한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제13권4호
  • /
  • Pages.194-198
  • /
  • 2005
  • /
  • 1225-9306(pISSN)
  • /
  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

가시오갈피 유식물체 추출물의 항산화 활성물질

Antioxidative Compounds in Extracts of Eleutherococcus senticosus Max. Plantlets

  • Kim, Myong-Jo (Division of Bio-resources Technology, Kangwon National University) ;
  • Kwon, Yong-Soo (College of Pharmacy, Kangwon National University) ;
  • Yu, Chang-Yeon (Division of Bio-resources Technology, Kangwon National University)
  • 발행 : 2005.08.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

가시오갈피 유식물체의 함유성분을 밝히고, 분리된 화합물들을 대상으로 DPPH를 이용한 항산화활성을 측정하여 대체 약용자원으로서의 사용 가능성을 검토하기 위하여 가시오갈피 유식물체를 MeOH로 추출, 농축하고 Hexane, EtOAc 및 BuOH순으로 분획하여 얻은 각각의 분획을 대상으로 성분연구에 착수하였다. 각종 column chromatography를 이용하여 성분을 분리한 결과 EtOAc분획으로부터 1종의 화합물을 분리하였고, BuOH 분획으로부터 1종의 화합물을 분리하였다. 이들 화합물에 대하여 UV, IR, Mass, $^1H-NMR$ 및 $^{13}C-NMR$ spectrum과 문헌치를 대조하여 그 구조를 동정한 결과그 구조는 각각 Chlorogenic acld (1)과 1,4-di-O-caffeoyl-quinic acid (2)이였다. DPPH (1,1-diphenyl-2-picry-hydrazyl) free radical scavenging activity를 이용하여 측정한 항산화활성의 $RC_{50}$ value는 각각 1.2 및 $0.4\;{\mu}g/ml$였다. 이상의 결과로부터 가시오갈피 유식물체 (Eleutherococcus senticosus Max.)는 천연 항산화제로서의 개발가능성이 높다고 사료된다.

Two antioxidative compounds were isolated from the methanolic extract of Eleutherococcus senticosus Max. plantlets and identified as chlorogenic acid and 1,4-di-o-caffeoyl-quinic acid on the basis of mass spectroscopy, $^1H-NMR$ and $^{13}C-NMR$ data. The DPPH free radical scavenging activities of chlorogenic acid $(RC_{50}\;:\;1.2\;{\mu}g)$ and 1,4-di-o-caffeoyl-quinic acid $(RC_{50}\;:\;0.4\;{\mu}g)$ were more effective than those of ${\alpha}-tocopherol\;(RC_{50}\;:\;12\;{\mu}g)$. Of them, 1,4-di-o-caffeoyl-quinic acid compound were isolated for the first time from this plant.

키워드

참고문헌

  1. Fitoterapia v.71 Major dicaffeoylquinic acids from Artemisia vulgaris Andree, C.;Annie, H.;Didier, F.;Andre, P.C.;Jean, L.L. https://doi.org/10.1016/S0367-326X(00)00163-5
  2. Izv Sibir Otdel Akad Nauk USSR v.9 A new medicinal plant of the family Araliceae the spiny Eleutherococcus Brekhman, I.I.
  3. Fitoterapia v.73 A new dicaffeoylquinic acid butyl ester from lsertia pittieri Byung, H.U.;Merve, P.;Annelise, L.;Bernard, W.;Raul, A.;Lieve, D.;Robert, A. https://doi.org/10.1016/S0367-326X(02)00181-8
  4. Kor. J. Pharmacology v.24 Screening for antioxidant activity of plants and marine algae and its active principles from Prunus daviana Choi, J.S.;Park, J.H.;Kim, H.G.;Young, H.S.;Mun, S.I.
  5. Life Sciences A phenolic compound, 5-caffeoylquinic acid(chlorogenic acid), is a new type and strong matrix metalloproteinase-9 inhibitor: Isolation and identification from methanol xetract of Euonymus alatus Jin, U.H.;Kang, S.K.;Kim, J.K.;Park, W.H.;Kim, J.G.;Moon, S.K.;Kim, C.H.
  6. Kor. J of Pharmocol v.10 Distribution of Acanthopanax plants in Korea Lee, W.T.
  7. Korean J. Medicinal Crop Sci. v.12 no.6 Long-trem cold storage and plant regeneation of suspension cultured somatic embryos of Eleutherococcus senticosus Maxim Li, C.H.;Lim, D.L.;Heo, K.;Kim, K.J.;Lee, C.O.;Lee, J.G.;Cui, X.H.;Yu, C.Y.
  8. Life Sciences v.71 Effects of caffeoyl conjugates of isoprenylhydroquinone glucoside and quinic acid on leukocyte function Luis, G.;Rosa, M.G.;Salbador, M.;Mariadel, C.R.;Guillermo, S.;Jose, L.R. https://doi.org/10.1016/S0024-3205(02)02167-7
  9. European journal of Pharmaceutical Sciences Oxidative metabolism of 5-o-caffeoylquinic acid(chlorogenic acid), a bioactive natural product, by metalloporphyrin and rat liver mitochondria Michel, D.S.;Patricia, R.M.;Pierre, A.S.;Renato, B.;Iamamoto, Y.;Norberto, P.L.
  10. Anal. Biochem. v.95 Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction Ohkawa, H.;Ohishi, N.;Ygi, K. https://doi.org/10.1016/0003-2697(79)90738-3
  11. Phytochemistry v.24 no.2 Application of fast atom bombardment mass spectrometry to chlorogenic acids Sakushima, A.;Hisada, S.;Nishibe, S.;Brandenberger, H. https://doi.org/10.1016/S0031-9422(00)83545-1
  12. Phytochemistry v.27 no.2 Phenylpropanoid glucose esters from Prunus buergeriana Shimomura, H.;Sashida, Y.;Adachi, T. https://doi.org/10.1016/0031-9422(88)83165-0
  13. Natural Product Sciences v.5 no.2 Flavonoids and chlorogenic acid from Eriobotrya japonica scavenge peroxynitrite Soung, D.Y.;Kim, J.S.;Chung, H.Y.;Jung, H.A.;Park, J.C.;Choi, J.S.
  14. Chinese drugs of plant origin Tang, W.;Eisenbrand, G.