동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제19권3호
  • /
  • Pages.785-791
  • /
  • 2005
  • /
  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

Effects of Corydalis Tuber on Synthesis of NO and $PGE_2$ in Murine Macrophage RAW 264.7 Cells Stimulated by LPS

  • Lee, Ki-Young (Department of Acupoint & Meridian, College of Oriental Medicine, Semyung University) ;
  • Park, Se-Keun (Department of Oriental Food & Nutrition, College of Oriental Medicine, Semyung University) ;
  • Kim, Jeong-Seon (Department of Oriental Food & Nutrition, College of Oriental Medicine, Semyung University) ;
  • Jang, Mi-Hyeon (Department of Physiology, College of Medicine, Kyung-Hee University) ;
  • Kim, Chang-Ju (Department of Physiology, College of Medicine, Kyung-Hee University) ;
  • Choi, Sun-Mi (Department of Medical Research, Korea Institute of Oriental Medicine) ;
  • Lee, Hye-Jung (Department of Oriental Medical Science, Graduate School of East & West Medical Science, Kyung-Hee University) ;
  • Kim, Ee-Hwa (Department of Acupoint & Meridian, College of Oriental Medicine, Semyung University)
  • 발행 : 2005.06.25
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초록

Corydalis Tuber has traditionally been used for the treatment of water retention in the body. Administration of the aqueous extract of Corydalis Tuber has been known to be effective for the control of pain and treatment of arthritis. It was reported that Corydalis Tuber possesses anti-inflammatory activity and modulates the intestinal immune system. The effect of Corydalis Tuber against LPS-stimulated expressions of COX-2, iNOS, and $IL-1{\beta}$ in cells of the murine RAW 264.7 macrophages was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR), $PGE_2$ immunoassay, and NO detection. The aqueous extract of Corydalis Tuber was shown to suppress $PGE_2$ production by inhibition on the LPS-stimulated enhancement of COX-2 enzyme activity, $IL-1{\beta}$, and iNOS expression in the RAW 264.7 macrophages. Present results suggest that Corydalis Tuber exerts anti-inflammatory and analgesic effects probably by suppressing of COX-2, iNOS, and $IL-1{\beta}$ expressions, resulting in inhibition of $PGE_2$ synthesis. Corydalis Tuber has anti-inflammatory and analgesic effects probably by suppressing of COX-2, iNOS, and $IL-1{\beta}$ mRNA expressions, resulting in inhibition of $PGE_2$ and NO synthesis.

키워드

참고문헌

  1. Schmidt, H.H., Walter, U. NO at work. Cell. 78(6):919-25, 1994.
  2. Simon, L.S. Role and regulation of cyclooxygenase-2 during inflammation. Am J Med. 31, 106(5B):37S-42S, 1999.
  3. Vane, J.R, Bakhle, Y.S., Botting, R.M. Cyclooxygenases 1 and 2. Ann Rev Pharmacol Toxicol. 38, 97-120, 1998.
  4. Oshima, M., Dinchuk, J.E., Kargman, S.L., Oshima, H., Hancock, B., Kwong, E., Trzaskos, J.M., Evans, J.F., Taketo, M.M. Suppression of intestinal polyposis in Apc delta716 knockout mice by inhibition of cyclooxygenase 2 (COX-2). Cell. 87(5):803-809, 1996.
  5. Subbaramaiah, K., Telang, N., Ramonetti, J.T., Araki, R, Devito, B., Weksler, B.B., Dannenberg, AJ. Transcription of cyclooxygenase-2 is enhanced in transformed mammary epithelial cells. Cancer Res. 56(19):4424-4429, 1996.
  6. Mordan, L.J., Burnett, T.S., Zhang, L.X., Tom, J., Cooney, R.V. Inhibitors of endogenous nitrogen oxide formation block the promotion of neoplastic transformation in C3H 10Tl/2 fibroblasts. Carcinogenesis. 14(8):1555-1559, 1993.
  7. Ohshima, H, Bartsch, H Chronic infections and inflammatory processes as cancer risk factors: possible role of nitric oxide in carcinogenesis. Mutat Res. 305(2):253-264, 1994.
  8. Dinarello, C.A. Biology of interleukin-l. FASEB J. 2(2):108-115, 1988.
  9. Arai, K., Nishida, J., Hayashida, K., Hatake, K., Kitamura, T., Miyajima, A, Arai, N., Yokota, T. Coordinate regulation of immune and inflammatory responses by cytokines. Rinsho Byori. 38(4):347-353, 1990.
  10. Cunningham, E.T. Jr., De Souza, E.B. Interleukin 1 receptors in the brain and endocrine tissues. Immunol Today. 14(4):171-176, 1993.
  11. Ban, E., Marquette, C, Sarrieau, A, Fitzpatrick, F., Fillion, G., Milon, G., Rostene, W., Haour, F. Regulation of interleukin-I receptor expression in mouse brain and pituitary by lipopolysaccharide and glucocorticoids. Neuroendocrinology. 58(5):581-587, 1993.
  12. Dower, SK, Sims, J.E., Cerretti, D.P., Bird, TA The interleukin-I system: receptors, ligands and signals. Chem Immunol. 51, 33-64, 1992.
  13. Takao, T., Tracey, D.E., Mitchell, W.M., De Souza, E.B., Interleukin-I receptors in mouse brain: characterization and neuronal localization. Endocrinology. 127(6):3070-3078, 1990.
  14. Crestani, F., Seguy, F., Dantzer, R. Behavioural effects of peripherally injected interleukin-I: role of prostaglandins. Brain Res. 542(2):330-335, 1991.
  15. Dinarello, CA, Savage, N. Interleukin-I and its receptor. Crit Rev ImmunoI. 9(1):1-20, 1989.
  16. Woolf, C.J., Allchorne, A, Safieh-Garabedian, B., Poole, S. Cytokines, nerve growth factor and inflammatory hyperalgesia: the contribution of tumour necrosis factor alpha. Br J Pharmacol. 121(3):417-424, 1997.
  17. Crofford, L.J., Lipsky, P.E., Brooks, P. Abramson S.B. Simon L.S. Van de Putte L.B. Basic biology and clinical application of specific cyclooxygenase-2 inhibitors. Arthritis Rheum. 43, 413, 2000.
  18. Mitchell, JA, Larkin,S., Williams, T.J. Cyclooxygenase-2: regulation and relevance in inflammation. Biochem Pharmacol. 50, 1535-1542, 1995.
  19. Kobayashi, K., Motohara, T., Honma. A, Takahashi, R., Aihara, M., Sudo, T., Yoshizaki, F. Augmentation of the pharmacological action of Corydalis Tuber by saussurea root in isolated mouse ileum. Yakugaku Zasshi. 121(8):647-651, 2001.
  20. Kubo, M., Matsuda, H., Tokuoka, K., Ma, S., Shiomoto, H Anti-inflammatory activities of methanolic extract and alkaloidal components from Corydalis Tuber. Bioi Pharm Bull. 17(2):262-265, 1994.
  21. Matsuda, H., Tokuoka, K., Wu, J., Tanaka, T., Kubo, M. Inhibitory effects of methanolic extract from Corydalis Tuber against types I-IV allergic models. Bioi Pharm Bull. 18(7):963-967, 1995.
  22. Ahmad, N., Chen, L.C Gordon, MA, Laskin, J.D., Laskin, D.L. Regulation of cyclooxygenase-2 by nitric oxide in activated hepatic macrophages during acute endotoxemia. J Leukoc Biol. 71(6):1005-1011, 2002.
  23. Moncada, S., Higgs, E.A Endogenous nitric oxide: physiology, pathology and clinical relevance. Eur J CIin Invest. 21(4):361-374, 1991.
  24. Seibert, K., Zhang, Y., Leahy, K., Hauser,S., Masferrer, J., Perkins, W., Lee, L., Isakson, P. Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain. Proc Natl Acad Sci91(25):12013-12017, 1994.
  25. Bertolini, A, Ottani, A., Sandrini, M. Dual acting antiinflammatory drugs: a reappraisal. Pharmacol Res. 44(6):437-450, 2001.
  26. Plutzky, J. Inflammatory pathways in atherosclerosis and acute coronary syndromes. Am J Cardiol. 88(8):10-15, 2001.
  27. Hehner, S,P., Heinrich, M., Bork, P,M., Vogt, M., Ratter, F., Lehmann, V., Schulze-Osthoff, K., Droge, W., Schmitz, M,L. Sesquiterpene lactones specifically inhibit activation of NF-kappa B by preventing the degradation of I kappa Balpha and I kappa B-beta. J Biol Chem. 273(3):1288-1297, 1998.
  28. Surh, Y,J., Na, H,K., Lee, J,Y., Keum, Y,S. Molecular mechanisms underlying anti-tumor promoting activities of heat-processed Panax ginseng CA Meyer. J Korean Med Sci. 16, 538-541, 2001.
  29. Wadsworth, T,L., Koop, D,R. Effects of the wine polyphenolics quercetin and resveratrol on pro-inflammatory cytokine expression in RAW 264.7 macrophages. Biochem Pharmacol. 57(8):941-949, 1999.
  30. Laskin, D,L., Pendino, K,J. Macrophages and inflammatory mediators in tissue injury. Annu Rev Pharmacol Toxicol. 35, 655-677, 1995.
  31. De Nardin, E. The role of inflammatory and immunological mediators in periodontitis and cardiovascular disease. Ann Periodontol. 6(1):30-40, 2001.
  32. Shapira, L., Soskolne, W.A., Houri, Y., Barak, V., Halabi, A., Stabholz, A. Protection against endotoxic shock and lipopolysaccharide-induced local inflammation by tetracycline: correlation with inhibition of cytokine secretion. Infect Immun. 64(3):825-828, 1996.