Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation, Identification and Biological Control Activity of SKU-78 Strain against Ralstonia solanacearum

풋마름병균, Ralstonia solanacearum의 길항세균 SKU-78 균주의 분리 동정 및 특성

  • Sung, Pil-Je (Department of Biological Engineering, Seokyeong University) ;
  • Shin, Jeong-Kun (Korea Biochemical Co.) ;
  • Cho, Hong-Bum (Department of Biological Engineering, Seokyeong University) ;
  • Kim, Shin-Duk (Department of Biological Engineering, Seokyeong University)
  • Published : 2005.03.31
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Abstract

Six stains of plant growth promoting rhizobacteria were selected through germinating seed assay and root colonization assay. Among them, SKU-78 strain induced significant suppression of bacterial wilt disease in tomato and pepper plants. Seed treatment followed by soil drench application with this strain resulted in over 60% reduction of bacterial wilt disease compared with the control. It was suggested that SKU-78 strain activated the host defense systems in plants, based on lack of direct antibiosis against pathogen. According to Bergey's Manual of Systemic Bacteriology and 16S rDNA sequence data, SKU-78 stain was identified as Bacillus sp. SKU-78.

종자발아실험과 뿌리에서의 집락 형성실험으로 고추와 토마토의 근권 토양에서 plant growth promoting rhizobacteria(PGPR) 균주를 선발하였다. 그 중 포트실험에서 종자처리와 토양 관주처리 하였을 때 60% 이상 풋마름병 방제효과를 나타낸 SKU-78 균주에 대해서 포장에서의 방제효과 검증에 의해 생물농약으로의 개발 가능성을 확인하였으며, 생화학적 특성조사와 16S rDNA sequence 분석에 의해 SKU-78 균주를 Bacillus sp. SKU-78로 동정하였다.

Keywords

References

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