Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Protein Binding Study of S-Ibuprofen Using High-Performance Frontal Analysis

  • Jin, Longmei (Center for Advanced Bioseperation Technology and Department of Chemical Engineering, Inha University) ;
  • Choi, Du-Young (Center for Advanced Bioseperation Technology and Department of Chemical Engineering, Inha University) ;
  • Liu, Haiyan (Center for Advanced Bioseperation Technology and Department of Chemical Engineering, Inha University) ;
  • Row, Kyung-Ho (Center for Advanced Bioseperation Technology and Department of Chemical Engineering, Inha University)
  • Published : 2005.01.20
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Abstract

High-performance frontal analysis (HPFA) was used for the determination of the binding constant of Sibuprofen to human serum albumin (HSA). This experiment was based on an Inertsil 100 Diol 5 column and sodium phosphate buffer (pH 7.4 and ionic strength of 0.17) as the mobile phase. The mixture of S-ibuprofen and HSA (70 $\mu$M) solution were directly injected into the HPFA column. An injection volume of 200 $\mu$L and a “estricted injection”method were applied to ensure the drug to be eluted as a zonal peak with a plateau. The unbound drug concentration was calculated from the peak height of the zonal peak. Scatchard analysis was used for evaluation of the binding constant (K) and binding affinity (nK) of S-ibuprofen to HSA, and the results were K = 2.833 ${\times}$ 10$^4$ [L mol$^{-1}$], nK = 4.935 ${\times}$ 10$^4$ [L mol$^{-1}$], respectively.

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