfordjournals.humrep.a136827
  38. Saunders K, Parks JE (1999): Effects of cryopreservation procedures on the cytology and fertilization rate of in vitro matured bovine oocytes. Biol Reprod 61:178-187 https://doi.org/10.1095/biolreprod61.1.178
  39. Shamsuddin M, Niwa K, Larsson B, Rodriguez MH (1996): In vitro maturation and fertilization of bovine oocytes. Reprod Dom Anim 31:613-622
  40. Sherman MI (1980): Studies on the temporal correlation between secretion of plasminogen activator and stags of early mouse embryogenesis. Oncodev Biol Med 1:7-17
  41. Son WY, Park SE, Lee KA, Lee WS, Ko JJ, Yoon TK (1996): Effects of 1,2-propanediol and freezing-thawing on the in vitro developmental capacity of human immature oocytes. Fertil Steril 66:995-999 https://doi.org/10.1016/S0015-0282(16)58696-8
  42. Strickland S, Reich E, Sherman MI (1976): Plasminogogen activator in early embryogenesis: enzyme production by trophoblast and parietal endoderm. Cell 9: 231-240 https://doi.org/10.1016/0092-8674(76)90114-8
  43. Vajta G, Holm P, Kuwayama M, Booth PJ, Jacobsen H, Greve T (1998a): Open Pulled Straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol Reprod Dev 51:53-58 https://doi.org/10.1002/(SICI)1098-2795(199809)51:1<53::AID-MRD6>3.0.CO;2-V
  44. Vajta G, Lewis IM, Kuwayama M, Greve T, Callesen H (1998b): Srerile application of the open pulled straw (OPS) vitrification method. Cryo Letters 19:389-392
  45. Vincent C, Garnier V, Heyman Y, Renard JP (1989): Solvent effects on cytoskeletal organization and in-vivo survival after freezing of rabbit oocytes. J Reprod Fertil 87:809-820 https://doi.org/10.1530/jrf.0.0870809
  46. Vincent C, Johnson M (1992): Cooling, Cryoprotectants and the cytoskeleton of the mammalian oocytes. In; Milligan SR(Ed.). Oxford reviews of reproductive biology, vol. 14. Oxford University Press. pp:73-100
  47. Wu B, Tong J, Leibo SP (1999): Effects of cooling germinal vesicle-stage bovine oocytes on meiotic spindle formation following in vitro fertilization. Mol Reprod Dev 54:388-395 https://doi.org/10.1002/(SICI)1098-2795(199912)54:4<388::AID-MRD9>3.0.CO;2-7