International Journal of Industrial Entomology and Biomaterials

  • 제11권1호
  • /
  • Pages.67-70
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  • 2005
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  • 3022-7542(pISSN)
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  • 2586-4785(eISSN)
한국잠사학회 (Korean Society of Sericultural Science)
권호 표지

cDNA Cloning and mRNA Expression of A Cuticle Protein Gene Homo­logue from Protaetia brevitarsis

  • Kim Iksoo (Department of Agricultural Biology, National Institute of Agricultural Science & Technology) ;
  • Choi Yong Soo (College of Natural Resources and Life Science, Dong-A University) ;
  • Lee Eun Mee (Department of Agricultural Biology, National Institute of Agricultural Science & Technology) ;
  • Kim Mi Ae (Department of Agricultural Biology, National Institute of Agricultural Science & Technology) ;
  • Yun Enn Young (Department of Agricultural Biology, National Institute of Agricultural Science & Technology) ;
  • Ahn Mi Young (Department of Agricultural Biology, National Institute of Agricultural Science & Technology) ;
  • Jin Bynng Rae (College of Natural Resources and Life Science, Dong-A University)
  • 발행 : 2005.09.01
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초록

A cuticle protein gene, PbLCP12.1, from the white­spotted flower chafer, Protaetia brevitarsis, was isolated and characterized. The gene contains an ORF of 336 nucleotides capable of encoding a 113 amino acid polypeptide with a predicted molecular mass of 12,138 Da and pI of 4.15. The PbLCP12.1 protein contained a type-specific consensus sequence identifiable in other insect cuticle proteins. The deduced amino acid sequence of the PbLCP12.1 cDNA is most similar to Bombyx mori cuticle protein BmLCP18 (37$\%$ protein sequence identity). Northern blot analysis revealed that PbLCP12.1 showed the epidermis-specific expression.

키워드

참고문헌

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