Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Chemical Standardization of Poria cocos

복령의 품질표준화를 위한 지표성분 탐색 및 정량법 개발

  • Hoang, Lam (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kwon, Soon-Ho (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kim, Kyung-Ah (Department of Natural Fiber, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Hur, Jong-Moon (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kang, Young-Hwa (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Song, Kyung-Sik (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University)
  • 황람 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 권순호 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 김경아 (경북대학교 농업생명과학대학 천연섬유학과) ;
  • 허종문 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 강영화 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 송경식 (경북대학교 농업생명과학대학 응용생명과학부)
  • Published : 2005.09.30
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Abstract

For the purpose of the quality control of Poria cocos, three major compounds were isolated and identified as pachymic acid (1), $3{\beta}-hydroxylanosta-7,9(11),24-trien-21-oic$ acid (2), and dehydroeburicoic acid (3). The optimal extraction conditions for the quantification of pachymic acid its analogues were the 3 hours of reflux with 15g of P. cocos in 100ml 95% ethanol. HPLC conditions were as follows: Column; ZORBAX Eclipse XDB C18 $(4.6{\times}250\;mm,\;Agillent)$, mobile phase; 1% HOAc in 70% $MeOH{\rightarrow}1%$ HOAc in 100% MeOH for 25 min, then 1% HOAc in 100% MeOH for 15 min, detector, ELSD, flow rate; 1ml/min. The mean contents of 1,2, and 3 in Poria cocos cultivated at 18 different site were $0.65{\pm}0.19,\;0.88{\pm}0.72,\;and\;0.84{\pm}0.54\;mg/g$, respectively, and values might be the guide line for the quality control of P. cocos.

Keywords

References

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