Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Fluorescent Method for Observing Intravascular Bonghan Duct

형광염색을 이용한 혈관내봉한관의 관찰

  • Lee, Byung-Cheon (Biomedical Physics Laboratory, School of Physics, Seoul National University) ;
  • Baik, Ku-Youn (Biomedical Physics Laboratory, School of Physics, Seoul National University) ;
  • Johng, Hyeon-Min (Biomedical Physics Laboratory, School of Physics, Seoul National University) ;
  • Sung, Baeck-Kyoung (Biomedical Physics Laboratory, School of Physics, Seoul National University) ;
  • Soh, Kyung-Soon (College of Oriental medicine, Semyung University) ;
  • Kang, Dae-In (College of Oriental medicine, Semyung University) ;
  • Soh, Kwang-Sup (Biomedical Physics Laboratory, School of Physics, Seoul National University)
  • 이병천 (서울대학교 자연과학대학 물리학부 한의학물리연구실) ;
  • 백구연 (서울대학교 자연과학대학 물리학부 한의학물리연구실) ;
  • 정현민 (서울대학교 자연과학대학 물리학부 한의학물리연구실) ;
  • 성백경 (서울대학교 자연과학대학 물리학부 한의학물리연구실) ;
  • 소경순 (세명대학교 한의과대학) ;
  • 강대인 (세명대학교 한의과대학) ;
  • 소광섭 (서울대학교 자연과학대학 물리학부 한의학물리연구실)
  • Published : 2005.12.30
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Abstract

Observation of intra-vascular threadlike structures in the blood vessels of rats is reported with the images by differential interference contrast microscope, and fluorescence inverted microscope of the acridine-orange stained samples. The confocal microscope image and the hematoxylin-eosin staining revealed the distinctive pattern of nuclei distribution that clearly discerned the threadlike structure from fibrin, capillary, small venule, arteriole, or lymph vessel. Physiological function of the intra-vascular thread in connection with acupuncture is discussed. Especially, this threadlike duct can be a circulation path for herb-liquid flow, which may provide the scientific mechanism for therapeutic effect of herbal acupuncture.

미분간섭현미경법과 아크리딘-오렌지(acridine-orange) 형광 염색을 이용하여 쥐 혈관 내의 실 모양 구조물을 관찰하였다. 공초점현미경법과 헤마톡실린-에오신(hematoxylin-eosin) 염색을 통해 피브린, 모세혈관, 소정맥, 소동맥 혹은 림프관의 핵 분포와 뚜렷이 구별되는 혈관 내 실 모양 구조물의 핵 분포 패턴을 얻어낼 수 있었다. 이 실 모양 구조물의 생리적 기능을 침술과 연관하여 논의하였다. 특히, 이 실 모양 관을 통한 약물의 흐름이 약침의 기전에 해당할 것이라는 가설을 제기하였다.

Keywords

References

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