Journal of Photoscience
- Volume 12 Issue 2
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- Pages.87-93
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- 2005
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- 1225-8555(pISSN)
A New Analytical Method to Determine the Purity of Synthetic Fluorophores using Single Molecule Detection Technique
- Song, Nam-Yoong (Division of Optical Metrology, Korea Research Institute of Standards & Science) ;
- Kim, Hyong-Ha (Organic and Biological Analysis Group, Division of Chemical Metrology and Materials Evaluation, Korea Research Institute of Standards & Science) ;
- Park, Tae-Sook (Division of Optical Metrology, Korea Research Institute of Standards & Science, Molecular/Nano photochemistry & Photonics Laboratory, Department of Chemistry, Chungnam National University) ;
- Yoon, Min-Joong (Molecular/Nano photochemistry & Photonics Laboratory, Department of Chemistry, Chungnam National University)
- Published : 2005.08.31
Abstract
A new assay technique to distinguish between pure compounds and the isomeric mixtures has been suggested using single molecule (SM) fluorescence detection technique. Since the number of emission spots in a fluorophorespread film prepared from a genuine dye solution was determined by experimental condition, the deviation of spot numbers from the expected values could be considered to be an indication of lower purity of the sample solution. The lower limit of sample concentration for this assay was determined to be
Keywords
- Single molecule detection;
- Synthetic biofluorophores;
- Number of emission sports;
- Fluorescence quantum yield