Archives of Reconstructive Microsurgery

The Korean Society for Microsurgery (대한미세수술학회)
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Effect of ${\alpha}-Lipoic$ Acid on Expression of pERK1/2 following Ischemia-Reperfusion Injury in the Hindlimb Muscle Flap of Rats

흰쥐 후지근 피판에서 허혈-재순환 손상시 pERK1/2 발현에 대한 ${\alpha}-lipoic$ Acid의 효과

  • Song, Jeong-Hoon (Department of Plastic & Reconstructive Surgery, School of Medicine, Wonkwang University) ;
  • Kim, Min-Sun (Department of Physiology, School of Medicine, Wonkwang University) ;
  • Park, Byung-Rim (Department of Physiology, School of Medicine, Wonkwang University) ;
  • Park, Han-Su (Department of Physical Education, Kun San University) ;
  • Chae, Jeong-Ryong (Department of Physical Education, Kun San University) ;
  • Lee, Hye-Me (Department of Plastic & Reconstructive Surgery, School of Medicine, Wonkwang University) ;
  • Na, Young-Cheon (Department of Plastic & Reconstructive Surgery, School of Medicine, Wonkwang University)
  • 송정훈 (원광대학교 의과대학 성형외과학교실) ;
  • 김민선 (원광대학교 의과대학 생리학교실) ;
  • 박병림 (원광대학교 의과대학 생리학교실) ;
  • 박한수 (군산대학교 체육학과) ;
  • 채정룡 (군산대학교 체육학과) ;
  • 이혜미 (원광대학교 의과대학 성형외과학교실) ;
  • 나영천 (원광대학교 의과대학 성형외과학교실)
  • Published : 2005.10.31
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Abstract

Purpose: This study was to evaluate the effect of ${\alpha}-lipoic$ acid, a potent free radical scavenger, on the expression of active form of extracellular signal-regulated kinase (pERK1/2) proteins from hindlimb muscles of rats following ischemia-reperfusion injury. Material and methods: 64 health, $280{\sim}350\;g$ weighted Sprague-Dawley male rats were used. In order to make a muscle flap, the gastrocnemius (GC) and soleus (SOL) muscles were dissected and elevated. The popliteal artery was occluded for 4hours and reperfused for 10 minutes, 30 minutes, 1 hour, 2 hours and 4 hours, respectively. Results: The ischemia by occlusion of the popliteal artery itself caused a minimal change in expression of phosphorylated form of proteins observed in hindlimb muscle. In contrast, after 4 hours of ischemia, immunoreactivity for pERK1/2 in the GC muscle showed dual peaks at 10 minutes and 4 hours after reperfusion. In ${\alpha}-lipoic$ acid treated group, the expression of pERK1/2 was increased significantly compared to I/R-only group. Conclusion: These results suggest that ${\alpha}-lipoic$ acid may protect I/R injury of the skeletal muscle through free radical scavening and activation of intracellular pERK1/2 expression.

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