Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Effects of Nonylphenol on CYP17 and CYP19 Expression in the Ovary of Sprague-Dawley Female Rats

Nonylphenol이 CYP17 및 CYP19발현에 미치는 영향

  • Kim Hee Jin (Laboratory of Molecular Toxicology, College of Pharmacy, Pusan National University) ;
  • Ahn Mee Young (Laboratory of Molecular Toxicology, College of Pharmacy, Pusan National University) ;
  • Kim In Young (Korea Food & Drug Administration, National Institute of Toxicological Research) ;
  • Kang Tae Seok (Korea Food & Drug Administration, National Institute of Toxicological Research) ;
  • Kim Tae Sung (Korea Food & Drug Administration, National Institute of Toxicological Research) ;
  • Kang Il Hyun (Korea Food & Drug Administration, National Institute of Toxicological Research) ;
  • Moon Hyun Ju (Korea Food & Drug Administration, National Institute of Toxicological Research) ;
  • Kil Hoyun (Korea Food & Drug Administration, National Institute of Toxicological Research) ;
  • Kim Soon Sun (Korea Food & Drug Administration, National Institute of Toxicological Research) ;
  • Lee Rhee Da (Korea Food & Drug Administration, National Institute of Toxicological Research) ;
  • Park Kui Lea (Korea Food & Drug Administration, National Institute of Toxicological Research) ;
  • Han Soon Young (Korea Food & Drug Administration, National Institute of Toxicological Research) ;
  • Kim Hyung Sik (Korea Food & Drug Administration, National Institute of Toxicological Research)
  • 김희진 (부산대학교 약학대학) ;
  • 안미영 (부산대학교 약학대학) ;
  • 김인영 (식품의약품안전청 국립독성연구원) ;
  • 강태석 (식품의약품안전청 국립독성연구원) ;
  • 김태성 (식품의약품안전청 국립독성연구원) ;
  • 강일현 (식품의약품안전청 국립독성연구원) ;
  • 문현주 (식품의약품안전청 국립독성연구원) ;
  • 기호연 (식품의약품안전청 국립독성연구원) ;
  • 김순선 (식품의약품안전청 국립독성연구원) ;
  • 이이다 (식품의약품안전청 국립독성연구원) ;
  • 박귀례 (식품의약품안전청 국립독성연구원) ;
  • 한순영 (식품의약품안전청 국립독성연구원) ;
  • 김형식 (식품의약품안전청 국립독성연구원)
  • Published : 2005.09.01
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Abstract

Cytochrome P45O 17$\alpha$-hydroxylase (CYPI 7) and cytorhrome P45O aromata.ie (CYPI 9) are key steroidogenic enzymes in androgen and estrogen synthesis. ThiL study evaluated the effects of nonylphenol (NP) on CYP17 and CYP19 expression in the ovary of Sprague-Dawley rats. All female rats were administered orally with the vehicle (control, corn oil), diethylstilbestrol (DES, 5.0 $\mu$g/kg) and NP (50, 100, or 200 mg/kg/day), which was startinB when they were weaned at 21 days of age for 20 days. Twenty four hours after final dose, the animals were anelthetized with ether. Significant decreases in the uterus (wet weight) were observed with 5.0 $\mu$g/kg/day DES (78$\%$, of control) and 200 mg/kg/day NP (62$\%$ of control), respectively Additionally, ovarian weight was significantly decreased with 5.0 $\mu$g/kg/day DES (63$\%$ of control) and 200 mg/kg/day NP (72$\%$ of control). The serum estradiol levels were sligHtly lower in DES and high dose NP treatment groups, but the 74 levels were not affected by DES and NP. The expression of the ovarian CYP19 gene increased with low doses (50 and 100 mg/kg/day) of NP. while DES and high dose oi NP (200 mg/kg/day) did not affect on the CYP19 mRNA levels. In contrast to the CYP19 gene, the CYP17 gene expreLsion level was significantly down-regulated by the DES and 200 mg/ks/day NP. This result suggestE that NP inhibits ovarian estrogen synthelis by supprelsing CYP17 mRNA efprelsion, And different mechanisml might exist for the expression of Lteroidogenic CYP17 and CYP19 genes in the ovary of Sprague-Dawley rats in response to NP.

Keywords

References

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