대한한방내과학회지 (The Journal of Internal Korean Medicine)
- 제26권1호
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- Pages.129-142
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- 2005
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- 1226-9174(pISSN)
금은화(金銀花)가 천식(喘息)유발 cytokine 분비와 호산구 chemotaxis에 미치는 영향
Lonicerae Flos contributes to the chemotaxis of eosinophils and secretion of cytokines in A549 human epithelial cells
- 정광진 (경희대학교 한의과대학 페계내과학 교실) ;
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정승기
(경희대학교 한의과대학 페계내과학 교실) ;
- 이형구 (경희대학교 한의과대학 페계내과학 교실) ;
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정희재
(경희대학교 한의과대학 페계내과학 교실)
- Chung, Kwang-Jin (Dept. of Internal Medicine Oriental Medicine, Kyunghee University) ;
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Jung, Sung-Ki
(Dept. of Internal Medicine Oriental Medicine, Kyunghee University) ;
- Rhee, Hyung-Koo (Dept. of Internal Medicine Oriental Medicine, Kyunghee University) ;
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Jung, Hee-Jae
(Dept. of Internal Medicine Oriental Medicine, Kyunghee University)
- 발행 : 2005.03.30
초록
Objective : Asthma is known as chronic airway inflammatory disease. This inflammation is conducted by various inflammatory cells including eosihophil. Chemotaxis is one way that circulating inflammatory cells invade a specific lesion. This study examines the degree to which Lonicerae Flos inhibits eosinophil chemotaxis at pulmonary epithelium after allergic stimulation. Material and Methods : Water extracts of Lonicerae Flos and pulmonary epithelial cell lines A549(human type II-like epithelial cells) and human eosinophils were used. Cytotoxic effects of Lonicerae Flos via MTS assay were estimated, as well as the effects of Lonicerae Flos on chemokines from prestimulated A549 cells by sandwich ELISA and RT-PCR. Chemotaxis assay was conducted on prestimulated eosinophils treated with Lonicerae Flos. Result : In this study