Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Brine shrimp lethality and cytotoxicity assay of Araucaria bidwillii Hook in human carcinoma cell lines

  • Ahamed, KFH Nazeer (School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Kumar, V (School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Manikandan, L (School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Wahile, Atul M (School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Mukherjee, Kakali (School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Saha, BP (School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Mukherjee, Pulok K (School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University)
  • Published : 2005.03.30
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Abstract

The leaf extracts of Araucaria bidwillii Hook. (Araucariaceae) were evaluated for their cytotoxic effect in various human cancer cell lines. Preliminary investigation by brine shrimp lethality assay indicated that $LC_{50}$ value of various successive extracts were found to be less than $1000\;{\mu}g/ml$, where the ethyl acetate extract showed maximum activity of less than $100\;{\mu}g/ml$. Further cytotoxic evaluation of various leaf extracts of Araucaria bidwilli Hook was carried out in four different human cancer cell lines-acute myeloblastic leukemia (HL-60), chronic myelogenic leukemia (K-562), breast adenocarcinoma (MCF-7) and cervical epithelial carcinoma (HeLa). Cytotoxicity was assessed by trypan blue dye exclusion method and 3-(4,5-dimethyl thiazole-2yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay. From the present investigation it was found that the ethyl acetate and methanol extract of Araucaria bidwilli Hook was found to be more effective in leukemic cell lines and was less effective in MCF-7 and HeLa. The $IC_{50}$ value of the ethyl acetate extract in leukemic cell lines was found to be $28.18\;and\;34.64\;{\mu}g/ml$ and methanol extract was found to be $33.11\;&\;39.81\;{\mu}g/ml$. It can be concluded that various extract from the leaves of Araucaria bidwillii Hook. posses cytotoxic activity tested in brine shrimps and various human carcinoma cell lines.

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References

  1. Anderson EF. (1972) Ethnobotany of hill tribes of northern Thailand II, Lahu Medicinal Plants. Econ. Bot. 4, 442-450.
  2. Dahanukar SR, Kulkarni A, RegeNN. (2000) Phannacology of medicinal plants and natural products. Indian J. Pharmacol. 32, S81-S118.
  3. De Rosa S, De giulio A, Iodice C. (1994) Biological effects of phenylated hydroquinone; structure activity relationship studies in antimicrobial, brine shrimp, and fish lethality assays. J. Nat. Prod. 7, 1711-1716.
  4. Dhanasekaran S, Ravisankar S, Sumitra devi SK, Suresh B,Sethuraman M, Rajan S. (1993) Pharmacological studies of Araucaria bidwillii Hook. Ancient Science of Life 13, 137-142.
  5. Dhanasekaran S, Ravisankar S, Sumitra devi SK, Suresh B, Sethuraman M, Rajan S. (1994) Pharmacological studies of Araucaria bidwilliiHook. Geobios New Reports 13,49-52.
  6. Fransworth NR, Soejarto DO. (1985) Potential consequences of plant extinction in the United States on the current and future availability of prescription drugs. Econ. Bot. 39,231-240. https://doi.org/10.1007/BF02858792
  7. Francis D, Rita L. (1986) Rapid calorimetric assay for growth and survival: modification to tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Met. 89, 271-277. https://doi.org/10.1016/0022-1759(86)90368-6
  8. Fikrat IL. (2002) Omcer chemopreventive and tumoricidal properties of saffron (Crocus sativus L.). Exp. Bioi. Med. 227, 20-25. https://doi.org/10.1177/153537020222700104
  9. Finney D. (1971) Probit Analysis, 3rd ed, pp 76-80. Cambridge University Press, Cambridge, UK.
  10. Grosch DS. (1967) Poisoning with DDT: Effect on reproductive performance of Artemia. Science 155, 592-593. https://doi.org/10.1126/science.155.3762.592
  11. Granade HR, Cheng PC, DoorenbosNJ. (1976) Gguatera I: brine shrimp (Artemia salina L) larval assay for ciguatera toxins. J. Pharm. Sci. 65, 1414-1415. https://doi.org/10.1002/jps.2600650943
  12. Geran RI, Greenberg NH, Macdonald NN, Schumacher AA, Abbott BJ. (1972) Cancer Chemother. Rep. 3,1-103.
  13. Suffness M, Douros JD. (1982) Current Status of NCI Plant and Animal Product Program, J. Nat. Prod. 45, 1-14. https://doi.org/10.1021/np50019a001
  14. Guha SRD, Singh MM, Singh SV, Kumar A, Bist DPS. (1971) Kraft pulping of Cupressus lusiianica and Araucaria bidwillii. Indian Forestry 97, 542-546.
  15. Hora B. (1981) The Oxford Encyclopedia of Tress of the World, p. 60, Oxford University Press, Oxford.
  16. Hamill RL, Higgens CE, Boaz HE. (1969)The structure of beauvericin, a new depsipeptide antibiotic toxic to Artemia salina. Tetrahed. Lett. 49, 4255-4258.
  17. Khan NU, Ilyas M, Rahman W, Ikigawa M, Kawano N. (1970) Bisflavones in the leaves of Araucaria bidwilli Hooker. Tetrahed. Lett. 33,2941-2944.
  18. Khan NU, Ansari WH, Usmani JN, Ilyas M, Rahman W. (1971) Biflavones of Araucariales. Phytochemistry 10, 2129-2131. https://doi.org/10.1016/S0031-9422(00)97208-X
  19. Khan NU, Ilyas M, Rahman W, Mashima T, Okigawa M, Kawano N. (1972) Biflavones from the leaves of Araucaria bidwilli Hooker and Agathis alba foxworthy (Araucariaceae) Tetrahedron 28, 5698-5695.
  20. Ilyas N, Ilyas M., Rahman W, Okigawa M, Kawano N. (1978) Biflavones from the leaves of Araucaria excelsa. Photochem. 17, 987-990. https://doi.org/10.1016/S0031-9422(00)88662-8
  21. Meyer BN, Ferrigni NR, Putnam JE, Jacobson LB, Nichols DE, McLaughlin JL. (1982)Brine Shrimp: A convenient general bioassayfor active plant constituent Planta Med. 45, 31-34. https://doi.org/10.1055/s-2007-971236
  22. McLaughlin JR. (1991) Crown gall tumors on potato discsand brineshrimp leathlity:Two simple bioassays for higher plant screening and fractionation. In: Methods in plantbiochemistry, edited by Hostettman K, Dey PM, Harborne, p.2-30, Academic press, NY.
  23. Mukherjee PK. (2001)Evaluation of Indian traditional medicine, DrugInfonnation J. 35, 623-632.
  24. Mukherjee PK. (2002) Quality Control ofHerbal DrugsAn Approach to Evaluation of Botanicals, pp. 90-98, Business Horizons, New Delhi, India.
  25. Mukherjee PK. (2003) Exploring botanicals in Indian systems of medicine-regulatory perspectives, Clinical Research and Regulatory Affairs 20, 249-264. https://doi.org/10.1081/CRP-120023840
  26. Mukerjee PK. (2003a) GMP for production of phytomedicine. In: GMFfor Botanicals - Regulatory andquality issues onPhytomedicines, edited by Mukerjee PK, Verpoorte R, p. 99-112, Business Horizons, New Delhi, India.
  27. Prashanth OS, Deepak M, Rani P, Kadamboor S, Mathew A, Chandrashekar AP and Agarwal A. (2002). Brine shrimp Lethality assay of Bacopa monnieri. Phytother. Res. 16, 197-198. https://doi.org/10.1002/ptr.1001
  28. Rajan S, Sethuraman M, Mukherjee PK. (2002) Ethnobiology of the nilgiris hills, India. Phytother. Res. 16, 98-116. https://doi.org/10.1002/ptr.1098
  29. Sam TW. (1993) Toxicity testing using the brine shrimp: Artemia salina. In: Bioactive Natural Products, edited by Colegate SM, Molyneux RJ, pp. 441-454, CRC press Inc., Boca Raton.
  30. Tyler VE, Lynn R.B, James ER. (1988) Phannacognosy, 9th ed, Philadelphia, Lea & Febiger.