Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Corydalis Tuber on Synthesis of NO and $PGE_2$ in Murine Macrophage RAW 264.7 Cells Stimulated by LPS

  • Lee, Ki-Young (Department of Acupoint & Meridian, College of Oriental Medicine, Semyung University) ;
  • Park, Se-Keun (Department of Oriental Food & Nutrition, College of Oriental Medicine, Semyung University) ;
  • Kim, Jeong-Seon (Department of Oriental Food & Nutrition, College of Oriental Medicine, Semyung University) ;
  • Jang, Mi-Hyeon (Department of Physiology, College of Medicine, Kyung-Hee University) ;
  • Kim, Chang-Ju (Department of Physiology, College of Medicine, Kyung-Hee University) ;
  • Choi, Sun-Mi (Department of Medical Research, Korea Institute of Oriental Medicine) ;
  • Lee, Hye-Jung (Department of Oriental Medical Science, Graduate School of East & West Medical Science, Kyung-Hee University) ;
  • Kim, Ee-Hwa (Department of Acupoint & Meridian, College of Oriental Medicine, Semyung University)
  • Published : 2005.06.25
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Abstract

Corydalis Tuber has traditionally been used for the treatment of water retention in the body. Administration of the aqueous extract of Corydalis Tuber has been known to be effective for the control of pain and treatment of arthritis. It was reported that Corydalis Tuber possesses anti-inflammatory activity and modulates the intestinal immune system. The effect of Corydalis Tuber against LPS-stimulated expressions of COX-2, iNOS, and $IL-1{\beta}$ in cells of the murine RAW 264.7 macrophages was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR), $PGE_2$ immunoassay, and NO detection. The aqueous extract of Corydalis Tuber was shown to suppress $PGE_2$ production by inhibition on the LPS-stimulated enhancement of COX-2 enzyme activity, $IL-1{\beta}$, and iNOS expression in the RAW 264.7 macrophages. Present results suggest that Corydalis Tuber exerts anti-inflammatory and analgesic effects probably by suppressing of COX-2, iNOS, and $IL-1{\beta}$ expressions, resulting in inhibition of $PGE_2$ synthesis. Corydalis Tuber has anti-inflammatory and analgesic effects probably by suppressing of COX-2, iNOS, and $IL-1{\beta}$ mRNA expressions, resulting in inhibition of $PGE_2$ and NO synthesis.

Keywords

References

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