Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Flavonoids of Gomphocarpus sinaicus and Evaluation of Some Pharmacological Activities

  • Published : 2005.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

The aerial parts of Gomphocarpus sinaicus Boiss. yielded four flavonoids that were identified as isorhamnetin 3-O-rhamnoglucoside (1), luteolin-7-O-glucoside-3-O-rhamnoside (2), rutin (3) and rutin-7-O-rhamnoside (4). All of the isolated flavonoids were identified by spectroscopic methods (UV, FAB-MS, $^1H-NMR\;&\;^{13}C-NMR$) and in comparison with the literature data. The isolated flavonoids 1, 2 and 4 are reported here for the first time from Gomphocarpus sinaicus Boiss. Three sets of experiments were carried out using the defatted alcoholic extract of Gomphocarpus sinaicus Boiss: the $1^{st}$ experiment indicated that the $LD_{50}$ was 49.82 mg/100 g b.wt. of intraperitoneally (i.p.) injected mice. The toxic signs were recorded within the first 24 hr post-injection. The $2^{nd}$ experiment revealed that the extract of the plant exhibited significant anti-inflammatory effects in normal rats. The $3^{rd}$ experiment was found that the tested doses of the extract in diabetic rats induced a significant decrease in serum glucose, AST, ALT, triglycerides, cholesterol and LDL, while HDL caused a significant increase.

Keywords

References

  1. Abdel-Azim, N.S., Phytochemistry 49, 273-275 (1998) https://doi.org/10.1016/S0031-9422(97)00848-0
  2. Abdel. Azim, N.S., Hammouda, F.M., Hunkler, D., and Rimpler, H., Phytochemistry 42, 523-529 (1996) https://doi.org/10.1016/0031-9422(95)00940-X
  3. Armitage, P. Statistical Methods in Medical Research, Blackwell Scientific Publications, London (1971)
  4. Chitmel, H.R., Chandra, R., and Kaushik, S., Studies on antidiarrheal activity of calotropis gig antea R. BR. In experimental animals. Pharm Pharmaceut Sci. 7, 70-75 (2004)
  5. Ferrel, J.E., Chang-sing, P.D., Loew, G., King, R., Mansour, J.M, and Mansour, T.E., Structure/activity studies of flavonoids as inhibitor of cAMPphosphodiestase and relationship to quantum chemical indices. Mol Pharmacol 16, 556 (1979)
  6. Fossatip, P., Enzymatic determination of seum triglycerides. Principle Clin. Chem. 28, 2077 (1982)
  7. Furst, P., Antioxidative power of non-nutritive substances in food stuffs. Ernahrung 19, 457-460 (1995)
  8. Gupta, J. and Ali Mohd., Indian J. Pharm Sci. 62, 29-32 (2000)
  9. Harborne, J.B. and Day P.H. 'Methods in Plant Biochemistry' Volume 1, Plant Phenolics, Academic Press, London (1982)
  10. Harborne, J.B. and Marbry, T.J. 'The Flavonoids : Advances in Research' Chapman and Hall Ltd. Printed in Great Britain at the University Press, Cambridge (1982)
  11. Heinrich, M., Robles, M., West, J.E., Ortiz-de-Monlellano, B.R. and Rodriguez, E. Annu Rev. Pharmacol Toxicol. 38, 539-65 (1998) https://doi.org/10.1146/annurev.pharmtox.38.1.539
  12. Kapure , J.N. and Saxena, H.C., Mathematical Statistics, S. Chand and Co. Ltd. , Ram. Nagar, New Delhi (1972)
  13. Karber, G, Bietarg Zur Kollectiven behandlung Pharmakoligischem reinhen versuche. Arch Exp. Pathol. Pharamakol. 162, 480-482 (1931) https://doi.org/10.1007/BF01863914
  14. Khushbaktova, Z.A., Syrov, V.N., and Batirov, E. Kh., Effect of flavonoids on the course of hyperlipidemia and atherosclerosis under experimental condition. Khimiko Farmatsevticheskii Zhurnal. 25, 53-57. (1991)
  15. Koike, K., Bevelle, C.S., Talapatra, K., Cordell, G.A. and Farnsworth, N.R., Chem. Potenial anticancer agents. V. cardiac glycosides of asclepias albicans (Asclepiadaceae). Pharm. Bull. 28, 401 (1980)
  16. Korthuis R.J., Benoit J.N., and Kvietys P.R., Intestinal hypermia in experimental diabetes mellitus. Am. J. Physiol. 253, G 26-G 32. (1987)
  17. Marbry, T.J., Markham, K.R., and Thomas, M.B. 'The Systematic identification of Flavonoids', Springer Verlag. Berlin-Heideberg New York (1970)
  18. Markham, K.R., 'Techniques of Flavonoid Identification' Academic Press, London (1982)
  19. Middleton, E., Effect of plant flavonoids on immune and inflammatory cell function. In flavonoids in the living system, ed. Manthey and Buslig. Plenum Press, NY 175-182 (1998)
  20. Murray, R.K., Granner, D.K., Mayes, P.A., and Rodwell, V.W., Harper's Biochemistry, 23rd ed., Appleton Lange, Norwalk, San Mateo, California (1993)
  21. Rahman, M.A. and Wilcock, C.C., Bangladesh J. Bot. 20, 175-178 (1991)
  22. Reitman, S. and Frankel, S., Colorimetric method for aspirate and alanine tranferases. Am. J. Clin. Pathol. 28, 56-63 (1957)
  23. Richmond, W., Preparation and properties of a cholesterol oxidase from norcardia sp and its application to the enzymatic assay of total cholesterol in seum. Clin. Chem. 19, 1350 (1973)
  24. Sarg, T., El-Domiaty, M., Abdel Aziz, E., and Abou-Hsharn, M., Egypt. J. Pharm. Sci. 34, 77-585 (1993)
  25. Sen, S. and Sahu, N.P., Phytochem 31, 2919-21 (1992) https://doi.org/10.1016/0031-9422(92)83668-O
  26. Shibuya, H. and Zhang, R.S., Chem. Pharm. Bull. Tokyo 40, 2647-53 (1992) https://doi.org/10.1248/cpb.40.2647
  27. Tackholm, V., Students Flora of Egypt, second edition, pp. 433 (1974)
  28. Trinder, P., Determination of glucose in blood using glucose oxidase with alternative oxygen acceptor. Ann. Clin. Biochem. 6, 24 (1969)
  29. Winter C.A., Risley, E.A. and Huss G. W., Carragenan-induced edema in hind paw of the rat as an assay for anti-inflammatory drugs. Proc. Soc. Exp. Biol. Med. 111, 544-52 (1962)
  30. Zhang, Q., ZHO, Y., Wang, B., and Guangzhong Tu., New triterpenoid saponins from stelmatocryptonkhasianum. Chem. Pharm. Bull. 5, 574-578 (2003)