Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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[ $5{\alpha}-Reductase$ ] Inhibitors from Native Plants and their Sebosuppressive Effects in Cultured Human Sebaceous Gland Cells

천연물 유래의 $5{\alpha}-Reductase$ 저해제의 개발과 인체 유래 피지선 세포의 배양을 이용한 피지분비 억제 효과 측정

  • Published : 2005.09.30
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Abstract

This study was performed to develop new cosmeceutical agents with sebosuppressive activity from native plant extracts in Korea. Inhibitory efforts of the extracts on $5{\alpha}-reductase$ (5-AR) were evaluated by enzyme kinetics analysis using UV-spectrophotometric method. Two kinds of enzyme suspensions as 5-AR sources were prepared from rat liver tissue and cultured hSG cells. The sebosuppressive effects were determined by measuring the total lipid quantity produced in cultured hSG cells after incubation with the extracts. As a result, Pinus thunbergii extracts showed the most potent 5-AR inhibitory effects. Its $K_i$ values were 0.0002% and 0.0014% for rat liver 5-AR and human sebaceous gland 5-AR, respectively. Addition of Pinus thunberii extract to hSG cells showed 48% reduction in total lipid production at 0.005% concentration. In conclusion, Pinus thunbergii extracts can be used as a cosmeceutical agent to regulate sebum production and to alleviate the sebum-involved skin diseases, such as acne and seborrheic dermatitis.

본 연구에서는 피지분비 억제 효과를 지닌 신규 화장품 원료를 대한민국 유래의 천연 식물에서 개발하고자 하였다. 다양한 식물 추출물의 5alpha-reductase에 대한 저해효과를 자외선 분광광도계를 이용한 효소 동력학적 분석법을 통하여 평가하였다. 효소 동력학적 분석을 위하여 각각 쥐의 간 조직과 배양된 인체 유래 피지선 세포에서 5alpha-reductase를 추출하였다. 그 결과, 5-AR에 대하여 뛰어난 저해효능을 보이는 3종의 식물 추출물을 선정하였고, 이들의 피지분비 억제 효과를 배양된 인체 유래 피지선 세포를 이용하여 분석하였다. 연구의 결과, 수십 종의 식물 추출물 중 해송 추출물이 가장 강력한 5-AR 저해능을 나타내었으며, 인체 유래 피지선 세포에 대하여 0.005% 농도로 처리한 경우 48%의 피지 생성량 감소 효과를 나타내었다. 해송 추출물은 피지 분비를 감소시켜 여드름이나 지루성 피부염과 같은 피부 질환에 효과를 지닌 화장품의 원료로 사용될 수 있을 것이다.

Keywords

References

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