Screening of Fibrinolytic Activities from Cultured Mushrooms

인공재배 버섯의 혈전분해효소 활성검색

  • Choi, Han-Seok (Department of Food Science and Technology, Chonbuk National University) ;
  • Kim, Myung-Kon (Department of Industrial Crop Production and Processing, Iksan National College) ;
  • Park, Hyo-Suk (Department of Agricultural Chemistry, Wonkwang University) ;
  • Kim, Jae-Sung (Department of Genetic Engineering, Chosun University) ;
  • Shen, Ming-Hva (Department of Genetic Engineering, Chosun University) ;
  • Kim, Sung-Jun (Department of Genetic Engineering, Chosun University)
  • Published : 2005.12.31

Abstract

Fibrinolytic activities of cultured medium and mycelium from 13 selected culturable mushrooms ranged 0.00-0.16 U and 0.00-0.48 U, respectively. Dry cell weight of Armillaria mellea was 6.50g/100mL, 1.9-5.0 fold higher than other tested mushrooms. Among artificially cultured mushroom fruiting bodies tested, fibrinolytic activity was lowest in Hericium erinaceus (0.17 U) and highest in Pleurotus ferulea (0.94 U).

인공재배 가능한 버섯 13종을 선정하여 균사체 배양 후 배양액 및 혈전분해활성을 검토한 결과 배양액에서는 0.00-0.16U 매우 낮았던 반면에 균사체의 혈전분해 활성은 0.00-0.48U로 배양액에 비하여 상대적으로 높은 활성을 보였다. 또한, 뽕나무 버섯의 건조 균체량은 6.50g/100mL로 다른 균체에 비하여 1.9-5.0배의 높은 생산력을 보여주었다. 인공 재배된 버섯 자실체(7종)의 혈전분해활성은 0.17-0.94U로 나타났으며 아위버섯 자실체의 혈전분해 활성이 가장 높게 나타났다.

Keywords

References

  1. Kim MK, Kim HM, Nha ES, Yoo SH, Chai JK, Hong JS. Mushroom biology. Hakmunsa, Seoul, Korea. p. 10 (2002)
  2. Lee JY. Additional list of recorded mushrooms in Korea (from 2001 to 2004). Korean J. Micol. 33: 54-57 (2005) https://doi.org/10.4489/KJM.2005.33.1.054
  3. Kim JH, Yoo KH, Seok SJ. Kim YS. Screening of fibrinolytic activities of extracts from wild mushrooms collected in Mt. Chilgap of Korea. Korean J. Micol. 33: 18-21 (2005) https://doi.org/10.4489/KJM.2005.33.1.018
  4. Chang ST, Miles PG. Mushroom science. pp. 3-25. In: Edible mushroom and their cultivation. CRC press, Inc., Boca Raton, FL, USA. (1989)
  5. Kawagishi H, Shimada A, Mori H, Okamoto KF, Sakamoto H, Furukawa S. Erinacines D, a stimulator of nerve growth factor (NGF)-synthesis, from the mycelia of Hercium erinaceum. Heterocyclic Comm. 2: 51-54 (1996)
  6. Mizuno T. Yamabushitake, Hericium erinaceum: bioactive substances and medical utilization. Food Rev. Int. 11: 173-178 (1995) https://doi.org/10.1080/87559129509541027
  7. Choi HS, Kim MK, Park HS, Kim JS, Kim SJ. Effect of various plants extracts on the mycelial growth and fibrinolytic activity of Arimillaria mellea. Korean J. Micol. 33: 11-17 (2005) https://doi.org/10.4489/KJM.2005.33.1.011
  8. Choi NS, Seo SY, Kim SH. Screening of mushrooms having fibrinolytic activity. Korean J. Food Sci. Technol. 31: 553-557 (1999)
  9. Reed GL, Lin LF, Parhaml-Seren B, Kussie P. Identification of plasminogen binding region in streptokinase that is necessary for the creation of streptokinase-plasminogen activator complex. Biochem. 34: 10266-10271 (1995) https://doi.org/10.1021/bi00032a021
  10. Mihara H, Sumi H, Yoneta T, Mizumoto H, Ikeda R, Seiki M, Maruyama M. A novel fibrinolytic enzyme extracted from the earthworm. Lumbricus fubellus. Jpn. J. Physiol. 41: 461-468 (1991) https://doi.org/10.2170/jjphysiol.41.461
  11. Chung KH, Kim DS. Fibrinolytic and coagulation activities of Korean snake venoms. J. Korean Biochem. 25: 696-701 (1992)
  12. Fujita M, Nomura K, Hong K, Ito Y, Asada A, Nishimuro S. Purification and characterization of a strong fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto, a popular soybean fermented food in Japan. Biochem. Biophys. Res. Comm. 197: 1340-1347 (1993) https://doi.org/10.1006/bbrc.1993.2624
  13. Sumi H, Nakajima N, Yatagai C. A unique strong fibrinolytic enzyme (katsuwokinase) in skipjack 'Shiokara' a Japanese traditional fermented food. Comp. Biochem. Pytosiol. 112B: 543-547 (1995)
  14. Kim JS, Lee HY, Yoo KH, Kim YS, Seok SJ, Kim YS. The screening of fibrinolytic activities of extracts from mushrooms in Mt. Chiak. Korean J. Mycol. 26: 589-593 (1998)
  15. Healy V, O'Connell J, McCarthy TV, Doonan S. The lysine-specific proteinase from Armillaria mellea is a member of a novel class of metalloendopeptidases located in basidiomycetes. Biochem. Biophys. Res. Comm. 262: 60-63 (1999) https://doi.org/10.1006/bbrc.1999.1151
  16. Kim MK, Choi HS, Park HS, Kim SJ. Optimal condition for mycelial production of Armillaria mellea. Korean J. Micol. 31: 187-191 (2003) https://doi.org/10.4489/KJM.2003.31.3.187
  17. Lee SY, Kim JS, Kim JE, Sapkota K, Shen MH, Kim S, Chun HS, Yoo JC. Choi HS. Kim MK, Kim SJ. Purification and characterization of fibrinolytic enzyme from cultured mycelia of Armillaria mellea. Protein Express. Purif. 43: 10-17 (2005) https://doi.org/10.1016/j.pep.2005.05.004
  18. Hong, KH, Kim BY, Kim HK. Analysis of nutritional components in Pleurotus ferulea. Korean J. Food Sci. Technol. 36: 563-567 (2004)
  19. Kim JS, Kim YS. Purification and characterization of fibrinolytic enzyme from Armillaria mellea. Korean J. Micol. 26: 583-588 (1998)