Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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In vitro Growth of Shoot Derived from Shoot Tip in Asparagus cochinchinensis (Lour.) Merr.

천문동 경정 유래 신초의 기내생장

  • Published : 2005.08.30
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Abstract

This study was carried out to investigate the optimal conditions for efficient in vitro growth of Asparagus cochinchinensis (Lour.) Merr. shoot derived from shoot tip. Shoots were successfully cultured in MS medium. It was found that $23^{\circ}C{\sim}25^{\circ}C$ were suitable for shoot growth. The growth of shoot was greatly influenced by cytokinins. The shoots derived from shoot tip were well elongated on MS medium supplemented with BA and zeatin. In vitro shoots were very poor or no growing. Especially, 3.0 mg/l BA on MS medium was very effective in elongation of shoot. Root formation from in vitro growth of plant let was achieved on MS medium supplemented with 0.5 mg/l IBA. The results suggest that selection of plant growth regulator could be important factor to achieve an efficient in vitro growth.

천문동 (Asparagus cochinchinesis (Lour.) Merr.)의 기내생장과 증식의 가능성을 검토하기 위하여 신초의 유기, 생장 및 발근에 적합한 배지, 식물생장조절제, 배양온도를 검정한 결과를 요약하면 다음과 같다. MS 기본배지에 3.0 mg/l BA 처리시 신초의 유기 및 생장이 가장 양호하였으며 MS 기본배지에 0.5 mg/l IBA 처리가 발근에 가장 효과적이었고 기내배양 온도는 $23{\sim}25^{\circ}C$가 적합하였다.

Keywords

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