Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Gamgung-tang on Proliferation and cAMP Accumulation of Thyroid Cells

감궁탕이 갑상선세포의 증식과 cAMP 축적에 미치는 영향

  • Kim, Mee-Kyung (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University) ;
  • Shon, Yun-Hee (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University) ;
  • Nam, Kyung-Soo (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University) ;
  • Son, Ok-Lye (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University) ;
  • Kim, Cheorl-Ho (Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University)
  • 김미경 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 손윤희 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 남경수 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 손옥례 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 김철호 (동국대학교 한의과대학 생화학분자생물학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실)
  • Published : 2005.06.25
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Abstract

Abnormal thyroid cell proliferation has a very important role in hypothyroidism. Thyroid stimulating hormone (TSH) stimulates proliferation and maintains differentiated function in thyroid follicular cells. A functioning rat thyroid cell line (FRTL) was used to study the effect of Gamgung-tang (GGT, Glycyrrhizae Radix, black beans, Angelicae Radix and Cnidii Rhizoma) on proliferation and cAMP accumulation of thyrocytes. Proliferation of cell was assessed by DNA synthesis and incorporation of $[^3H]thymidine$ into DNA. The concentration of cAMP was measured simultaneously with growth assessment. Extract of GGT ($0.15{\sim}0.9\;mg/ml$ increased DNA synthesis in a dose-dependent manner. GGT at 0.6 (p<0.05) and 0.9 mg/ml (p<0.01) significantly increased $[^3H]thymidine$ incorporation. A comparable effect was observed with TSH. GGT also enhanced cAMP accumulation. These results indicate that GGT increases the proliferation of thyrocytes and may be considered a promising agent for the treatment of autoimmune thyroid disease.

Keywords

References

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