Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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Microarray Analysis of Gene Expression in Glioblastoma Cells Treated with Gwakhyangjeonggisan Herbal Acupuncture Solution

곽향정기산 약침액이 유해산소로 손상된 Glioblastoma 세포주의 유전자발현에 미치는 영향

  • Lee, Hong-Seok (Department of Acupuncture, College of Oriental Medicine, Kyung Hee University) ;
  • Yin, Chang-Shik (Department of Acupuncture, CHA Biomedical Center, College of Medicine, Pochon CHA University) ;
  • Koh, Hyeong-Gyun (Department of Acupuncture, College of Oriental Medicine, Kyung Hee University)
  • 이홍석 (경희대학교 한의과대학 침구학교실) ;
  • 인창식 (포천중문의과대학 치바이오메디컬센터 침구과) ;
  • 고형균 (경희대학교 한의과대학 침구학교실)
  • Published : 2005.12.20
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Abstract

Objectives : Neurological disorders have been one of main therapeutic targets of acupuncture. The present study investigated the protective effects of Gwakhyangjeonggisan herbal acupuncture solution (GHAS). Methods : We performed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in glioblastoma cells, and did microarray analysis with cells exposed to reactive oxigen species (ROS) of hydrogen peroxide by 8.0 k Human cDNA, with cut-off level of 2-fold changes in gene expression. Results : MTT assay showed protective effect of GHAS on the glioblastoma cells exposed to hydrogen peroxide. When glioblastoma cells were exposed to hydrogen peroxide, 16 genes were upregulated. When the cells were pretreated with GHAS before exposure to hydrogen peroxide, 22 genes were upregulated. Most of the genes upregulated by hydrogen peroxide stimulation were reversed to downregulation by GHAS. Conclusion : The gene expression changes observed in the present study are supposed to be related to the protective molecular mechanism of GHAS in the glioblastoma cells exposed to ROS stress.

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