Journal of the Korean Society of Food Culture (한국식생활문화학회지)

Korean Society of Food Culture (한국식생활문화학회)
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Physicochemical Characteristics of Yogurt Prepared with Lactic Acid Bacteria Isolated from Kimchi

김치 유래의 내산성 유산균으로 제조한 요구르트의 이화학적 특성

  • Kim, Seon-Jae (Food Industrial Technology Research Center, Mokpo National University)
  • 김선재 (목포대학교 식품산업기술연구센터(RRC))
  • Published : 2005.06.30
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Abstract

Fourteen strains out of fifty six strains of lactic acid bacteria isolated from Kimchi showed a resistance to artificial gastric juice. In particular, lactobacilli AK 3, AK 7, BK 28, and DK 37 showed a strong resistance and their viable cell counts of the initial stage were no change after the 2 hours cultivation in an artificial gastric juice. All five lactic acid bacteria were used as starters in producing yogurts. The physicochemical characteristics of yogurts were examined. The original pH, titratable acidity, visicosity and viable cell counts of yogurts were $3.78{\sim}4.32,\;0.96{\sim}1.41%,\;1,659{\sim}2,348\;cps\;and\;1.1{\times}10^9{\sim}2.1{\times}10^9cfu/mL$, respectively. The ${\beta}-galactosidase$ activity reached maximum at 48 hr, and reduced gradually during incubation.

전남 목포의 각 가정에서 담근 김치로부터 분리된 총 56균주의 유산균 중 14균주가 pH 2.5인 인공위액에 내성을 나타냈다. 이 내산성 유산균은 인공위액에서 2시간 배양할 경우, 초기 유산균수의 수 $10^9/mL$에서 유산균수가 $10^7{\sim}10^9/mL$의 범위를 나타냈다. 김치로부터 분리된 내산성 유산균 AK 3, AK 7, BK 28 그리고 DK 37균주들은 초기 생균수에 비해 인공위액에서 2시간 진탕 후의 생균수가 감소하였지만, 그 생존율이 59-77%를 나타나 다른 유산균에 비해 인공위액에 대해 비교적 높은 내성을 나타냈다. 내산성 유산균으로 배양한 요구르트 5종류는 전체적으로 pH는 $3.78{\sim}4.32$ 사이였으며, 산도는 $0.96{\sim}1.41%$, 그리고 점도는 $1,659{\sim}2,348cps$로 나타났다. 요구르트의 유산균 생균수는 $1.1{\times}10^9{\sim}2.1{\times}10^9\;cfu/mL$의 범위를 나타냈다. 내산성 유산균 BK 28이 다른 균주에 비해 pH 3.78, 산도는 1.41%를 나타내었고 점도가 2,348 cps로 가장 높게 나타났다. 산성 유산균의 ${\beta}-galactosidase$ 활성은 경시적으로 효소활성이 증가하는 경향을 나타냈으며, 48시간에 최고의 활성도를 나타냈지만 그 이후에는 감소하는 경향을 나타냈다.

Keywords

References

  1. Crit. Rev Food Sci Nut v.34 Biochemical, microbiological, and nutritional aspects of Kimchi Cheigh, H.C.;Park, K.Y.
  2. Kor J Microbiol Biotechno v.30 Characteristics of the bacteriocin from Lactobacillus sp. Oh-B3 Kim, D.S.
  3. Kor J Microbiol Biotechnol v.31 Diversity and antibacterial activity of lactic acid bacteria isolated from Kimch Ahn, D.K.;Han, T.W.;Shin, H.Y.;Jin, I.N.;Ghim, S.Y.
  4. Can J Microbiol v.39 Detection and characterization of a bacteriocin produced by Lactobacillus plantarum C19 Atrih, A.;Rekhif, N.;Milliere, J.B.;Lefebvre, G.
  5. Appl Environ Microbiol v.59 The assumed assimilation of cholesterol Lactobacilli and Bifidobacterium bifidium is due to their bile saltconjugating activity Klaver, F.A.M.;van der Meer, R.
  6. J Microbiol v.39 Isolation of dextranproducing Leuconostoc lactis from Kimchi Kim, B.J.;Min, B.H.;Kim, J.H.;Han, H.U.
  7. J Microbiol Biotechnol v.12 Bacteriocin produced by Pediococcus sp. in Kimchi and its characteristics Kwon, D.Y.;Koo, M.S.;Ryoo, C.R.;Kang, C.H.;Min, K.H.;Kim, W.J.
  8. Kor J Microbiol Biotechnol v.27 Isolation and characterization of lactic acid bacteria producing antimutagenic substance Korean Kimchi Rhee, C.H.;Park, H.D.
  9. J Appl Microbiol v.94 Probiotic properties of human lactobacilli strains to be used in the gastrointestinal tract Fernandez, M.F.;Boris, S.;Barbes, C.
  10. Lett Appl Microbiol v.32 Partial characterization of polyfermenticin SCD, a newly identified bacteriocin of Bacillus polyfermenticus Lee, C.H.;Jun, K.D.;Kim, W.S.;Paik, H.D.
  11. Brit J Surg v.88 Probiotics OSullivan, G.C.
  12. J. Food Sic v.46 Rapid purification of ${\beta}-galactosidase$(Aspergillus niger) from a commercial preparation Greenberg, N.A.;Mahoney, R.R.
  13. J Amer Chem Soc v.80 Structural characterization of products of enzymatic disproportionation oflactose Pazur, J.H.;Tipton, T.M.;Budovich, J.;Marsh, J.M.
  14. Kor J Microbiol Biotechnol v.31 Some probiotic properties of some lactic acid bacteria yeasts isolated from Jeot-gal Lee, N.K.;Kim, H.W.;Cho, S.Y.;Paik, H.D.
  15. Jpn J Microbiol v.29 Tolerance of the multiple antibiotic resistant strain, L. casei PSR 3002, to artificial digestive fluids Kobayashi, Y.;Tohyama, K.;Terashima, T.
  16. J Food Sci v.56 Production and evaluation of milk-sweet potato mictures fermented with yogurt bacteria Collins, J.L.;Ebah, C.B.;Mount, J.R.;Demott, B.J.;Draughon, F.A.
  17. J Food Sci v.55 Survival of lactic acid bacteria and their lactases under acidic conditions Shan, N.;Jelen, P.
  18. Am J Clin Nutr v.45 Lactose digestion by yogurt ${\beta}$-galactosidase : influence of pH and microbial cell integrity Martini, M.;Bollweg, C.G.L.;Levitt, M.D.;Savaiano, D.A.
  19. Food Sci Biotechnol v.13 Identification and probiotic properties of Lactocossus latis NK24 isolated from Jept-gal, a Korean fermented food Lee, N.K.;Kim, T.H.;Choi, S.Y.;Lee, S.K.;Paik, H.D.
  20. Agr Chem Biotech v.37 Survival rate of lactic acid bacteria and the change of ${\beta}$-galactosidase activity in commercial yogurts under the acidic conditions Shin, Y.S.;Sung, H.J.;Kim, D.H.;Lee, K.S.
  21. Agr Chem Biotech v.39 Phsicochemical characteristics and ${\beta}$-galactosidase activity of Lactobacillus plantarum from Kimchi Rhee, Y.H.;Kang, M.S.