Journal of Acupuncture Research
- Volume 22 Issue 2
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- Pages.147-154
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- 2005
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- 2586-288X(pISSN)
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- 2586-2898(eISSN)
Effects of Liriope Platyphylla on LPS-stimulated Expression of COX-2 and iNOS in Mouse BV2 Microglial Cells
- Park, Sang-Heup (Department of Acupuncture & Mexibustion, College of Oriental Medicine, Semyung University) ;
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Kim, Ee-Hwa
(Department of Acupoint & Meridian, College of Oriental Medicine, Semyung University) ;
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Park, Se-Keun
(Department of Oriental Food & Nutrition, College of Oriental Medicine, Semyung University) ;
- Jang, Mi-Hyeon (Department of Physiology, College of Medicine, Kyung-Hee University) ;
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Choi, Sun-Mi
(Department of Medical Research, Korea Institute of Oriental Medicine) ;
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Lee, Eun-Yong
(Department of Acupuncture & Mexibustion, College of Oriental Medicine, Semyung University)
- Published : 2005.04.20
Abstract
Objective: In this study, the effects of Liriope Platyphylla against LPS-induced inflammation was investigated. Methods: Cell viability was determined using the MTT assay. To identify expressions of COX-2 and iNOS mRNA, RT-PCR was performed. Assessment of PGE2 synthesis was performed using the PGE2 immunoassay. Measurement of NO synthesis was performed using the NO detection. Result : The MTT assay revealed that Liriope Platyphylla exerted no significant cytotoxicity in the microglial BV2 cells. RT-PCR analysis revealed that the mRNA levels of COX-2 and iNOS were significantly decreased in the LPS- and 5 mg/ml Liriope Platyphylla treated group. From the PGE2 immunoassay and NO detection, PGE2 and NO synthesis was significantly suppressed in the LPS- and 5 mg/ml Liriope Platyphylla treated group. Conclusion : In these study, Liriope Platyphylla was shown to suppress PGE2 and NO production by inhibiting LPS-stimulated enhancement of COX-2 enzyme activity and iNOS expression. It is very possible that Liriope Platyphylla can offer a valuable mode of therapy for the treatment of brain inflammatory diseases.
Keywords
- Liriope Platyphylla;
- lipopolysaccharide;
- prostaglandin $E_2$;
- nitric oxide;
- inflammation;
- inducible NOS