EXPRESSION OF E-CADHERIN WITH CORRELATION TO CLINICOPATHOLOGIC PARAMETERS IN ORAL SQUAMOUS CELL CARCINOMA

구강 편평세포암종에서 E-cadherin의 발현과 임상병리학적 지표와의 관계

  • Shin, Jae-Myoung (Department of Oral and Maxillofacial Surgery, The Catholic Univ. of Korea) ;
  • Kim, Young-Sill (Department of Pathology, College of Medicine, Cheju National University) ;
  • Kim, Chang-Hyen (Department of Oral and Maxillofacial Surgery, The Catholic Univ. of Korea) ;
  • Pyo, Sung-Woon (Department of Oral and Maxillofacial Surgery, The Catholic Univ. of Korea)
  • 신재명 (가톨릭대학교 의과대학 치과학교실 구강악안면외과) ;
  • 김영실 (제주대학교 의과대학 병리학교실) ;
  • 김창현 (가톨릭대학교 의과대학 치과학교실 구강악안면외과) ;
  • 표성운 (가톨릭대학교 의과대학 치과학교실 구강악안면외과)
  • Published : 2005.02.28

Abstract

It becomes more concerned that the cell adhesion molecule plays an important role in the process of malignant transformation and tumor behaviors including invasive growth and metastasis. It is postulated if the expression of adhesion molecule is reduced in tumor tissue, the tumor cell will be undifferentiated and lose their cell adhesion ability and polarity. So the tumor cells lost the adhesion of cell to cell and to basement membrane that they became more aggressive. Reduced cadherin expression enhances invasiveness through infiltrative growth and metastasis of tumor cells is well known and mostly accepted in many epithelia tumors. We explored the expression of E-cadherin by immunohistochemical staining in 50 oral squamous cell carcinomas and investigated the correlation between the expression of E-cadherin and clinicopathologic parameters and prognosis. The expression of E-cadherin was reduced in 40/50(80%) of primary tumors, and 21/22(95.5%) of lymph nodes. The reduced expression of the E-cadherin was associated with lymph node metastasis(P=0.029), invasive mode(P=0.030) and marginal status(P=0.038). Survival analysis showed that predictive period of E-cadherin reduced group(37 months) was lower than that of E-cadherin preserved group(60 months), but there was no statistical significant difference.

Keywords

References

  1. Pignatelli M, Vessey CJ: Adhesion molecule; Novel molecular tools in tumor pathology. Human Pathol 1994;25:849-856 https://doi.org/10.1016/0046-8177(94)90002-7
  2. Takeichi M: Cadherin; Cell adhesion receptor as a morphogenetic regulator. Science 1991;251:1451-1455 https://doi.org/10.1126/science.2006419
  3. Takeichi M: The Cadherin; Cell-cell adhesion molecules controlling animal morphogenesis. Development 1988;102:639-655
  4. Frixen UH, Behrens J, Sachs M, Eberle G, Voss B, Wawrda A, et al: E-cadherin-mediated cell-cell adhesion prevent invasiveness of human carcinoma cells. J Cell Biol 1991;173:166-1691
  5. Nagafuchi A, Taketi M, Tsukita S: The 102kD cadherin-associated protein; Similarity to vinculin and post-transcriptional regulation of expression. Cell 1991;65:849-857 https://doi.org/10.1016/0092-8674(91)90392-C
  6. Shiozaki H, Tahara H, Oka H, Miyata M, Kobayashi K, Tamura S, et al: Expression of immunoreactive E-cadherin adhesion molecules in human cancers. Am J Pathol 1991;139:17-23
  7. Oka H, Shiozaki H, Tahara H, Tamura S, Miyata M, et al: Immunohistochemical evaluation of E-cadherin adhesion molecule expression in human gastric cancer. Virchows Arch Abt A Pathol Anat 1992;421:149-156 https://doi.org/10.1007/BF01607048
  8. Schipper JH, Unger A, Jahnke K: E-cadherin as a functional marker of the differentiation and invasiveness of squamous cell carcinoma of the head and neck. Clinical Otolaryn Allied Sci 1994;19:381-384 https://doi.org/10.1111/j.1365-2273.1994.tb01252.x
  9. Sander DS, Bruton R, Darnton SJ, Casson AG, Hanson I, Williams HK, Jankowski J: Sequential change in cadherin-catenin expression associated with the progression and heterogenicity of primary oesophageal squamous cell carcinoma. Int J Cancer 1998;79:573-579 https://doi.org/10.1002/(SICI)1097-0215(19981218)79:6<573::AID-IJC4>3.0.CO;2-H
  10. Zheng Z, Pan J, Chu MB, Wong YC, Cheung AL, Taso SW: Downregulation and abnormal expression of E-cadherin and $\beta$- catenin in nasopharyngeal carcinoma; Close association with advanced disease stage and lymph node metastasis. Human Pathol 1999;30:458-466 https://doi.org/10.1016/S0046-8177(99)90123-5
  11. William HK, Sanders DS, Jankowski PA, Landini G, Brown AM: Expression of cadherins and catenins in oral epithelial dysplasia and squamous cell carcinoma. 1998;27:308-317
  12. Spiro RH, Alfonso AE, Farr FW: Cervical lymph node metastasis from epidermoid carcinoma of oral cavity and oropharynx. Am J Surg 1974;128:562-567 https://doi.org/10.1016/0002-9610(74)90276-1
  13. 이상화, 표성운, 김영실, 최목균: 설편평세포암에서 CD44, MMP- 2, MMP-9, TIMP-1의 발현. 대악성외지 2003;25:273-282
  14. 윤경인, 표성운, 김영실, 이철원: 혀의 편평상피세포암에서 MMP-2, MMP-3, MMP-10, MMP-13의 발현양상. 대구악외지 2002;28:182-187
  15. Gumbiner BM: Cell adhesion; The molecular basis of tissue architecture and morphogenesis. Cell 1996;84:345-357 https://doi.org/10.1016/S0092-8674(00)81279-9
  16. Shimoyama Y, Nagafuchi A, Fujita S, Gotoh M, Takeichi, Tsukita S, Hirohashi S: Cadherin dysfunction in a human cancer line; Possible involvement of loss of $\alpha$-catenin expression in reduced cell-cell adhesiveness. Cancer Res 1992;52:5770-5774
  17. Oyama T, Kanai Y, Ochiai A, Akimoto S, Oda T, Yanagihara K, et al: A truncated $\beta$-catenin disrupts the interaction between E-cadherin and $\alpha$-catenin; A cause of loss of intercellular adhesiveness in human cancer cell lines. Cancer Res 1994;54:6282-6287
  18. Nakanishi Y, Ochiai A, Akimoto S, Kato H, Watanabe H, Tachimori Y, et al: Expression of E-cadherin, $\alpha$-catenin, $\beta$-catenin and plakoglobin in esophageal carcinoma and its prognostic significance. Oncology 1997;54:158-165 https://doi.org/10.1159/000227681
  19. Kimura Y, Shiozaki H, Doki Y, Yamamoto M, Utsinomiya T, Kawanishi K, et al: Cytoplasmic $\beta$-catenin in esophageal cancers. Int J Cancer 1999;84:174-178 https://doi.org/10.1002/(SICI)1097-0215(19990420)84:2<174::AID-IJC14>3.0.CO;2-E
  20. Bagutti C, Speight PM, Watt FM: Comparison of integrin, cadherin and catenin expression in squamous cell carcinoma of the oral cavity. J Pathol 1988;186:8-16 https://doi.org/10.1002/(SICI)1096-9896(199809)186:1<8::AID-PATH156>3.0.CO;2-H
  21. 이수진: 설 편평세포암종에서 E-Cadherin과 $\beta$-Catenin의 발현. 가톨릭대학교 대학원논문집, 서울, 2003
  22. Horn KV, Tang DG: Adhesion molecules and tumor cell interaction with endothelium and subendothelial matrix. Cancer Metastasis Rev 1992;11:353-357 https://doi.org/10.1007/BF01307187
  23. Tanaka N, Sugiahara K, Odajima T, Mimura M, Kimijima Y, Ichinose S: Oral squamous cell carcinoma -Electron microscopic and immunohistochemical characteristics. Med Electron Microsc 2002; 35:127
  24. 박순철: 위암 환자에서 E-cadherin 및 CD44v6 발현에 따른 재발 위험도. 가톨릭대학교 대학원논문집, 서울, 2003