Toxicological Research

  • 제21권3호
  • /
  • Pages.235-240
  • /
  • 2005
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  • 1976-8257(pISSN)
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  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
권호 표지

마우스를 이용한 송화분 섭취의 면역원성 및 안전성 탐색

Immunological Activity and Immunotoxicity of Pine Tree Pollen in Mice

  • 김영옥 (식품의약품안전청 의약품평가부 의약품규격과) ;
  • 김광호 (주식회사 켐온 일반독성실) ;
  • 박현지 (한국생명공학연구원 바이오평가센터 질환동물모델평가연구실) ;
  • 박영철 (대구가톨릭대학교 바이오안전성센터) ;
  • 박성욱 (대구가톨릭대학교 바이오안전성센터) ;
  • 허용 (대구가톨릭대학교 바이오안전성센터)
  • Kim, Young-Ok (Korea Food & Drug Administration, Center for Drug Evaluation, Division of Drug Standard) ;
  • Kim, Kwang-Ho (Chemon Inc., Department of General Toxicology) ;
  • Park, Hyun-Ji (Korea Research Institute of Bioscience & Biotechnology, Experimental Animal Laboratory) ;
  • Park, Yeong-Chul (Catholic University of Daegu, Center for Bio-Safety) ;
  • Park, Sung-Wook (Catholic University of Daegu, Center for Bio-Safety) ;
  • Heo, Yong (Catholic University of Daegu, Center for Bio-Safety)
  • 발행 : 2005.09.01
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초록

Pollen has been used for prevention or treatment of certain diseases such as diabetes arthritis or cancer in traditional medicine. Among various pollens, pine tree pollen is known to relieve hypertension, suppress fatty liver progression, and facilitate the digestion, but its immunological activities are less known. To evaluate immunological reactivities and immunotoxicities of pine tree pollen, BALB/c mice were administered to the poller through oral route. Pine tree pollen suspended in distilled water or extracted with methanol has been administered at the concentration of 0, 10, or 100 mg/kg five days per week for four weeks. Polyclonal activation of splenic T cells with phytohemagglutinins did not induce a significant difference in IL-4 and $IFN_{\gamma}$ production between the pollen-administered mice groups and the control mice. Furthermore, polyclonal activation of splenic B cells with lipopolysaccharides did not result a significant difference in IgG1 and IgG2a production among the groups. These findings imply that the intake of pine tree pollen does not bring any humoral and cellular immune-dysrequlation. Whereas, viability of Listeria monocytogenes was suppressed in the mice administered with 100 mg/kg bw methanol extract, indicating the potential ability of pine tree pollen to enhance cell-mediated immunity mediated by type-1 helper T cells. In addition, aberrant upregulation of plasma IgG1 level was observed in the pollen-administered mice, which suggests a possibility of allergic response induction through the pine tree pollen uptake. Overall, pine tree pollen-mediated modulation of humoral or cellular immunity is worthy of further systematic investigation.

키워드

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