The Effects of Chungpyesagan-tang on the Recovery of Liver Function in Rat Injured by $CCl_4$

청폐사간탕(淸肺瀉肝湯)이 $CCl_4$로 유발(誘發)된 휜쥐의 간손상(肝損傷)에 마치는 영향(影響)

  • Shin, Mee-Ran (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, SangJi-Univ.) ;
  • Heo, Woon-Yeong (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, SangJi-Univ.) ;
  • Kim, DaI-Rae (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, SangJi-Univ.) ;
  • Jeon, Jong-Weon (Dept. of Multimedia Design, SangJi-YoungSeo College.) ;
  • Kim, Jeong-Yeol (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, SangJi-Univ.)
  • 신미란 (상지대학교 한의과대학 사상체질의학교실) ;
  • 허운영 (상지대학교 한의과대학 사상체질의학교실) ;
  • 김달래 (상지대학교 한의과대학 사상체질의학교실) ;
  • 전종원 (상지영서대학 멀티미디어디자인학과) ;
  • 김정열 (상지대학교 한의과대학 사상체질의학교실)
  • Published : 2005.04.30

Abstract

1. Objectives The present study was carried out to investigate the effects of Chungpyesagan-tang on the $CCI_4-induced$ Liver Damage in Rats. 2. Methods Sprague-Dawley rats were devided into 5 experimental groups : Normal, $NS+CCI_4(Solid$ extract of $CCI_4$ injection group after Normal Saline feed), $CP+CCI_4(Solid$ extract of $CCI_4$ injection group after Chungpyesagantang feed), $CCI_4+NS(Nomal$ Saline feed group after $CCI_4$ injection), $CCI_4+CP(Solid$ extract of Chungpyesagantang feed group after $CCI_4$ injection). Biochemical assays for serum enzyme activities such as AST, ALT, ALP, BUN, Creatinine, Uric Acid, Total Protein, Albumin, Total Cholesterol, Triglyceride, Glucose, and mRNA Revelation of Cytochrome p450 and activities such as LPO(Lipid Peroxidation), GSH(Glutathione), GST(Glutathione-S- Transferase), Glutathione Reductase, Glutathione Peroxidase, SOD(Superoxide Dismutase), Catalase, Hydroxyproline, and ${\beta}-Glucuronidase$ were performed. 3. Results 1) $CP+CCI_4$ showed significantly lower relation of Cytochrome p450 than $NS+CCI_4$. 2) As to LPO Hydroxyproline, $CCI_4+CP$ showed significantly lower activity than $CCI_4+NS$. 3) As to GSH GST Glutathione Peroxidase Catalase, $CP+CCI_4$ showed higher activity than $NS+CCI_4$, $CCI_4+CP$ showed significantly higher activity than $CCI_4+NS$. 4) As to Glutathione Reductase SOD, $CCI_4+CP$ showed significantly higher activity than $CCI_4+NS$. 5) As to ${\beta}-Glucuronidase$, $CP+CCI_4$ showed signficantly lower activity than $NS+CCI_4$. 4. Conclusions Chungpyesagantang has the recovering effects on the $CCI_4-induced$ Liver Damage.

Keywords