Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
권호 표지

2D AND 3D STRUCTURAL STUDY OF RETE RIDGE IN ORAL MUCOSA AND SKIN PADDLE OF VARIOUS FREE FLAPS

구강내 점막과 유리피판에 사용되는 피부의 rete ridge에 관한 2차원 및 3차원적 구조 연구

  • Ahn, Kang-Min (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Chung, Hun-Jong (Department of Pediatrics, Chung-Ju Medical Hospital, Kon-Kuk University) ;
  • Kim, Yoon-Tae (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Paeng, Jun-Young (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Shin, Young-Min (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Sung, Mi-Ae (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Park, Hee-Jung (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Myoung, Hoon (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Hwang, Soon-Jung (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Choi, Jin-Young (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Choung, Pill-Hoon (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Kim, Myung-Jin (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Lee, Jong-Ho (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University)
  • 안강민 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 정헌종 (건국대학교 의과대학 소아과) ;
  • 김윤태 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 팽준영 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 신영민 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 성미애 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 박희정 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 명훈 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 황순정 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 최진영 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 정필훈 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 김명진 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 이종호 (서울대학교 치과대학 구강악안면외과학교실)
  • Published : 2005.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objects : With the advancement of tissue engineering techniques, the effort to develop bioartificial mucosa have been actively delivered. The problem we met with this technique is the lack of mechanical strength between kerationocyte layer and dermal layer, where in the normal skin and mucosa, they are tightly bound with rete ridge structure. The purpose of this study is to understand the 2D and 3D structure of rete ridge of mucosa and skin paddle for rendering more biomimetic structure to the artificial mucosa. Materials and Methods : Oral mucosa and skin from the patients who received the oral surgery and maxillofacial reconstruction were harvested. The epidermis was separated from the dermis after treating with dispase for 12-16 hours. H&E staining was performed for 2D(dimensional) structure study and confocal LASER and SEM study were performed for 3D structure. Mean height(Sc) and arithmetic mean deviation(Sa) of all surface height were calculated. Results : The average height of rete ridge of skin flap was between $67.14{\mu}m$ and $194.55{\mu}m$. That of oral mucosa was between $146.26{\mu}m$ and $167.51{\mu}m$. Pressure bearing area and attached gingiva of oral mucosa showed deeper rete ridges. Conclusion : To obtain the adequate strength of artificially cultured keratinocyte skin and mucosa flap, it is necessary to imitate the original skin and mucosa structure, especially rete ridge. Through this study, 2D and 3D rete ridge structure of normal mucosa and skin was obtained. These results can be used as basis for substrate morphology for keratinocytes culture.

Keywords

References

  1. Nakamura T, Endo K, Cooper LJ, Fullwood NJ, Tanifuji N, Tsuzuki M, et al: The successful culture and autologous transplantation of rabbit oral mucosal epithelial cells on amniotic membrane. Invest Ophthalmol Vis Sci 2003;44(1):106-16 https://doi.org/10.1167/iovs.02-0195
  2. King RE, Altini M, Shear M: Basal cell extensions in human oral mucosa. J Oral Pathol 1979;8(3):140-6 https://doi.org/10.1111/j.1600-0714.1979.tb01820.x
  3. McMillan MD, Kerr MA: A light and scanning electron microscope study of epithelial thickenings and rete ridges in the adult hamster cheek pouch. Arch Oral Biol 1990;35(3):235-40 https://doi.org/10.1016/0003-9969(90)90061-E
  4. Moore RJ, McMillan MD, Gilbert RT: A scanning electron microscopic study of rete ridges in the cheek pouch of normal young, adult and aged Syrian hamsters. Arch Oral Biol 1992;37(5):337-41 https://doi.org/10.1016/0003-9969(92)90015-Z
  5. Klein-Szanto AJ, Schroeder HE: Architecture and density of the connective tissue papillae of the human oral mucosa. J Anat 1977; 123(1):93-109
  6. Pins GD, Toner M, Morgan JR: Microfabrication of an analog of the basal lamina: biocompatible membranes with complex topographies. Faseb J 2000;14(3):593-602 https://doi.org/10.1096/fasebj.14.3.593
  7. Vracko R: Basal lamina scaffold-anatomy and significance for maintenance of orderly tissue structure. Am J Pathol 1974;77(2):314-46
  8. Lavker RM, Sun TT: Epidermal stem cells. J Invest Dermatol 1983;81(1 Suppl):121s-7s https://doi.org/10.1111/1523-1747.ep12540880
  9. Rheinwald JG, Green H: Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 1975;6(3):331-43 https://doi.org/10.1016/S0092-8674(75)80001-8
  10. Kumagai N, Nishina H, Tanabe H, Hosaka T, Ishida H, Ogino Y: Clinical application of autologous cultured epithelia for the treatment of burn wounds and burn scars. Plast Reconstr Surg 1988;82(1):99- 110 https://doi.org/10.1097/00006534-198882010-00017
  11. Izumi K, Takacs G, Terashi H, Feinberg SE: Ex vivo development of a composite human oral mucosal equivalent. J Oral Maxillofac Surg 1999;57(5):571-7; discussion 77-8 https://doi.org/10.1016/S0278-2391(99)90077-0
  12. Ueda M, Ebata K, Kaneda T: In vitro fabrication of bioartificial mucosa for reconstruction of oral mucosa: basic research and clinical application. Ann Plast Surg 1991;27(6):540-9 https://doi.org/10.1097/00000637-199112000-00005
  13. Lauer G, Schimming R: Tissue-engineered mucosa graft for reconstruction of the intraoral lining after freeing of the tongue: a clinical and immunohistologic study. J Oral Maxillofac Surg 2001;59(2):169- 75; discussion 75-7 https://doi.org/10.1053/joms.2001.20489
  14. Lauer G, Schimming R, Frankenschmidt A: Intraoral wound closure with tissue-engineered mucosa: new perspectives for urethra reconstruction with buccal mucosa grafts. Plast Reconstr Surg 2001; 107(1):25-33 https://doi.org/10.1097/00006534-200101000-00005
  15. Yannas IV, Burke JF, Gordon PL, Huang C, Rubenstein RH: Design of an artificial skin. II. Control of chemical composition. J Biomed Mater Res 1980;14(2):107-32 https://doi.org/10.1002/jbm.820140203
  16. Bale E, White FH: Quantitative light and electron microscopical studies of the epithelial-connective tissue junction in intraoral mucosae. J Microsc 1982;128 (Pt 1):69-78 https://doi.org/10.1111/j.1365-2818.1982.tb00438.x
  17. Takami Y, Matsuda T, Yoshitake M, Hanumadass M, Walter RJ: Dispase/detergent treated dermal matrix as a dermal substitute. Burns 1996;22(3):182-90 https://doi.org/10.1016/0305-4179(95)00123-9
  18. Stenn KS, Link R, Moellmann G, Madri J, Kuklinska E: Dispase, a neutral protease from Bacillus polymyxa, is a powerful fibronectinase and type IV collagenase. J Invest Dermatol 1989;93(2):287-90 https://doi.org/10.1111/1523-1747.ep12277593