Biomedical Science Letters (대한의생명과학회지)
- Volume 11 Issue 2
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- Pages.109-113
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- 2005
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
Complete Nucleotide Sequence of Cytochrome P450 2El Expressed in the Rat Brain
- Shin Song Woo (Department of Biology, Keimyung University) ;
- You Kwan Hee (Department of Biology, Chungnam National University) ;
- Ryu Hye Myung (Department of Biology, Keimyung University) ;
- Kim Su Won (Department of Biology, Keimyung University) ;
- Kwon Oh Sik (Department of Microbiology, Keimyung University) ;
- Song Jae Chan (Department of Veterinary Medicine, Kyungpook National University) ;
- Kim Myoung Hee (Department of Anatomy, Yonsei University College of Medicine) ;
- Kim Dae Ran (College of Nursing, Dongsan Medical Center, Keimyung University) ;
- Yoo Min (Department of Biology, Keimyung University)
- Published : 2005.06.01
Abstract
From the RT-PCR amplifications using mRNA templates isolated from Sprague-Dawley rat brains, we isolated a cDNA fragment of 1,524 bp which covered the full coding information of the rat brain CYP2El. Its nucleotide sequence was identical to the previously reported rat liver CYP2El mRNA except for the difference of one base (A to C at the nucleotide position 73). This difference also altered the amino acid Lys to GIn. However, no insertion or deletion of nucleotide(s) which could alter the reading frame was found within the structure of this rat brain CYP2El. This study should provide the molecular basis regarding the pathophysiological function of CYP2El in the brain.