Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
권호 표지

THE CHANGES OF SALIVARY GLAND AFTER THE LIGATION OF THE EXCRETORY DUCT IN SUBMANDIBULAR GLANDS

악하선 분비관의 결찰 후 타액선 조직의 변화

  • Cha, Seung-Man (Department of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University) ;
  • Chung, In-Kyo (Department of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University)
  • 차승만 (부산대학교 치과대학 구강악안면외과학교실) ;
  • 정인교 (부산대학교 치과대학 구강악안면외과학교실)
  • Published : 2005.10.30
Download PDF
⟨ Previous Next ⟩

Abstract

Obstructive adenitis of the salivary gland following salivary stone or infectious disease of the gland and surrounding tissues is a common disease. It is often difficult to decide whether to perform total excision of the gland or to consider conservative treatment. The present study was designed to investigate histological, histochemical changes of submandibular gland after ligating the excretory duct for identifying the results of gland duct obstruction. A group of 40 rat of Sprague-Dawley weighing about $200{\sim}220gm$ were used in the present study. 30 rats had ligation of the main excretory ducts of submandibular glands just at the exit from the glands. For controls, 10 rats had a sham operation without duct ligation. They were inducted into euthanagia state by intracardial Ketamine injection in 1, 2, 3, 5, 7, 14, 21, 28, 35 and 42 days after the ligation. In each ligation period, 3 animals were used for ligation and one animal was for control. The submandibular glands were dissected out at sacrifice and stained with H&E, PAS, mucicarmine stain and histological examination were carried out under the light microscope. After examination and comparison of all specimens, the results were as follows: 1. In the features of H&E stain, acini disappeared by degrees after the ligation of the excretary duct and interstitial cells were displaced into fibrous connective tissue. Salivary gland had been atrophied with enlarging ducts and proliferating ductal cells. 2. Through total experimental period, a lot of vessels were observed and the atrophy of serous gland was severer than that of mucous gland. 3. The deep portion of submandibular glands showed severe degeneration rather than superficial portion of them after the ligation. 4. The changes which had enlarged ducts and proliferating ductal cells were observed in entire gland and more prominent in serous gland than mucous gland after the ligation. 5. Although PAS and mucicarmine reactions were decreased gradually after the ligation with the lapse of time, since 2 to 3 weeks they were strong positive reactions on entire gland, especially on duct-like structure. So, we can suggest that salivary gland will be atrophied but, survived acini will be redistributed around the ducts.

Keywords

References

  1. A.R. Ren Cate: Oral Histology. Development, Structure, and Function. 4th Ed. 356-388, The C.V. Mosby Company Saint Louic., 1994
  2. Hoover WB: Obstructions of salivary ducts. Surg Clin North Am 1931;11:303-313
  3. Baron HC, Ober WB: Parotid gland atrophy. Arch Surg 1962; 85:1042-1044 https://doi.org/10.1001/archsurg.1962.01310060178034
  4. Elmostehy MR: Parotid salivary calculus. Oral Surg 1968;26:18-21 https://doi.org/10.1016/0030-4220(68)90216-8
  5. Bhasker SN, Bolden TE, Weinmann JP: Experimental obstructive adenitis in the mouse. J Dent Res 1956;35:852-862 https://doi.org/10.1177/00220345560350060501
  6. Batsakis JG, Regezi JA, Luna MA, Naggar A: Histogenesis of salivary gland neoplasm: a postulate with prognostic implications. J Laryngol Otol 1989;103:939-944 https://doi.org/10.1017/S0022215100110552
  7. Regezi JA, Batsakis JG: Histogenesis of salivary gland neoplasms. Otolaryngol Clin North Am 1977;10:297-307
  8. Tamarin A: Submaxillary gland recovery from obstruction: I. Overall changes and electron microscopic alterations of granular duct cells. J Utrastruct Res 1971;34 :276-287 https://doi.org/10.1016/S0022-5320(71)80072-2
  9. Tamarin A: Submaxillary gland recovery from obstruction: II. Electron microscopic alterations in acirnar cells. J Ultrastruct Res 1971;34:288-302 https://doi.org/10.1016/S0022-5320(71)80073-4
  10. Harrison JD, Garrett JR: The effects of ductal ligation on the parenchyma of salivary glands of cat studied by enzyme histochemical methods. Histochem 1976;8:35-44 https://doi.org/10.1007/BF01004003
  11. Chung IK, Lee EW: An Experimental Study on Characteristics of $Na^{+}$, $K^{+}$-activated Adenosine Triphosphatase in Submaxillary Gland. J Kor Assoc Oral Maxillofac Surg 1983;1:152-162
  12. Emmelin N, Garrett JR, Ohlin P: Secretory activity and the myoepithelial cells of salivary glands after duct ligation in cats. Arch Oral Bilo 1974;19:275 https://doi.org/10.1016/0003-9969(74)90188-5
  13. Junqueira LCU: Cytological, cytochemical and biochemical observation on secreting and resting salivary gland. Exp Cell Res 1951;2:327 https://doi.org/10.1016/0014-4827(51)90022-5
  14. Lee JH, Kim JS: Physiology of saliva and salivary gland. Oral physiology 1989;175-207
  15. Epker BN: Obstructive and inflammatory diseases of the major salivary glands. Oral Surg 1972;33:2-27 https://doi.org/10.1016/0030-4220(72)90203-4
  16. Furstenberg AC: Diseases of the salivary glands. JAMA 1948;136:1 https://doi.org/10.1001/jama.1948.02890230001001
  17. Insacsson G: Salivary calculi and chronic sialadenitis of the submandibular gland : A radiographic and histologic study. Oral Surg 1984;58:622 https://doi.org/10.1016/0030-4220(84)90090-2
  18. Kruger GO: Textbook of oral and maxillofacial surgery, 6th Ed. 672, 1984
  19. Rankow RM, Polayers IM: Diseases of the salivary glands, W.B. Saunders Co., 1976
  20. Blatt IM: Studies in sialolithiasis, South Med J 1964;57:723 https://doi.org/10.1097/00007611-196406000-00022
  21. Beahrs OH, Woolner LB: Surgical treatment of disease of salivary glands. J Oral Surg 1969;27:119
  22. Bahn SL, Tabachnic TT: Sialolithisis of minor salivary gland. Oral Surg 1971;32:371 https://doi.org/10.1016/0030-4220(71)90196-4
  23. Roberg T: Sialolithisais. Ann Surg 1904;39:669-682 https://doi.org/10.1097/00000658-190405000-00005
  24. Tholen EF: Sialolithiasis. J Oral surg 1949;7:63-66
  25. New GB, Happer FR: Chronic inflammation of the salivary glands with or without calculi. Surg Gynecol Obestet 1931;53:456-460
  26. Kennett S: Bilateral submandibular sialolithiasis. Oral Surg Oral Pathol 1969;27:445 https://doi.org/10.1016/0030-4220(69)90069-3
  27. Lutcavage GJ, Schaberg SG: Bilateral submandibular sialolithisis and concurrent sialadenitis : A case report. J Oral & Maxillofac Surg 1991;49:1220 https://doi.org/10.1016/0278-2391(91)90421-H
  28. Kenefick JS: Some aspects of salivary gland disorders. Proc R Soc Med 1975;68:283
  29. Thoma KH: Cysts of the Salivary and mucous glands. Oral Pathology 1158, 1960
  30. Bailey H: Ranula. Brit DJ 1931;52:581
  31. Branner H: Pathology of ranula. Oral Surg 1949;20:1951
  32. Bhasker SN, Bloden TE, Weinmann JP: Pathogenesis of mucoceles. J Dent Res 1956;35:863-874 https://doi.org/10.1177/00220345560350060601
  33. Harrison JD, Garrett JR: Histological effects of ductal ligation of salivary glands of the cat. J Pathol 1976;118:245 https://doi.org/10.1002/path.1711180407
  34. Wallenborn WM, Sydnor TA, Hsu YT, Fitz-Huge GS: Experimental production of parotid gland atropy by ligation of stensen's duct and irradiation. Laryngoscope St. Louise, 1964;74:644
  35. Takai Y, Sumitomo S, Asano K, Mori M: Immunohistochemical observation of EGF and NGF in submandibular glands after duct ligation with or without testosterone administration. J Oral Pathol 1985;14:322-331 https://doi.org/10.1111/j.1600-0714.1985.tb00500.x
  36. Tandler B: Ultrastructure of chronically inflamed human salivary glands. Arch Pathol Lab Med 1977;101:425-431
  37. Standish SM, Shafer WG: Serial histologic effects of rat submaxillary and sublingual salivary gland duct and blood vessel ligation. J Dent Res 1957;36:866-879 https://doi.org/10.1177/00220345570360060801
  38. Bhasker SN, Cilly GE, Bhussry B: Regeneration of the salivary glands in the rabbit. J Dent Res 1966;45:37-41 https://doi.org/10.1177/00220345660450012601
  39. Shiba R, Hamada T, Kawakatsu K: Histochemical and electron microscopical studies on the effect of duct ligation of rat salivary glands. Arch Oral Biol 1972;17:299 https://doi.org/10.1016/0003-9969(72)90213-0