Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Exercise on Axonal Regeneration and Growth-associated Protein (GAP­43) Expression Following Sciatic Nerve Injury in Rats

좌골신경 손상 후 운동이 쥐의 축색 재생과 성장관련 단백질(GAP-43) 발현에 미치는 영향

  • Seo Tae-Beom (Research Institute of Sports Medicine, Hannam University, Department of Oriental Medicine Daejon University, Department of Physical Education, Korea University) ;
  • Yoon Sung-Jin (Department of Physical Education, Korea University) ;
  • Kim Kyung-Tae (Department of Physical Education, Korea University) ;
  • Yoon Jae-Suk (Research Institute of Sports Medicine, Hannam University) ;
  • Yoon Jin-Hwan (Research Institute of Sports Medicine, Hannam University) ;
  • Park Sung-Tae (Research Institute of Sports Medicine, Hannam University) ;
  • Han In-Sun (Department of Oriental Medicine, Daejon University) ;
  • Namgung Uk (Department of Oriental Medicine, Daejon University)
  • 서태범 (한남대학교 스포츠의학 연구소, 대전대학교 한의학과, 고려대학교 체육교육학과) ;
  • 윤성진 (고려대학교 체육교육학과) ;
  • 김경태 (고려대학교 체육교육학과) ;
  • 윤재석 (한남대학교 스포츠의학 연구소) ;
  • 윤진환 (한남대학교 스포츠의학 연구소) ;
  • 박성태 (한남대학교 스포츠의학 연구소) ;
  • 한인선 (대전대학교 한의학과) ;
  • 남궁욱 (대전대학교 한의학과)
  • Published : 2005.06.01
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Abstract

Physical activity can improve sensorimotor recovery after peripheral nerve injury. Growth-associated protein 43 (GAP-43) is highly correlated with neuronal development and axonal regeneration and present in large quantities in the axonal growth cone. Using immunofluorescene staining and anterograde and retorgrade techniques, we identified enhanced axonal regrowth in distal stump of the sciatic nerve 3-14 days after crush injury in rats with treadmill training. We also carried out western blot to investigate GAP-43 protein expression in injured sciatic nerve. GAP-43 protein levels were highly induced in the injured sciatic nerve 3, 7 and 14 days compared with sedentary group. Thus, the present data provide a new evidence that treadmill training promoted axonal re-growth after injury and increased GAP-43 protein levels in the regenerating nerve.

Growth-associated protein (GAP-43)은 손상된 신경 재생 시 axonal growth를 확인할 수 있는 가장 널리 알려진 단백질이다. 본 연구의 목적은 좌골신경 손상을 준 쥐에 저강도 운동 수행 후 좌골신경에서 발현되는 GAP-43 단백질의 양을 정량적으로 분석하고, 손상된 부분으로부터 신경섬유의 재생 정도를 조직학적으로 분석함으로써 운동이 말초신경 손상을 가한 쥐에 미치는 영향을 분석하는 것이다. CAP-43의 정량적인 분석은 western blotting을 통해 확인하였고, 좌골신경의 조직학적인 분석은 immunofluorescence staining과 anterograde로 DiI 주입, 척수 내의 motor neuron은 retrograde로 DiI를 주입 한 후 형광현미경에서 관찰하였다. 본 연구에서는 좌골신경이 손상된 쥐에게 운동을 수행시킴으로써 CAP피3단백질이 비운동 그룹보다 더 많은 양이 발현되고, 손상된 부위에서 재생하는 신경섬유의 길이와 양이 더 많은 것을 확인할 수 있었다. 또한 운동을 수행한 그룹에서 DiI에 염색된 motor neuron의 수가 더 많이 증가하는 것을 관찰하였다. 따라서 이러한 결과들은 운동이 좌골신경이 손상된 쥐의 재생을 촉진시킬 것으로 생각된다.

Keywords

References

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