Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
권호 표지
DOI QR코드

DOI QR Code

In vitro antioxidant activity of various extracts of Aristolochia bracteolata leaves

  • Published : 2005.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Aristolochia bracteolata is being used in traditional medicine for a variety of ailments. Three successive and two crude extracts of its leaves were subjected for in vitro antioxidant activity using seven different methods. The successive ethyl acetate and crude methanol extracts have shown potent antioxidant activity in ABTS method with $IC_{50}$ values of $17.08\;{\pm}\;0.44\;and\;28.12\;{\pm}\;2.87\;{\mu}g/ml$, respectively. The crude methanol and successive chloroform extracts have shown moderate antioxidant activity in scavenging of hydroxyl radical by p-NDA method. All the extracts have shown moderate to low antioxidant activity by other methods.

Keywords

References

  1. Anonymous. The Wealth of India: Raw Materials, (1985) Vol l(A), pp. 422-423, Council of Scientific and Industrial Research, New Delhi.
  2. Badami S, Mahesh KG, Suresh B. (2003) Antioxidant activity of the ethanolic extract of Striga orobanchioides. J. Ethnopharmacol. 85, 227-230. https://doi.org/10.1016/S0378-8741(03)00021-7
  3. Barry H, John MCG, Okeizie IA. (1987) The Deoxyribose method: A simple test-tube assay for determination of rate constant for reactions of hydroxyl radicals. Anal. Biochem. 165, 215-219. https://doi.org/10.1016/0003-2697(87)90222-3
  4. Elizabeth K, Rao MNA. (1999) Oxygen radical scavenging activity of curcumin. Int. J. Pharm. 58, 237-240. https://doi.org/10.1016/0378-5173(90)90201-E
  5. Garg SC. (2000) Ethnomedicine for snake bite. J. Med. Aroma. Plant Sci. 22, 23, 546-553.
  6. Garrat DC. (1964) The Quantitative Analysis of Drugs. 3rd Ed. pp. 456-458, Chapman and Hall Ltd., Japan.
  7. Guddadarangavvanahally KJ, Lingamallu JR, Kunnumpurathu KS. (2004) Antioxidant activities of flavidin in different in vitro model systems. Bioorg. Med. Chem. 12, 5141-5147. https://doi.org/10.1016/j.bmc.2004.07.028
  8. Halliwell B. (1994) Free radicals and antioxidants: A personal view. Nutrition Reνiews 52, 253-265.
  9. Hwang BY, Kim HS, Lee JH, Hong YS, Lee KS, Lee JJ. (2001) Antioxidant activity of benzoylated flavon-3-ol glycosides from Celastrus 0rbiculatus. J. Nat. Prod. 64, 82-84. https://doi.org/10.1021/np000251l
  10. Marcocci L, Packer L, Sckaki A, Albert GM. (1994) Antioxidant action of Ginkgo biloba extract EGb 766. Methods Enzymol. 234, 462-475. https://doi.org/10.1016/0076-6879(94)34117-6
  11. Jaggi RK, Kapoor S. (1999) Flavonoids - a review of biological activities. Indian Drugs. 36, 668-676.
  12. Re R, Pellegrini M, Proteggente A, Pannala A, Yang M, Rice Ee. (1999) Antioxidant activity applying an improved ABTS radical cation decolorisation assay. Free Radical Biol. Med. 26, 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  13. Satyavati GV, Raina MK, Sharma M. (1976) Medicinal Plants of India Vol 1, pp. 287, Indian Council of Medical Research, New Delhi.
  14. Shirwaikar A, Somashekar AP. (2003) Antiinflammatory activity and free radical scavenging studies of Aristolochia bracteolata. Indian J. Pharm. Sci 65, 67-69.
  15. Shirwaikar A, Somashekar AP, Udupa AL, Udupa SL, Somashekar S. (2003) Wound healing studies of Aristolochia bracteolata Lam. with supportive action of antioxidant enzymes. Phytomedicine 10, 558-562. https://doi.org/10.1078/094471103322331548
  16. Shreejayan M, Rao MNA. (1996) Free radical scavenging activity of curcuminoids. Arzneim-Forsch. Drug Res. 46, 169-171.
  17. Tripathi YB, Chaurasia S, Tripathi E, Upadhyay A, Dubey GP. (1996) Bacopa monniera Linn. as an antioxidant. Mechanism of action. Indian J. Exp. BioI. 34, 523-526.
  18. Velazquez E, Tournier HA, Mordujovich BP, Saavedra GF, Schinella GR. (2003) Antioxidant activity of Paraguayan plant extracts. Fitoterapia 74, 91-97. https://doi.org/10.1016/S0367-326X(02)00293-9