Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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Effects of Anemarrhenae Rhizoma on LPS-stimulated Expression of COX-2 and iNOS in mouse BV2 microglial cells

  • Seo, Bong-Won (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Semyung University) ;
  • Kim, Ee-Hwa (Department of Acupoint & Meridian, College of Oriental Medicine, Semyung University) ;
  • Park, Se-Keun (Department of Oriental Food & Nutrition, College of Oriental Medicine, Semyung University) ;
  • Jang, Mi-Hyeon (Department of Physiology, College of Medicine, Kyung-Hee University) ;
  • Choi, Sun-Mi (Department of Medical Research, Korea Institute of Oriental Medicine) ;
  • Lee, Eun-Yong (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Semyung University)
  • Published : 2005.04.20
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Abstract

Anemarrhenae Rhizoma (AR) has been widely used for the treatment of various diseases in Oriental medicine. To investigate whether AR possesses anti-inflammatory effects against lipopolysaccharide (LPS)-induced inflammation in the BV2 microglial cells, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) assay. reverse transcription -polymerase chain reaction (RT-PCR), and prostaglandin E2 (PGE2) assay, and nitric oxide (NO) detection assay were performed. From the present results, AR was shown to suppress PGE2 synthesis and NO production by inhibiting the LPS-stimulated enhancement of cyclooxygenase-2 and inducible nitric oxide synthase expression in BV2 microglial cells. These results suggest that AR may offer a valuable means of therapy in the treatment of inflammatory diseases by attenuating LPS-induced PGE2 synthesis and NO production.

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